Condensation of 6-methoxy benzothiazol-2-amine with 2-hydroxy- naphthaldehyde gave newSchiff-base derivative in good yield. The metal complex of Co(II) have been synthesized withSchiff base of benzothiazol derivative. Spectroscopic study such as, two dimensional NMR ofnew compound have been obtained by using nuclear magnatic resonance 600 MHz.Computational study of all compounds was calculated using Gaussian 09 program package. Thesynthesized compounds were screened for their antibacterial activity against Staphylococcusaureus, Escherichia coli, Bacillus subtilis, Klebsiella pneumonia. Additionally, the compoundswere tested for antifungal activity against Candida albicans, Candida tropica, Aspergillus multiand Aspergillus niger. Cobalt complex compound exhibited more activity as antifungal thanbenzothiazole derivative.