BACKGROUND: Probiotics have more functional effects and less survival  under hard acidic-bile circumstances of digestive system, and foodstuff products situation has persuaded investigators to find techniques to resolve this problem. Microencapsulation as a useful method has a perceptible effect in this regard. Objectives: The aim of this study was to assess the protective role of double coated beads of calcium alginate-chitosan-eudragit S100 achieved from microencapsulation of Lactobacillus acidophilus as a predominant flora of human and animals gut. Methods: Following activation of starter culture of L.acidophilus in MRS-broth medium,  centrifuge (at aspeed of 5000 rpm for 10 minutes) was used to purify bacteria. Extrusion technique was used for Microencapsulation of probiotic bacterium. The survey of beads solidity was carried out for 12 hours and the study of survival of microencapsulated bacteria was done for 120 minutes inside hydrochloric acid, phosphate buffer and digestive powder solution.  MRS-Salicin-agar and pour plate method and incubation at 37oC for 48 h was done for cultivation. Data were analyzed by means of an independent t-test. Results: Shape and size of beads were shown by optical microscope. The consequences demonstrated that survivability of microencapsulated bacteria in the mentioned conditions, in both situation with and without mechanical tensions, is significantly more than free bacteria (p<0/05). Conclusions: Microencapsulation with calcium alginate- chitosan-eudragit S100 plays a significant role  in increasing the rate of L. acidophilus viability.

BACKGROUND: Microencapsulation of probiotic bacteria is an area that has rapidly expanded over the recent years. It is one of the new methods of improving probiotics stability, through which the biological agents are protected with an enclosed coating to release the active agents within the coating at a controlled rate in time and under special conditions.OBJECTIVES: The present study the aims to evaluate the effect of Lactobacillus plantarum microencapsulation with chitosan/alginate microparticles on the oxidative response of Nile tilapia fish (Oreochromis niloticus).METHODS: Herein, 240 pieces of Nile tilapia, with an average weight of 15.56±0.02 g, were randomly divided into four groups as follows: group1 or control with a diet without probiotics, groups 2, 3, and 4 respectively with a diet containing 108 Log CFU/g of unencapsulated Lactobacillus plantarum, a diet containing microencapsulated Lactobacillus plantarum, and with feed containing alginate with chitosan without the presence of bacteria. They were fed for 60 days at the rate of 2 % of body weight twice a day.RESULTS: The activity level of oxidative enzymes, biochemical factors, and liver enzymes of the Nile tilapia fish were investigated. The findings revealed that oxidative enzymes, biochemical factors, and liver enzymes were positively affected in the groups with microencapsulated Lactobacillus plantarum (P<0.05). The three enzymes of alanine transaminase, aspartate transaminase, and alkaline phosphatase were higher in the micro-encapsulated probiotic group compared to the two groups of probiotics and alginate with chitosan without the presence of bacteria, and all the three groups performed better compared to the control (P<0.05).CONCLUSIONS: Microencapsulation of probiotics maximizes the efficiency of probiotics in reducing oxidative stress.

Objective-To evaluate the efficacy of an orally administered vaccine of Mycoplasma hyopneumoniae that was prepared by spray drying or solvent evaporation. Animals-Thirty 6-week-old, crossbred, specificpathogen- free (SPF) pigs. Procedure-Pigs were randomly allocated into 5 groups and housed in an SPF facility. Pigs in 2 groups (groups AQ and CAP) were fed M hyopneumoniae enteric-coated vaccine on days 0, 10, and 20. A third group (group IM) received an IM injection of M hyopneumoniae vaccine with aluminium hydroxide as an adjuvant on days 0, 10, and 20. The last 2 groups (nonvaccinated- challenged [NV-C] and nonchallenged [NC]) were fed a sham treatment. All 24 pigs in groups AQ, CAP, IM, and NV-C were challenge exposed with 5 ml of a 10% pneumonic lung suspension administered on day 40 via intubation of the trachea. All pigs were slaughtered and the lungs removed and examined for lesions on day 68. Results-In vitro studies indicated that these 2 microencapsulation techniques formed an effective shell and protected mycoplasmal antigen from gastric acid. Results of inoculation and challenge tests indicated that microencapsulated M hyopneumoniae were sufficiently potent to induce an immune response and provide good protection. Conclusions and Clinical Relevance-Orally administered microencapsulated M hyopneumoniae vaccines induced an immune response and reduced the severity of lung lesions in challengeexposed pigs. Results suggest that this novel method can be applied to other antigens, because the spray-drying process yielded an orally administered M hyopneumoniae vaccine that induced a good immune response.

