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Determination of macrophage chemotaxis to atherosclerotic plaque extract in domestic turkeys with hypertension
1990
Johnson, P.D. | Klesius, P.H. | Krista, L.M.
The chemotactic activity of turkey peritoneal macrophages in response to an atherosclerotic plaque extract from a hypertensive strain of turkeys was determined. Atherosclerotic plaque extract stimulated macrophage chemotaxis, whereas normal aortic extract did not stimulate macrophage chemotaxis. However, differences were not revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of extracts of atherosclerotic plaque and normal aorta. Chemotactic activity was diminished with pronase treatment, suggesting the chemoattractant is a protein. Seemingly, atherosclerotic plaque of turkeys contains a macrophage chemotaxin.
Show more [+] Less [-]Effects of Mycoplasma hyopneumoniae and Actinobacillus (Haemophilus) pleuropneumoniae infections on alveolar macrophage functions in swine
1990
Alveolar macrophages were collected at necropsy from pigs inoculated with Mycoplasma hyopneumoniae or Actinobacillus pleuropneumoniae or both and were tested for phagocytic capabilities, using in vitro techniques. Macrophages from noninoculated littermates were used as controls. Alveolar macrophages from pigs inoculated with either M hyopneumoniae or A pleuropneumoniae had significantly (P < 0.05 to P < 0.0025) higher phagocytic capacity than that of noninoculated controls. Macrophages from A pleuropneumoniae-inoculated pigs were comparatively more stimulated than were those from M hyopneumoniae-inoculated pigs. Pigs inoculated with M hyopneumoniae and then challenge-exposed with A pleuropneumoniae 2 and 4 weeks later had greatly reduced phagocytosis. Infection with M hyopneumoniae or A pleuropneumoniae caused stimulation of alveolar macrophage functions, and M hyopneumoniae infections may have suppressed phagocytic responses when pigs were challenge-exposed with a secondary pathogen (A pleuropneumoniae). This potential suppression may represent a prediposition of the host by M hyopneumoniae to secondary bacterial infections.
Show more [+] Less [-]Influence of Rhodococcus equi on the respiratory burst of resident alveolar macrophages from adult horses
1990
Brumbaugh, G.W. | Davis, L.E. | Thurmon, J.C. | Savage, D.C.
Opsonized Rhodococcus equi activated the respiratory burst of resident alveolar macrophages (AM) from adult horses in a logarithmic-linear, mass-related manner. The effect of R equi was not significantly different from that of equal masses of opsonized zymosan A. Therefore, R equi does not appear to attenuate the respiratory burst of equine AM. The stimulatory effect of R equi was not reflected by increased production of superoxide anion (O2-), but increased activity of the hexose monophosphate shunt was observed. These results suggest a similarity between the respiratory burst of Am from horses and that of AM from rabbits. We concluded that resident AM from adult horses do not produce O2- concurrently with an increase in activity of the hexose monophosphate shunt when stimulated with either opsonized zymosan A or opsonized R equi. This suggests that O2- is not an important component of the antibacterial defenses of equine AM. Whether equine AM are incapable of producing O2- or require different stimuli to produce it was not determined.
Show more [+] Less [-]Effects of antibiotics on phagocyte recruitment, function, and morphology in the bovine mammary gland during the early nonlactating period
1990
Lintner, T.J. | Eberhart, R.J.
The effects of 2 antibiotic preparations administered intramammarily on phagocyte recruitment, function, an morphology were evaluated at the beginning of the nonlactating period. Twelve cows with no clinical or micro biologic evidence of mastitis were assigned to 1 of 2 treatment groups. At the end of lactation, 1 of the antibiotic preparations was infused in a fore- and hind quarter of each cow; the remaining quarters were untreated controls. One group was given benzathine cephapirin; the second group was given sodium novobiocin. Secretion samples were collected from 1 treated and 1 control quarter at 16 hours, and from the remaining 2 quarters at 64 hours after treatment. Total and differential somatic cell counts were determined, and morphology of mammary polymorphonuclear neutrophils (PMN) and macrophages was observed by transmission electron microscopy. In vitro ingestion and killing of Staphylococcus aureus by mammary PMN and macrophages were assessed by fluorescent microscopy, using acridine orange stain. Cells resident in a fixed volume of secretion were incubated with a known concentration of S aureus. Total cell and PMN concentrations were higher in treated than in control quarters. Neutrophils were the predominant cell type in both treated and control quarters over the sampling period. As measured in this study, in vitro ingestion and killing of S aureus by individual PMN from treated quarters was reduced. Antibiotic treatment also increased the proportion of morphologically abnormal phagocytes. There were significant correlations among PMN ingestion, killing, and morphology. However, increased PMN concentrations tended to compensate for the reduced phagocytic function of individual cells. Therefore, efficacy of antibiotic treatment of nonlactating cows may depend, at least in part, on increased PMN concentration, which may tend to compensate for reduced phagocytic function. Compared with PMN, macrophages appeared to have only a minor role in phagocytosis of bacteria.
Show more [+] Less [-]Disassociation of bactericidal and fungistatic activities from the oxidative burst of avian macrophages
1990
Harmon, B.G. | Glisson, J.R.
