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Isolation and morphological characterization of natural killer cell in the Sprague-dawley (SD) rats.
1992
Kang K.S. | Lee Y.S.
Isolation of infectious pancreatic necrosis virus from rainbow trout in Korea.
1991
Lyoo Y.S. | Chang C.H. | Jeon Y.H. | Lee J.O. | Rhee J.C.
Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland
2018
Pyz-Łukasik, Renata | Paszkiewicz, Waldemar
The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers. The study included 24 fish representing grass carp, bighead carp, Siberian sturgeon, and wels catfish. Specimens were collected in winter. Aerobic bacteria, psychrophilic, Enterobacteriaceae, Staphylococcus spp., and E. coli counts were made, and the presence of Salmonella spp., L. monocytogenes, S. aureus, and other coagulase-positive staphylococci was investigated. The microbiological analysis showed a similar level of aerobic, psychrophilic, and Staphylococcus spp. contamination of the four fish species. The Enterobacteriaceae count was higher in the muscles of grass carp and bighead carp than S. sturgeon and wels catfish. No pathogenic bacteria such as Salmonella spp., E. coli, L. monocytogenes, Staphylococcus aureus, or other coagulase positive staphylococci were found in samples of the examined fish species. The fresh fish examined in this study were of good microbiological quality and there was no health risk for consumers.
Show more [+] Less [-]Occurrence of tetracyclines, sulphonamides and quinolones residues in chicken meat sample from selected chicken slaughterhouses in Peninsular Malaysia
2018
Chai, L. C. | Nor Ainy M. | Marni S. | Marzura M. R. | Ungku Fatimah U. Z. A. | Khairunnisak M.
The occurrence of veterinary drug residues in chicken meat originating from 320 small and medium scale chicken slaughterhouses in Peninsular Malaysia was determined. 637 chicken meat samples were examined for tetracycline (TCs), sulphonamide (SAs) and quinolone residues using a microbiological inhibition test and was further confirmed using liquid chromatography mass spectrometry (LCMS/MS). The presence of TC residues were confirmed in 10 (1.6%) samples, and 1 (0.2%)sample was confirmed in compliance to the established maximum residue limit (MRL) for residues of quinolone. A total of 6 (0.9%) samples were above the MRL for TC. The samples were from Pulau Pinang, Terengganu and Kelantan. Among those tested in compliance, the main analytes found for TC and quinolone werechlortetracyclines (CTC), enrofloxacin and mixture of chlortetracycline (CTC) and oxytetracycline (OTC). No samples were found to contain sulfonamides residues.
Show more [+] Less [-]Microbiological Evaluation of Some Farmed Fish Species Marketed in Sharkia Governorate, Egypt
2023
Hania E. Abd El Maksod | Saad M. Saad | Maha M. Samir
The present study was conducted to evaluate the microbiological status of some farmed fish species marketed in Sharkia Governorate then compared with Egyptian standard of chilled fish. keeping quality parameters also examined (pH, TVN, TMA and TBA) and compared with Egyptian standard of chilled fish. A total of 150 samples of farmed fish of Nile cages, concrete ponds and Earthen ponds 50 of each (25 Tilapia nilotica and 25 Mugil cephalus) respectively from the market in Sharkia governorate were examined during autumn 2021. For keeping quality parameters, all examined samples were within the acceptable limits according to ES (3494:2005). The obtained results of microbiological analysis revealed that the percentage of the exceeded permissible limits of aerobic plate count (APC) was 12% and 32%,20% and 16% ,12% and 20% for Tilapia nilotica and Mugil cephalus in Nile cages, concrete ponds and Earthen ponds respectively. For total coliform count, all positive examined samples exceed permissible limits of coliform count (2 log10 CFU/g) according to ES (3494:2005). The incidence of Staphylococcus aureus was 0% and 11%, 36%, and 32%, 16% and 12% for Tilapia nilotica and Mugil cephalus in Nile cages, concrete ponds and Earthen ponds respectively. Listeria species were detected in 20% and 8% ,8% and 16% , 20% and 0% for Tilapia nilotica and Mugil cephalus in Nile cages ,concrete ponds and Earthen ponds, respectively. Escherichia coli was 24% and 20% ,8% and 16%, 16% and 20% for Tilapia nilotica and Mugil cephalus in Nile cages, concrete ponds and Earthen ponds respectively. Salmonella species were 4% and 12% for both from Nile cages and from concrete ponds and 4% and 12% in earthen pond Tilapia nilotica and Mugil cephalus respectively. Serological identification of E. coli, Salmonella and Listeria in these samples was showed in this search. So hygienic and proper practices performed during transportation and handling of fish are needed before consumption of this fish.
