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Diagnosis of canine distemper by in situ hybridization
1999
Cho, H. | Park, N.Y. | Kim, Y.H. | Cho, K.O. | Park, H.S. | Park, Y.S. | Lee, B.J. | Chung, C.Y. | Im, H.H. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
We have developed in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues of cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies wer observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.
Show more [+] Less [-]Specific detection of Salmonella serogroup D1 by polymerase chain reaction(PCR) for sefA gene
1999
Jun, M.H. | Kim, T.J. | Chang, K.S. | Kang, K.I. | Kim, K.H. | Kim, H.S. | Shin, K.S. | Kim, C.J. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine) | Kim, K.S. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).) | Yoo, S.S. (Taejon City Institute of Health and Environment, Taejon (Korea Republic).)
Sal enteritidis thin fimbriae, SEF14, were found to be restricted to the predominantly poultry-associated members of the Salmonella serogroup D1 that are considered as the important pathogens in poultry industry. SefA together with sefB and sdfC encode the proteins involved in SEF14 biosynthesis. In order to develop the rapid and specific detection methods for Salmonella serogroup D1, a PCR technique for the am;lification of sefA gene was established, and its specificity and sensitivity were investigated with various microorganisms. The bacterial genomic DNA was extracted by colony-picking and rapid boiled-lysate technique. In comparison of SefI and SefII primers used in the PCR. SefI primer for sefA gene of 513bp showed higher specificity than that of SefII. The established PCR was s sensitive as to detect 1pg of Sal enteritidis DNA. When 73 strains in 28 genera including the reference strains and the field isolates of various Salmonella serotypes, Bacillus subtilis, Bordetella bronchiseptica, E coli, Listeria spp., Micrococcus luteus, Rhodococcus equi, Staphylococcus spp., Streptococcus spp., Vibrio parahemolyticus, Yersinia spp. were studied, the established PCR yielded specifically positive results with only Salmonella serogroup D1. The results suggested that the PCR for sefA gene could be a potential candidate among the specific detection methods for Salmonella serogroup D1.
Show more [+] Less [-]Antibacterial efficacy and safety of copper sulfate pentahydrate to cultured fish
1997
Heo, G.J. (Chungbuk National University, Chongju (Korea Republic). College of Veterinary Medicine)
Elimination of respiratory pathogens in endemically infected swine herds by nursery depopulation
1997
Kim, B.H. (Kyongpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Joo, H.S. (University of Minnesota, Minnesota (USA). College of Veterinary Medicine)
Relationship between the N-acetyl-Beta-D-glucosarminidase levels and the presence of mastitis pathogens in bovine mastitis milk samples
1993
Kang, B.K. | Nam, H.M. | Shon, C.H. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)