In this study a total of 2230 sheep (one-three years of age) were serologically surveyed in three selected areas in Libya (Western, Middle and Southern areas) to specify foci of infection and determination of the prevalence of ovine brucellosis using Rose Bengal Plate Test and Rivanol test. Prevalence of brucellosis in this study revealed 4%, 0%and 0%, respectively. Only the western area showed positive cases, while the Middle and Southern areas showed no serological evidence of brucella infection.

50 specimens of the Mullets Mugil cephalus were collected from the Libian coastal waters near Misurata city in Libya. 7 different species of trematodes isolated from the intestine of the mullets were briefly described. Haploporus benedenii, Haploporus lateralis, Lecithobotryes putrescens, Saccocoelium tensum, Saccocoelium gohari, Haplosplanchnus caudatus and Haplosplanchnus pachysomus. It is worthy to mention that, the present work is reported for the first time in Libya

Anaplasmamarginale (A. marginale) is an obligate intra-erythrocytic rickettsia;it is the cause of anaplasmosis, an important tick-borne disease of cattle. Recovered and vaccinated cattle in endemic areas are apparently normal but remain persistently infected and serve as reservoirs for the parasite.This study intended to detect A. marginale in infected and apparently healthy subclinically infected cattle in North Western Libya. During the period extended from March-2006 till September-2007, blood samples and blood smears were collected from totally 119 adult cow (group – I). These cows were raised at some governmental and private farms in Tripoli, Al-Zawiya and Imssallata districts. Blood smears were stained with May-Grunewald- Giemsa stain and examined under Light microscope to detect the presence of intraerythrocytic bacteria. Indirect- ELISA (IELISA) using a 19 KD A. marginale recombinant antigen was used to detect serologically positive reactors.During the study period, 20 cases of acute anaplasmosis were diagnosed in these farms (Group-II); where, three cows died and two aborted. The Seroprevalence for A. marginale by IELISAwas 64% and 100% in group I and II, respectively. Stained thin blood smears failed to detect infective RBCs in group I, however, variable degrees of parasitaemia were detected in group II. In conclusion, this study approved that serological test (IELISA) was more reliable than direct microscopic examination of stained blood smear in detection of chronic persistent anaplasma-infected cows in endemic areas.

Objective: The present study was primarily undertaken to determine the seroprevalnace of Chlamidya abortus infection in the Libyan camel (Camelus dromedarius). Materials and methods: Serological tests for C. abortus antibodies were conducted with 245 camels (205 females and 40 males) in different localities in the western region of Libya. Animals varied in age from <1 to 20 years and were sampled randomly from both housed and nomadic herds. From each animal, 10 mL blood sample was collected and sera were separated. Antibodies in the sera against C. abortus were detected using an indirect enzyme-linked immunosorbent assay (ELISA). Results: Results of this study showed that out of the 245 camels tested for anti-chlamydia antibodies, 30 were found positive giving an overall prevalence of 12.25%. The seroprevalence of chlamydiosis was 2 folds higher in females (14.00%) as compared to males (5.00%). Conclusion: The present findings signify the C. abortus as a potential agent to cause abortion in Libyan camel (C. dromedarius). Besides, the persons who handle camels in Libya are at risk of infecting with C. abortus. [J Adv Vet Anim Res 2016; 3(2.000): 178-183]