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Study on DNase activity specific from Haemonchus contortus reproductive tissue
Kwak, D.M.(Washington State University, Pullman, WA, USA)E-mail:dmkwak@vetmed.wsu.edu
DNase activity in Haemonchus contortus reproductive tissue was characterized and compared to that in whole worm. DNase activity in reproductive tissue was detected throughout pHs 4-10 with high activity under acidic conditions. The activity was not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. The activity produced DNA fragments with mixtures of 3'-hydroxyls (OH) and 3'-phosphates (P) at each pH. Three distinct DNase activities were identified and had M∧rs of 34, 36 and 38.5 kDa in zymograms, which were distinguished according to pH requirement and sensitivity to EDTA.
Show more [+] Less [-]Comparison of DNase activities from excretory/secretory products of Haemonchus contortus fenbendazole-resistant
Kwak, D.M.(Washington State University, Pullman, WA, USA)E-mail:dmkwak@vetmed.wsu.edu
Change in β-tubulin nucleic acid and protein sequences was the only known difference between Haemonchus contortus fenbendazole (FBZ)-resistant and -susceptible isolates. This change was sufficient to determine the pathologic effect induced by FBZ treatment. This research was initiated to investigate further differences from these two isolates. Since β-tubulin is involved in formation of microtubule, which has functions in secretory vesicle transport, DNase activities from excretory/secretory products (ESP) of the two isolates were compared, based on pH, sensitivity to DNase inhibitors, molecular masses and production of 3'-OH.
Show more [+] Less [-]Epizootiological survey of blood parasites in slaughtered cattle of western area of Kyeongnam
1990
Jang, D.H. | Suh, M.D. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
In vitro cultivation from development of Ascaris suum for the decoated and embryonated eggs (second-stage larva)
1998
Jee, C.H. | Park, S.J. (Chungbuk National University, Chongju (Korea Republic). College of Veterinary Medicine)
Studies on the contact infection of canine demodicosis (1)
1992
Lee, C.Y. | Lee, C.H. | Seo, G.W. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Effect of the anti-parasitic activity treated with conventional medicine-acupuncture
1998
Youn, H.J. | Hong, K.O. | Lee, J.M. | Yoon, Y.M. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Noh, J.W. (Shincheongbong Farm, Suwon (Korea Republic)) | Oh, W.G. (Taihan Sugar Industrial Company, Seoul (Korea Republic). Central Research Institute)
In vitro isolation of a bovine Neospora in Korea
1998
Kim, J.H. | Sohn, H.J. | Hwang, E.K. | Jean, Y.H. | Rhee, J.C. | Kang, Y.B. (National Veterinary Research Institute, Anyang (Korea Republic)) | Hwang, W.S. | Hur, K. | Lee, B.C. | Kim, D.Y. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Itsuro, Y. (National Institute of Animal Health, (Japan). Laboratory of Epidemiology)
Project to increase productivity of livestock in Honam area against UR
1994
Lee, J.M. | Kwon, O.D. | Chae, J.S. | Kim, M.C. | Kim, H.S. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine) | Lee, S.J. | Lee, H.S. | Roh, S.I. | Kim, K.S. (Chonbuk Veterinary Service Laboratory, Chonju (Korea Republic))
