Using an avidin-biotin-peroxidase complex immunoperoxidase staining method, respiratory syncytial virus (RSV) antigen was demonstrated in glutaraldehyde-fixed, parraffin-processed lung sections from calves with induced RSV pneumonia. The virus also was detected in formalin-fixed, paraffin-processed lung sections from calves with naturally occurring RSV pneumonia. Specific immunoperoxidase staining was detected within the cytoplasm of epithelial cells and syncytia in small bronchi, bronchioli, and alveoli. Staining also was detected within exudates in airway lumina and in mononuclear and multinucleate cells within alveolar lumina. Optimal intensity of staining was achieved by proteolytic enzyme treatment of lung sections, using 0 .1% pronase and overnight incubation in diluted primary antiserum. The distribution of antigen had a close correlation with presence of lesions. Antigen-staining patterns were similar in lung tissue from calves with naturally occurring and induced RSV disease.

Septicaemic pasteurellosis is a fatal, sometimes epidemic, bacterial disease of domestic and wild animals including deer, bison, elk, and pronghorn antelope caused by Pasteurella multocida. This is the case report of septicaemic pasteurellosisin a captive sambar deer. The carcass was sent from Royal Endurance Stable, Bachok, Kelantan to the Kota Bharu RegionalVeterinary Laboratory for post-mortem. Gross examination of organs was followed by collection of specimens from lung, kidney,liver, spleen and heart for histopathology and bacterial examination. Pooled organ samples with rumen content were collected and sent to the nearest Chemistry Department for investigation. For histology, the liver, lung, spleen, kidney, and heart specimens were fixed in 10% neutral formalin, and routinely embedded in paraffin. Fivemicrometer sections were stained with H&E. Other tests such as worm and ectoparasiteidentification were conducted to identify the parasites. Post-mortem lesions revealed generalised haemorrhage in the organs.Pasteurella multocida serogroup B and E. coli were isolated from multiple tissues of the animal. Histological examination alsorevealed severe congestion and haemorhage of multiple tissues with infiltration of the inflammatory cells. The most likely mode of transmission of these bacteria is through an infected wound and into the bloodstream, thereby causing severe septicemia and death to the animal.

This report describes the parasitological and pathological information retrieved from the samples submitted to Regional Veterinary Laboratory, Bukit Tengah, Penang (MVKBT) followingan episode of histomoniasis in a small scale poultry farm. Liver and ceca from two dead turkey birds, water (n=5) and freshwater snails (Pomacea sp, n=7) were stored between 4°C to 10°C until analyzed. Soil samples (n=7) were preserved in 5% formalin and kept at room temperature prior to further testing. The soil samples were filtered through laboratory test sieves before the filtrates at each level were examined using direct microscopy. Portions of the liver were processed using routine paraffin technique for histopathological examination and found positive for Histomoniasis. Results from direct microscopy of the liver samples revealed Schistosoma mansoni ova, Sarcocystis sporocysts and Fasciola sp. Heterakis sp ova were detected from cecal content. Four out of seven soil samples were positive for Sarcocystis sporocysts, Coccidia oocyst, Strongyles ova and nematod larva. Water and freshwater snail samples were negative for cercaria of Schistosoma mansoni. This case was concluded as Histomoniasis, Sarcocystosis and helminthiasis with incidental finding of Schistosoma mansoni.