This study was planned to assess the possible relationships with pregnancy-associated glycoproteins (PAG) concentrations by the determination of the biomarkers of oxidative stress in the plasma of dairy cattle during the early period of gestation. Materials and Methods: Blood samples were collected from coccygeal vessels in pregnant (n = 54) and nonpregnant (n = 45) cows. Measurement of biomarkers of oxidative stress (LPO, GSH and SOD) was carried out in females using spectrophotometric method.Plasma PAG concentrations increased and continuously over the both periods investigated. There were significant differences between pregnant and non-pregnant groups (P < 0.001). The concentration of SOD were significantly lower (P< 0.05) in pregnant females from day 25 to 35 (7.08 ±0.31 U.ml-1) and day 36 to 50 after AI (6.6 ± 0.29 U.ml-1) compared with non-pregnant cows (7.59 ± 0.35 U.ml-1). Concerning the concentrations of LPO and GSH, the values obtained were also significant lower (P < 0.05) in pregnant females in the period 25-35 days post AI (122.7 ±10.27 μM and 6.46 ±1.24 μmol/min.ml-1, respectively) and 36-50 days post AI (108.05±6.17 μM and 6.2±0.77 μmol/min.ml-1, respectively) than in the non-pregnant females (124.8 ± 12.16 μM and 6.96 ± 0.92 μmol/min.ml-1, respectively).It was observed that the markers of oxidative stress tended to be higher in non-pregnant females compared with pregnant females during the early period of gestation in dairy cattle. Our results suggest the existence of a relationship among the concentration of oxidative stress markers and PAG during early pregnancy.

This study aimed to monitor oxidative stress parameters including total antioxidant status (TAS) and total oxidant status (TOS), and tumor necrosis factor-alpha (TNF-α) level during the healing process in hair goats with metritis. This study was carried out on a total of 25 hair goats with metritis (n=10; Group 1) and healthy (n=15; Group 2). The beginning of the study was accepted as day 0. In both Group 1 and Group 2, blood samples were collected on days 0 (first measurement day), 14 (second measurement day) and 28 (third measurement day) of the study for TAS, TOS and TNF-α analyzes. In addition, oxidative stress index (OSI) was calculated using TAS and TOS values.It was found that TAS level decreased in Group 1 compared to Group 2 at the first measurement day (p<0.05). However, TOS, OSI and TNF-α levels increased in Group 1 compared to Group 2 (p<0.01). At the second measurement day, TOS and OSI values were higher in Group 1 than Group 2 (p<0.05).In conclusion, the antioxidant system weakened, oxidative stress and TNF-α levels increased in animals with metritis compared to healthy animals. However, at the third measurement time, all parameters became similar between groups.