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Content of selected amino acids in the gastrocnemius muscle during experimental hypothyroidism in rats Full text
2016
Gołyński, Marcin | Szpetnar, Maria | Tatara, Marcin R. | Lutnicki, Krzysztof | Gołyńska, Magdalena | Kurek, Łukasz | Szczepanik, Marcin | Wilkołek, Piotr
Content of selected amino acids in the gastrocnemius muscle during experimental hypothyroidism in rats Full text
2016
Gołyński, Marcin | Szpetnar, Maria | Tatara, Marcin R. | Lutnicki, Krzysztof | Gołyńska, Magdalena | Kurek, Łukasz | Szczepanik, Marcin | Wilkołek, Piotr
Introduction: Thyroid hormones affect protein turnover, and in the case of hypothyroidism a decrease in protein synthesis and reduced release of certain amino acids from skeletal muscles are observed. Changes in the amino acid system of skeletal muscles may be responsible for the occurrence of muscle disorders. Material and Methods: The study measured the content of selected amino acids in the gastrocnemius muscle of Wistar rats during experimental hypothyroidism induced by oral administration of methimazole at a concentration of 0.05% in drinking water for 90 d. The rats were divided into four groups: E1 (n = 6) - experimental males, E2 (n = 6) - experimental females, C1 (n = 6) - control males, and C2 (n = 6) control females. Results: A statistically significant reduction occurred in leucine, isoleucine, and 1-methylhistidine levels in males, and 1-methylhistidine in females, in comparison to the control groups. Conclusion: The hypothyroidism-induced changes in amino acid content may be responsible for the occurrence of skeletal muscle function disorders.
Show more [+] Less [-]Content of selected amino acids in the gastrocnemius muscle during experimental hypothyroidism in rats Full text
2016
Gołyński Marcin | Szpetnar Maria | Tatara Marcin R. | Lutnicki Krzysztof | Gołyńska Magdalena | Kurek Łukasz | Szczepanik Marcin | Wilkołek Piotr
Introduction: Thyroid hormones affect protein turnover, and in the case of hypothyroidism a decrease in protein synthesis and reduced release of certain amino acids from skeletal muscles are observed. Changes in the amino acid system of skeletal muscles may be responsible for the occurrence of muscle disorders. Material and Methods: The study measured the content of selected amino acids in the gastrocnemius muscle of Wistar rats during experimental hypothyroidism induced by oral administration of methimazole at a concentration of 0.05% in drinking water for 90 d. The rats were divided into four groups: E1 (n = 6) - experimental males, E2 (n = 6) - experimental females, C1 (n = 6) - control males, and C2 (n = 6) control females. Results: A statistically significant reduction occurred in leucine, isoleucine, and 1-methylhistidine levels in males, and 1-methylhistidine in females, in comparison to the control groups. Conclusion: The hypothyroidism-induced changes in amino acid content may be responsible for the occurrence of skeletal muscle function disorders.
Show more [+] Less [-]The preventive role of Spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model Full text
2021
Seyidoğlu, Nilay | Köşeli, Eda | Gurbanlı, Rovshan | Aydın, Cenk
The preventive role of Spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model Full text
2021
Seyidoğlu, Nilay | Köşeli, Eda | Gurbanlı, Rovshan | Aydın, Cenk
There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism’s internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga Spirulina platensis (Arthrospira platensis) in a stress-induced rat model, and to describe its possible mechanism of action. Thirty-six adult male Sprague Dawley rats were separated into four groups: control (C), stress (S), S. platensis (Sp), and S. platensis + stress (SpS). The rats in groups Sp and SpS were fed with 1,500 mg/kg b.w./day Spirulina platensis for 28 days. All rats were exposed to prolonged light phase conditions (18 h light : 6 h dark) for 14 days. The SpS and S groups were exposed to stress by being kept isolated and in a crowded environment. Blood samples were obtained by puncturing the heart on the 28th day. The effect of stress on serum corticosterone, oxidative stress markers (TOS, TAC, PON1, OSI) and immunological parameters (IL-2, IL-4, IFN-ɣ) were tested. Also, the brain, heart, intestines (duodenum, ileum, and colon), kidney, liver, spleen, and stomach of the rats were weighed. Serum corticosterone levels were higher in the S group than in the C group, and significantly lower in the SpS group than in the S group. Mean total antioxidant capacity were lower in the S group than in the C group, and Spirulina reversed this change. Although not significantly different, IL-2 was lower in the S group than in the C group. However, in the SpS group, IL-2 increased due to Spirulina platensis mitigating effects of stress. Male rats fed a diet with Spirulina platensis could experience significantly milder physiological changes during stress, although stress patterns may be different. Exogenous antioxidant supplements merit further investigation in animals and humans where the endogenous defence mechanism against stress may not be sufficient.