Administration of progesterone in poly(d-,l-lactide) microspheres was used to maintain pregnancy in mares after luteolysis was induced by treatment with pros F2 alpha at day 14 of pregnancy. Mares were given vehicle only (control, n = 6) or 0.75 g (n = 7), 1.5 g (n = 8), or 2.25 g (n = 5) of microencapsulated progesterone at days 12 and 22 of pregnancy. Serum progesterone concentrations were determined daily, and pregnancy was evaluated by transrectal ultrasonography on alternate days. Significantly (P < 0.05) more mares given 1.5 or 2.25 g of progesterone (6 of 8 and 4 of 5 mares, respectively), but not those given 0.75 g (3 of 7 mares), maintained pregnancy through day 32, compared with control mares (O of 6). Progesterone concentrations decreased significantly (P < 0.025) in all groups after administration of prostaglandin F2 alpha at day 14, and significant (P < 0.05) effects of time and treatment on progesterone concentrations were found between days 12 and 22, and 22 and 32. Although treatment with 1.5-g and 2.25-g doses of microencapsulated progesterone improved maintenance of pregnancy, compared with that of vehicle-treated controls, administration of 2.25 g of microencapsulated progesterone appeared to be most efficacious in maintenance of pregnancy during the study interval.

The purpose of this study was to determine the pharmacokinetics of cannabidiol (CBD) in healthy dogs. Thirty, healthy research dogs were assigned to receive 1 of 3 formulations (oral microencapsulated oil beads, oral CBD-infused oil, or CBD-infused transdermal cream), at a dose of 75 mg or 150 mg q12h for 6 wk. Serial cannabidiol plasma concentrations were measured over the first 12 h and repeated at 2, 4, and 6 wk. Higher systemic exposures were observed with the oral CBD-infused oil formulation and the half-life after a 75-mg and 150-mg dose was 199.7 ± 55.9 and 127.5 ± 32.2 min, respectively. Exposure is dose-proportional and the oral CBD-infused oil provides the most favorable pharmacokinetic profile.

Objective: This experiment investigated the effect of microencapsulated turmeric by maltodextrin as an amorphous matrix material on the health status of broiler chickens. Materials and Methods: The broilers used were 144 healthy 1-day-old males. The average body weight was 47.8 ± 1.42 gm. The statistical design was based on a completely randomized design with four treatments and six replications. There were six broiler chickens in each experimental unit. The treatments were TM0 = 0 gm/kg of basal feed, TM1 = 1 gm/kg of basal feed, TM2 = 2 gm/kg of basal feed, and TM3 = 3 gm/kg of basal feed. The growth performance, physical traits, internal organs, microbial population, intestinal morphology, hematological parameters, and anti¬oxidant profile were examined. Results: The results reported that microencapsulated turmeric by maltodextrin as an amorphous matrix significantly improved the hematological parameters, growth performance, antioxidant profile, LAB, immune organs, and intestinal morphology. The results also show decreasing coli¬form and pH of the cecum. Conclusions: Dietary addition of maltodextrin microencapsulated turmeric of 3 gm/kg in basal feed can be used as a natural feed additive to improve the health status of broiler chickens. [J Adv Vet Anim Res 2022; 9(2.000): 221-229]