An in vitro bioassay system was used to study the effects of cyclopiazonic acid (CPA) mycotoxin on cardiac muscle. Acute exposure to 6 microgram of CPA/ml of modified Krebs-Henseleit solution significantly (P < 0.05) decreased 5 in vitro turkey cardiac muscle performance criteria: maximal weight a muscle could lift; maximal contraction velocity; relaxation velocity; time to peak contraction; and total time for muscle contraction and relaxation. The effect on these 5 criteria appeared to result from intracellular changes partially associated with calcium availability and were irreversible, suggesting that physiologic changes had developed after acute exposure to CPA.

The relationship of serum complement activity and bacteriostatic activity was investigated in male guinea pigs given aflatoxin and/or rubratoxin. In experiment 1, guinea pigs were given 0.6 mg of aflatoxin/kg of body weight, PO, once. In experiment 2, guinea pigs were given 0.02 mg of aflatoxin/kg, PO, and/or 8 mg of rubratoxin, PO, 11 times. Aflatoxin (0.02 mg/kg) had no effect given alone, but potentiated the effect of rubratoxin. In both experiments, changes in complement activity were accompanied by similar but not always significant (P less than 0.05) changes in bacteriostatic activity of serum. Guinea pigs given 0.06 mg of aflatoxin/kg had significant (P less than 0.05) changes in complement titers and in serum alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities. Guinea pigs given repeated oral doses of aflatoxin and/or rubratoxin had changes in complement titers, bacteriostasis, and alkaline phosphatase and aspartate aminotransferase activities, but not in alanine aminotransferase activities. Significant differences were detected only when average values for all guinea pigs given rubratoxin or rubratoxin with aflatoxin were compared with average values for guinea pigs not given rubratoxin.

In the conditions of the Republic of Belarus there was analyzed the influence of mycotoxins on state of energy metabolism and development of energy deficient states of bred and milking sows maintained in the production breeding conditions. Clinical status of sows was characterized by concurrent mycotoxicoses. As a result of action of mycotoxins in sows there was developed an energy deficiency and state of acidosis. The mentioned above changes played an important part in the pathogenesis of mycotoxicosis. There were presented the following results: analysis of groups of pregnant sows and suckling-pigs; the content of mycotoxins in mixed fodders; symptoms revealed at sows and pigs; indexes of carbohydrate and lipide exchanges in blood of sows; the content of trace elements in blood of sows; gas composition of sow blood. | Исследования проведены на супоросных и подсосных свиноматках, содержащихся в условиях промышленной технологии. Клинический статус свиноматок характеризовался сочетанным микотоксикозом. В результате действия микотоксинов у свиноматок развились энергодефицит и состояние ацидоза. Данные изменения являются важной составной частью патогенеза при микотоксикозах. В табличной форме представлены группы супоросных и подсосных свиноматок, содержание микотоксинов в комбикормах, симптомы, выявленные у свиноматок и поросят, показатели углеводного и липидного обменов в крови свиноматок, содержание макроэлементов в крови свиноматок, газовый состав крови свиноматок.

In the conditions of the Republic of Belarus there was analyzed the efficiency of application of an adsorbent mycotoxins Toxfin Dry (Belgium) for lowering of toxicity load on broiler chickens. In the course of the study there was given attention to poultry productivity, viability and forage expenses for broiler chickens breeding. There was developed a scheme of Toxfin Dry preparation feeding to broiler chickens. There were presented the results of weighing of control and experimental broiler chickens during the breeding periods; the basic zootechnical indices in the conditions of introduction Toxfin Dry; economic efficiency of its application. Research results showed that introduction of a mycotoxin adsorbent Toxfin Dry into diets of broiler chickens promoted the improvement of digestion and absorption of mixed fodder nutrients in a gastroenteric tract, that in its turn promoted the increasing daily average weight gain on 4,3%, broiler stock viability - on 2,6%; and to decrease the expenses for forages on 13,9%. On the basis of obtained experimental data it was recommended to include the mycotoxin adsorbent Toxfin Dry into rations of broiler chickens as a dry mix with mixed fodder in a dose of 5 g/kg of forage dry matter.