Show more [+] Less [-]The preventive role of Spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model Full text
2021
Seyidoğlu Nilay | Köşeli Eda | Gurbanlı Rovshan | Aydın Cenk
There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism’s internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga Spirulina platensis (Arthrospira platensis) in a stress-induced rat model, and to describe its possible mechanism of action.
Show more [+] Less [-]Redescription of Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin, 1991 (Nematoda: Amidostomatidae), a parasite of Fulica atra (Gruiformes) Full text
2020
Królaczyk, Katarzyna | Zaborski, Daniel | Dzierzba, Emil | Kavetska, Katarzyna M.
Redescription of Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin, 1991 (Nematoda: Amidostomatidae), a parasite of Fulica atra (Gruiformes) Full text
2020
Królaczyk, Katarzyna | Zaborski, Daniel | Dzierzba, Emil | Kavetska, Katarzyna M.
Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin, 1991, is a species of which the systematic position is still unclear, and it is reported in the literature under many synonyms. In the present study, an attempt has been made at establishing the ultimate systematic position of Quasiamidostomum fulicae against the backdrop of selected Amidostomatinae species. The parasites were identified based on measurements of external and internal structures. Ecological analysis of Q. fulicae was carried out using the quantitative indices (frequency, prevalence, mean intensity, relative abundance, and dominance index). Statistical analyses (discriminant analysis) were performed on measurement data. The intestines of 77 coots were examined. They yielded a total of 398 parasites, including 67 identified as Q. fulicae. Both males and females were located in the muscular gizzard. The morphometric analysis of Q. fulicae in this study showed the dimensions of all the internal organs to be in agreement with measurements reported by other authors. The discriminant analysis, used to find the differences between the examined nematode species (Amidostomoides acutum, A. petrovi, A. monodon, Amidostomum anseris, and Quasiamidostomum fulicae), gave highly significant results (P < 0.0001) with respect to both males and females. The results justify the separation of Q. fulicae from the genus Amidostomum.
Show more [+] Less [-]Redescription of Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin, 1991 (Nematoda: Amidostomatidae), a parasite of Fulica atra (Gruiformes) Full text
2020
Królaczyk Katarzyna | Zaborski Daniel | Dzierzba Emil | Kavetska Katarzyna M.
Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin, 1991, is a species of which the systematic position is still unclear, and it is reported in the literature under many synonyms. In the present study, an attempt has been made at establishing the ultimate systematic position of Quasiamidostomum fulicae against the backdrop of selected Amidostomatinae species.
Show more [+] Less [-]First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Full text
2019
Filip-Hutsch, Katarzyna | Hutsch, Tomasz | Kolasa, Szymon | Demiaszkiewicz, Aleksander W.
First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Full text
2019
Filip-Hutsch, Katarzyna | Hutsch, Tomasz | Kolasa, Szymon | Demiaszkiewicz, Aleksander W.
Parafasciolopsis fasciolaemorpha is a liver fluke typically parasitising moose in Central and Eastern Europe. The aim of our studies was to describe a case of fatal moose parafasciolopsosis, with special emphasis on the histopathological changes caused in the liver tissue by around 10,000 flukes. A male moose, found dead in Polesie National Park, eastern Poland, was subjected to parasitological necropsy. Macroscopic and histopathological examination of the liver was performed. Over 10,000 flukes identified as P. fasciolaemorpha were isolated from the liver parenchyma. Histopathological examination of the liver revealed the presence of multiple cavities, which were filled with flukes and cellular detritus and encysted with a layered capsule of connective tissue. Extensive liver fibrosis with signs of incomplete septal cirrhosis was also observed. Parafasciolopsosis with accompanying diarrhoea was the most probable reason for the moose’s death. However, it is possible that most moose are able to survive extremely intensive P. fasciolaemorpha infection by formation of extensive fibrosis, which isolates flukes from the liver parenchyma and therefore retards the failure of the organ. To the best of our knowledge, this is the first histopathological description of changes in the liver of a moose infected with P. fasciolaemorpha.
Show more [+] Less [-]First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Full text
2019
Filip-Hutsch Katarzyna | Hutsch Tomasz | Kolasa Szymon | Demiaszkiewicz Aleksander W.
Parafasciolopsis fasciolaemorpha is a liver fluke typically parasitising moose in Central and Eastern Europe. The aim of our studies was to describe a case of fatal moose parafasciolopsosis, with special emphasis on the histopathological changes caused in the liver tissue by around 10,000 flukes.
