Refine search
Results 1-2 of 2
Immunohistochemistry for parathyroid hormone-related protein (PTHrP) in benign and malignant mammary mixed tumors of dogs with and without hypercalcemia
2000
Konno, A. (Hokkaido Univ., Sapporo (Japan)) | Sukegawa, A. | Kusano, M. | Kariya, K. | Ishida, T. | Okada, H.
We evaluated the expression of parathyroid hormone-related protein (PTHrP) by immunohistochemistry in eight benign and malignant mammary mixed tumors of dogs with (n = 4) and without (n = 4) hypercalcemia. Positive immunoreactive staining for PTHrP was observed in all four tumors from hypercalcemic dogs. The mammary tumors from 2 of the 4 normocalcemic dogs stained positively for PTHrP, but the numbers of immunoreactive cells and intensity of the immunoreaction were less than in the hypercalcemic dogs. In the other 2 tumors without hypercalcemia, the tissue samples were negative for PTHrP
Show more [+] Less [-]Leptin inhibits hepatocyte growth factor-induced ductal morphogenesis of bovine mammary epithelial cells
2007
Yamaji, D.(Hokkaido Univ., Sapporo (Japan)) | Kamikawa, A. | Soliman, M.M. | Ito, T. | Ahmed, M.M. | Makondo, K. | Watanabe, A. | Saito, M. | Kimura, K.
We examined the effect of stroma-derived factors, hepatocyte growth factor (HGF) and leptin, on morphological differentiation of bovine mammary epithelial cells (BMEC) in collagen gel three-dimensional culture in vitro. BMEC treated with HGF, but not leptin, formed duct-like organoids. The formation of organoids by HGF was enhanced by treatment with a mixture of insulin, cortisol and prolactin, while BMEC treated with the mixture alone did not produce the organoid. In contrast, the formation of organoids by HGF was dose-dependently inhibited by simultaneous addition of leptin, regardless of the presence or absence of the hormone mixture. These results suggest that stroma-derived factors intricately regulate mammary epithelial morphogenesis.
Show more [+] Less [-]