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Separation of monocytes from canine peripheral blood
1989
Kim, J.B. | Lee, B.W. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Veterinary Medicine)
Pure separation of various leukocytes is required for the assessment of their roles in immunological and physiological function. In this study, pure separation of monocytes from canine peripheral blood was attempted. At first, mononuclear cells (PBMC) were separated by ficoll-hypaque gradient method and then monocytes were recovered from PBMC suspensions in sucrose gradient Sol. (PBMC-Sucrose), autologous plasma (PBMC-Plasma) and autologous serum (PBMC-Serum) incubated at 37deg C for 2 hours. In the separation of PBMC by ficoll-hypaque gradient method in canine blood, higher relative centrifugal force (RCF) was required, as high as more than 1,300xg RCF for 40 minutes, for clear formation of PBMC layer than that in human blood as usually used 400xg RCF for 40 minutes. In monocytes-separation from three PBMC suspensions following PBMC separation, recovery-, purity- and viability- rate of monocytes showed better results in PBMC-Plasma and PBMC-Serum than in PBMC-Sucrose suspension, particularly showing better results from PBMC suspensions performed by centrifugation at 1,500xg RCF for 40 minutes
Show more [+] Less [-]Enhancement of cell-mediated immunity by administration of plasma protein in pigs-(1)-Proportion of leukocyte subpopulations and cells expressing adhesion molecules in peripheral blood
1994
Yang, C.K. (Thaihan Industry Company, Seoul (Korea Republic). Veterinary Diagnostic Laboratory) | Kim, S.J. (Konkuk University, Seoul (Korea Republic). Department of Veterinary Medicine) | Moon, J.S. | Jung, S.C. | Park, Y.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Effect of Euonymus alatus and Ulmus clavidiana var japonica on the immune system
1994
Kim, J.M. | Cho, J.G. | Jung, Y.M. | Park, T.W. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine) | Choi, M.S. (Kunsan National University, Kunsan (Korea Republic). College of Ocean Science and Technology)
Effect of Bupleurum falcatum on the immune system
1994
Cho, J.G. | Kim, J.M. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