Show more [+] Less [-]Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Full text
2018
Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Full text
2018
Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks. A total of 136 Ixodes ricinus ticks, collected in the Ternopil (Ukraine) region, including 126 adults (70 females and 56 males), and 10 nymphs were examined. The identification of the species and their developmental form was based on morphological characteristics. PCR with B5S-Bor and 23S-Bor primers resulted in Borrelia burgdorferi sensu lato DNA amplification among six ticks (4.4%). The detailed analysis based on the DNA sequencing showed the presence of DNA of Borrelia afzelii in four samples; the remaining two represented Borrelia burgdorferi sensu lato complex, although their genospecies were not determined. The research confirmed the dominance of Borrelia afzelii genospecies in the ticks from Ukraine. It seems reasonable to undertake similar research in ticks from other regions of Ukraine. Knowledge in this field can be useful for public health and planning the prevention of tick-borne diseases.
Show more [+] Less [-]Prevalence of Borrelia burgdorferi sensu lato in ticks from the Ternopil region in Ukraine Full text
2018
Weiner Marcin | Żukiewicz-Sobczak Wioletta | Tokarska-Rodak Małgorzata | Plewik Dorota | Pańczuk Anna | Siłuch Marta | Zagórski Jerzy | Sobczak Paweł | Chmielewski Tomasz | Tylewska-Wierzbanowska Stanisława | Shkilna Mariia | Korda Mykhailo | Klisch Ivan | Andreychyn Mykhailo | Pavliuk Mariana
Lyme borreliosis/Lyme disease is caused by Borrelia burgdorferi and is one of the most common vector-borne diseases transmitted by ticks.
Show more [+] Less [-]Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kałuża, Ewelina | Cepiel, Alicja | Pasławska, Urszula
Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kałuża, Ewelina | Cepiel, Alicja | Pasławska, Urszula
Introduction: The prevalence of arrhythmias in dogs and the influence of sex, breed, age, and body weight were analysed over a seven-year span. Material and Methods: In total, 1189 referrals for cardiological examination by electrocardiography were received at one academic centre in Poland between 2008 and 2014. The largest proportion of the examined dogs were cross-breeds with body weight below 25 kg (n = 153, 12.87%), followed by German Shepherds (n = 122, 10.26%), Labrador Retrievers (n = 68, 5.72%), Yorkshire Terriers (n = 63, 5.3%), and Boxers (n = 60, 5.05%). Retrospective analysis was made of 1201 standing or right recumbent electrocardiograms without pharmacological sedation. The prevalence of arrhythmias was examined in terms of sex, age, body weight, and breed of the dogs. Results: A total of 630 (52.46%) electrocardiograms showed no signs of arrhythmia, but 96 (7.99%) and 475 (39.55%) pointed to physiological and pathological arrhythmias respectively. The most commonly diagnosed type was atrial fibrillation with 33.68% incidence, followed by ventricular arrhythmias (28%), sinus pauses (27.58%), supraventricular arrhythmias (24%), and atrioventricular blocks (22.95%). Pathological arrhythmias were most commonly found in male dogs and in German Shepherds. Conclusions: Atrial fibrillation predominated, followed by premature ventricular complexes. Male dogs were generally more prone to heart rhythm disturbances.
Show more [+] Less [-]Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak Agnieszka | Michałek Marcin | Kałuża Ewelina | Cepiel Alicja | Pasławska Urszula
Introduction: The prevalence of arrhythmias in dogs and the influence of sex, breed, age, and body weight were analysed over a seven-year span.
Show more [+] Less [-]Mitral regurgitation in Dachshund dogs without heart murmurs Full text
2017
Garncarz, Magdalena | Parzeniecka-Jaworska, Marta | Hulanicka, Magdalena | Jank, Michał | Szaluś-Jordanow, Olga | Kurek, Anna
Mitral regurgitation in Dachshund dogs without heart murmurs Full text
2017
Garncarz, Magdalena | Parzeniecka-Jaworska, Marta | Hulanicka, Magdalena | Jank, Michał | Szaluś-Jordanow, Olga | Kurek, Anna
Introduction: Older small breed dogs are considered at risk for heart failure secondary to chronic mitral valve disease. However, few data are available on the onset of this disease in such dogs. This study was performed to determine if auscultation alone can be used to eliminate clinically relevant mitral valve regurgitation seen in echocardiography in Dachshund dogs. Material and Methods: Clinical and echocardiographic data were obtained from 107 dogs without heart murmurs. Results: The study revealed that 63.6% of the dogs had mitral regurgitation. Numbers increased with age and a larger percentage of male Dachshunds were affected than female Dachshunds. Mitral valve prolapse and thickening were mild, and the regurgitant area inextensive in most dogs. Conclusions: The study shows that mitral valve regurgitation is prevalent (63.6%) in Dachshunds without heart murmurs. Typical lesions often become apparent during echocardiographic examinations in dogs under 5 years of age.