Avian peritoneal exudate macrophages, when exposed to phagocytic stimuli, produced an appreciable oxidative burst as measured by production of chemiluminescence, superoxide anion, and hydrogen peroxide. Metabolic inhibitors of the oxidative burst and scavengers of oxygen radicals clearly inhibited macrophage chemiluminescence, but had no significant effect on macrophage bactericidal activity against Escherichia coli or fungistatic activity against Candida tropicalis. Therefore, avian macrophages were capable of oxygen-independent bactericidal and fungistatic activities.
Show more [+] Less [-]Immunohistochemical localization of alpha 2-beta 1-glycoprotein in horses
1990
Winder, N.C. | Pellegrini, A. | Fellenberg, R. von
Alpha 2-beta 1-glycoprotein may be found free in horse serum or complexed with alpha-1-proteinase inhibitor to form pre-alpha 2-elastase inhibitor. There has been little information published concerning alpha 2-beta 1-glycoprotein and its possible tissue sources in horses. A peroxidase-antiperoxidase technique was used to identify alpha 2-beta 1-glycoprotein in buffy coat and bone marrow neutrophils of healthy horses. Macrophages and neutrophils in bronchoalveolar lavage samples from clinically normal horses and from horses with chronic pulmonary disease also were positive for alpha 2-beta 1-glycoprotein. Alpha 2-beta 1-glycoprotein was identified in some instances in normal equine hepatocytes of formalin-fixed liver sections. In formalin-fixed liver sections from horses with chronic, small-airway disease and chronic bronchointerstitial pneumonia, alpha 2-beta 1-glycoprotein was observed in some airway secretions and in macrophages.
Show more [+] Less [-]Effect of bovine herpesvirus-1 or parainfluenza-3 virus on immune receptor-mediated functions of bovine alveolar macrophages in the presence or absence of virus-specific serum or pulmonary lavage fluids collected after virus infection
1990
Brown, T.T. Jr | Shin, K.
The immune receptor-mediated functions of bovine alveolar macrophages (AM) inoculated in vitro with bovine herpesvirus-1 (BHV-1) or parainfluenza-3 (PI-3) virus were tested in the presence or absence of virus-specific antiserum or pulmonary lavage fluids collected from calves 6 days after inoculation with BHV-1 or PI-3 virus. The Fc and C3b phagocytic indices of noninoculated AM, collected from 6- to 16-week-old calves, ranged from 75 to 87 and 59 to 64, respectively, and the binding indices ranged from 5 to 8 and 22 to 28, respectively. Infection of AM with either BHV-1 or PI-3 virus had no significant effect on receptor-mediated phagocytosis or binding, with the exception of a significant (P < 0.05) decrease, from 64 to 46, of the C3b phagocytic index of PI-3 virus-infected AM. The addition of lavage fluids, collected after BHV-1 or PI-3 virus infection, to AM infected with the respective virus caused a significant (P < 0.05) decrease in phagocytic indices with values for the Fc and C3b indices in BHV-1-infected AM decreasing from 81 to 49 and from 47 to 8, respectively, and those for the PI-3 virus-infected AM from 79 to 51 and from 46 to 15, respectively. The binding indices of virus-infected AM increased with the addition of viral lavage fluids, but the only significant (P < 0.05) increase was for C3b binding in PI-3 virus-infected cells, which increased from 33 to 56. Virus-specific serum added to AM infected with the respective virus also caused significant (P < 0.05) decreases in the Fc and C3b phagocytic indices, with those for BHV-1-infected AM decreasing from 81 to 24 and from 47 to 5, respectively, and those for PI-3 virus-infected AM from 79 to 23 and from 46 to 3, respectively. The Fc binding index significantly (P < 0.05) increased with the addition of virus-specific serum from 8 to 34 and from 10 to 42 in BHV-1 and PI-3 virus-infected AM, respectively. The C3b binding index of these AM also increased, but not significantly. Infection of AM with either BHV-1 or PI-3 virus had no significant effect on the phagocytosis of opsonized (OPZ) or nonopsonized (nonOPZ) Staphylococcus epidermidis (SE). The addition of lavage fluids, obtained after BHV-1 infection, to AM infected with BHV-1, significantly (P < 0.05) decreased the percentage of phagocytosis of OPZ-SE from 28 to 21 and had a similar, but less substantial effect, on the phagocytosis of nonOPZ-SE. Lavage fluids collected after PI-3 virus inoculation, added to PI-3 virus-infected AM did not have a notable effect on the phagocytosis of OPZ-SE, but did cause a significant (P < 0.05) decrease in the percentage of phagocytosis of nonOPZ-SE from 25 to 17. The addition of virus-specific serum to infected AM caused significant (P < 0.05) decreases in the percentage of phagocytosis of OPZ-SE and nonOPZ-SE, with the values in the BHV-1-infected AM going from 28 to 11 and 16 to 9, respectively, and in the PI-3-infected AM from 36 to 12 and 25 to 13, respectively. Alveolar macrophages infected with either BHV-1 or PI-3 virus, in the presence or absence of lavage fluids from virus-infected calves or virus-specific serum, killed ingested SE as readily as noninfected AM. On the basis of the findings of this study, we suggest, as with other virus infections, that products of the host antiviral immune response interact with AM infected with BHV-1 or PI-3 virus or cause impaired internalization of receptor-bound particles, resulting in impaired AM antimicrobial functions.
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