Show more [+] Less [-]The Microbiological Quality of Tantuni
2019
Selçuk, Ümit | Ağaoğlu, Sema
In this study, microbiological quality of tantuni that have been consumed in the province of Van was examined. Materials and Methods: For this purpose; 100 tantuni samples, whose 79 of them were red meat tantuni (raw and cooked) and 21 of them chicken tantuni (raw and cooked) were used as material.According to analysis findings with regard to aerobic mesophilic organisms, coliform group microorganisms, E. coli, micrococcus/staphylococcus, S. aureus, C. perfringens and yeast-mould at the samples of raw and cooked red meat tantuni were found to be 5.67 and 3.98, 2.88 and 0.21, 0.87 and 2.00, 2.99 and 2.27, 1.33 and 0.25, 0.05 and 1.00, 4.43 and 0.63 log cfu/g respectively. In the same order, at the samples of raw and cooked chicken tantuni were found to be; 4.35 and 3.77, 2.84 and 1.00, 1.15 and 2.00, 0.95 and 1.22, 2.00 and 2.00, 1.00 and 1.00, 4.05 and 0.11 log cfu/g respectively. Salmonella spp. could not be isolated in the tantuni samples that had been investigated. In the raw red meat tantuni samples 5.26% (1/19) had S. aureus, in the cooked red meat tantuni samples 1.66% (1/60) had S. aureus, and 3.33% (2/60) yeast-mould which were not compatible with the limit values that were stated at the Turkish Food Codex were found. However, values obtained from this study show that during preparation and production of the goods and in the other stages; hygienic rules have not been carried out.In conclusion to secure the product safety, it is essential to be cautious for the temperature and time during preparation, reservation temperature and GMP/GHP based applications in the preparation of tantuni.
Show more [+] Less [-]Morphometrical analysis of chicken Cryptosporidium on electron microscopy
1999
Park, N.Y. | Kim, Y.S. | Chung, C.Y. | Cho, K.O. | Park, Y.S. | Lee, B.J. | Park, H.S. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Morphometrical analysis of chicken Crytosporidium baileyi in various stages of life cycle in the bursa of Fabricius wer carried out by electron microscope to establish a differential point for identification of C baileyi. By avidin-biotin complex method, protozoans of the bursa of Favricius were identified Cryptosporidium spp. The size and area on each developmental stages of C baileyi, as measured by Morphomat 10 attached to electron microscope were as follews. Trophozoites' size with range of 3.21+_0.70*2.49+_0.59 micro meter, area with range of 118. 82+_41.92 micro meter2; meronts' size with 3.99+_1.07*2.96+_0.52 micro meter, area 210.11+_57.11 micro meter2 ; merozoites' size 1.98+_0.43*0.60+_0.18 micro meter, area 24.10+_5.97 micro meter2; microgametes' size 1.36+_0.83*0.50+_0.23 micro meter, area 20.23+_6.73 micro meter2; macrogametes'size 4.57+_0.65*4.02+_0.55 micro meter, area 258.37+_51.83 micro meter2; oocytes' size 4.39+_0.56*3.44+_0.50 micro meter, area 187.21 +_62.68 micro meter2. In conclusion, the size and area on each developmental stages of Cryptosporidium baileyi is different from that of other Cryptosporidia spp. It suggests, with considering tissue tropism and life cycle, morphometrical analysis can be quite a good way to identify C baileyi.
Show more [+] Less [-]Effect of vacuum packaging on microbiological quality of roasted chicken.
2010
Bharti, Sanjay Kumar | Tanwar, V. K. | Anita, | Palod, J.
The effect of vacuum packaging on microbiological quality of roasted chicken. Roasted chicken were subjected to two different type of packaging treatment i.e. aerobic packaging with low density polyethylene bags (con) and vacuum packaging using barrier bags (VP.). Microbiological analyses were done on 0th, 5th, 10th, 15th and 20th day at refrigeration temperature (4±1oC). Studies revealed that microbial counts in terms of total plate count, proteolytic count and yeast and mold count increased significantly (P0.05) with the advancement of storage period and were significantly higher (P0.05) for aerobically packaged product throughout the observation period however, yeast and mould count observed only on 10th, 15th and 20th day of observation period. Lactic acid bacterial counts of vacuum packaged product were significantly higher as compared to aerobically packaged sample.
Show more [+] Less [-]Isolation of Echinococcus multilocularis from the liver of swine in Hokkaido, Japan
1987
Kamiya, M. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Ooi, H.K. | Oku, Y. | Okamoto, M. | Ohbayashi, M. | Seki, N.
Isolation and numerical taxonomic study of urease-positive aerobic corynebacteria from lower urinary tract of healthy swine
1987
Kudo, Y. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Yanagawa, R.