Show more [+] Less [-]Mitral regurgitation in Dachshund dogs without heart murmurs Full text
2017
Garncarz Magdalena | Parzeniecka-Jaworska Marta | Hulanicka Magdalena | Jank Michał | Szaluś-Jordanow Olga | Kurek Anna
Introduction: Older small breed dogs are considered at risk for heart failure secondary to chronic mitral valve disease. However, few data are available on the onset of this disease in such dogs. This study was performed to determine if auscultation alone can be used to eliminate clinically relevant mitral valve regurgitation seen in echocardiography in Dachshund dogs. Material and Methods: Clinical and echocardiographic data were obtained from 107 dogs without heart murmurs. Results: The study revealed that 63.6% of the dogs had mitral regurgitation. Numbers increased with age and a larger percentage of male Dachshunds were affected than female Dachshunds. Mitral valve prolapse and thickening were mild, and the regurgitant area inextensive in most dogs. Conclusions: The study shows that mitral valve regurgitation is prevalent (63.6%) in Dachshunds without heart murmurs. Typical lesions often become apparent during echocardiographic examinations in dogs under 5 years of age.
Show more [+] Less [-]Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats Full text
2016
Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats Full text
2016
Introduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.
Show more [+] Less [-]Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats Full text
2016
Mansouri Esrafil | Asadi-Samani Majid | Kooti Wesam | Ghasemiboroon Maryam | Ashtary-Larky Damoon | Alamiri Fatima | Afrisham Reza | Noohi Zahra Hasanzadeh
Introduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.
Show more [+] Less [-]Assessment of luteinizing hormone receptor expression in structural support tissues of canine hip and femorotibial joints Full text
2020
Kiefel, Claire A. | Kutzler, Michelle A.
OBJECTIVE To determine whether luteinizing hormone receptors (LHRs) are expressed in canine femoral head subchondral bone (FHSB), hip joint round ligament (RL), cranial cruciate ligament (CCL), and femorotibial joint synovium (FJS) specimens. SAMPLE 1 specimen each of the FHSB, RL, CCL, and FJS obtained from the left hind limbs of 19 fresh canine cadavers. PROCEDURES 1 section of each FHSB, RL, CCL, and FJS specimen was processed with rabbit polyclonal IgG anti-human LHR antibody, and 1 section was treated with negative control reagents. Percentage immunoexpression of LHRs in FHSB and FJS sections was analyzed by assessment of 100 bone marrow cells or synoviocytes in 3 adjacent hpf (400×). In each RL and CCL section, immunoexpression of LHRs in fibrocytes was semiquantitatively analyzed on the basis of the mean of the product of percentage staining score (from 0 [no staining] to 3 [> 50% of cells stained]) and staining intensity score (from 0 [no staining] to 2 [moderate to strong staining]) for 3 adjacent hpf. RESULTS All tissues examined had variable LHR expression. Expression of LHRs in FHSB, CCL, or FJS specimens did not differ between sexes or between sexually intact and gonadectomized dogs. However, RL specimens from female dogs had significantly greater LHR expression scores, compared with findings for male dogs. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that LHRs are expressed in structural support tissues of canine hip and femorotibial joints. Further research is required to determine the LHRs' function, mechanism of action, and potential contribution to the pathogenesis of hip dysplasia or CCL rupture in dogs.
Show more [+] Less [-]Evaluation of a multipump air pistol as a method for euthanizing young dairy goat kids Full text
2020
Hempstead, Melissa N. | Fowler, Jennifer L. | Shearer, Jane K. | Plummer, Paul J.
OBJECTIVE To assess the extent of damage to the skull and brain of cadaveric dairy goat kids caused by a .22-caliber, 16-g pellet fired from a multipump air pistol at various power levels. SAMPLE Cadavers of 8 male and 7 female dairy goat kids ≤ 5 days old. PROCEDURES Each cadaver was positioned in sternal recumbency with the head and neck extended on a straw bale. A multipump air pistol was held with the barrel perpendicular to and 2.5 cm from the head at the intersection of 2 imaginary lines that extended from the lateral canthus of each eye to the middle of the contralateral ear base and fired at half (5 pumps; n = 2), intermediate (7 pumps; 2), or full (10 pumps; 11) power. The head and neck were removed from the carcass for CT imaging and gross sectioning to determine the location of the pellet and extent of damage caused to the skull and brain. RESULTS The pellet successfully penetrated the skull of all 13 heads shot at full or intermediate power and 1 of the 2 heads shot at half power. The pellet did not fragment after entering the skull of any cadaver and penetrated the brainstem (necessary for instantaneous death) in only 7 cadavers. CONCLUSIONS AND CLINICAL RELEVANCE The described technique was insufficient for use as a stand-alone method for euthanizing young dairy goat kids. Modification of the technique warrants further research to determine whether air pistols can be used to effectively euthanize young goat kids.
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