The objective of the study was to establish the baseline values for blood and coagulation parameters in normal and healthy rusa deer (Rusa timorensis) of different ages and sexes. The samplepopulation consists of 40 rusa deer, divided into four groups of (i) juvenile males (ii) juvenile females (iii) adult males and (iv) adult females. The findings showed significant (p<0.05) higher values in erythrocyte count, calcium concentration and prothrombin time in the adult males compared to adult female rusa deer. On the other hand, the total protein concentration was significantly higher in adult females than adult male deer. No significant differences in blood or coagulation parameters were observed between sexes in the juvenile deer. Between age group, the adult deer had significantly higher mean cell volume, plasma protein and globulin concentration than juvenile rusa deer. Thus, it is necessary to take into account the age and sex of the rusa deer when using blood reference values for the diagnosis of diseases or health assessment.

Identification of processed animal protein in animal feedstuffs wasperformed under the feed safety programme to ensure that the products used locally to feed the livestock are safe and properly labeled to prevent unnecessary incidence that will affect both animal and human.A “silica-membrane technology” method was applied based on its fast and effective purification of nucleic acids from various matrices. The silica membranes were optimized for high DNA recovery and lowbinding efficiency for impurities. Results from 58 with various kinds of samples showed negative of unwanted processed animal protein.

Information on the nutritional content of animal feed is very important in improving milk production and the quality of milk produced. Data on the nutrition and nutritional quality of feed given to livestock are not comprehensively evaluated and recorded in any dairy farms in Johore. The objective of this study is to determine the nutritional content of selected feedstuffs, with production and quality of milk in selected dairy farms in Johore. Data were collected from 94 dairy farms during field visits through face-toface interviews. Feed samples collected were dairy cattle pellet, Napier grass, palm kernel cake, palm kernel expeller, soybean hull, corn, cassava peel and agricultural waste. Proximate analysis was performed using standard AOAC methods. The result of this study found that 67.0% of dairy farmers in Johore use Napier grass as the main source of fodder. The survey showed that 46% of the farmers in Johore give a suitable quantity of Napier grass based on body weight, while for milk production, the survey showed that 54 dairy farms in Johore produce less than 100 litres/day, 18 farms produce 101-200 litres/day, 12 farms produce 201-300 litres/day, and 10 farms produce more than 300 litres/day. The overall status of dairy farms in Johore shows that 60% of the farms produce an average of 5.1-10 litre milk per cow per day in which 92.5% of milk samples collected had acceptable Total Dissolved Solid values indicating good quality of milk production.

Objective: This study investigated the effectiveness of different physical forms of feed containing Ipomoea aquatica waste and concentrate feed on the rumen characteristics, chewing activity, and performance of sheep. Materials and Methods: Twenty-four rams (19.87 ± 2.19 kg) were arranged in a completely randomized design. Rams were fed dried I. aquatica waste and concentrate feed provided separately (RCF) (conventional feeding system), and total mixed ration consisted of mash complete feed (MCF), and pelleted complete feed (PCF). The data were analyzed using a one-way analysis of variance. Results: The dry matter intake (DMI), average daily gain, and feed conversion ratio of rams fed different physical forms of feed containing I. aquatica waste in their diet were similar between the treatments, ranging from 4.08% to 4.29% of body weight, 120–180 gm, and 6.32–9.17, respectively. Different physical feeds did not affect microbial synthesis in the rumen. Methane emissions per unit of production were similar between the groups. The PCF sheep ate faster (0.24 min/gm DMI) than the MCF sheep (0.38 min/gm DMI), but similar to the RCF sheep (0.31 min/gm DMI). Conclusion: Dried I. aquatica waste was useful as an alternative lamb feed roughage during the dry season. The pelleted mixed ration was more efficient than mash in increasing DMI. The dried I. aquatica waste was environmentally friendly for mitigating enteric methane emissions by sheep. [J Adv Vet Anim Res 2023; 10(4.000): 677-684]