Ceftizoxime is the third generation cephalosporin, extensively used in veterinary medicine. It has larger volume of distribution, good penetration into tissues, and useful for the treatment of mastitis which is resistant to other antibiotics. Keeping in view of generating pharmacokinetic data of ceftizoxime in different domestic animals, the present study was planned to investigate pharmacokinetics of ceftizoxime in goats (n = 6) at the dose rate of 10mg kg-1 body weight following single dose intravenous administration. Drug concentration in plasma was determined using High Performance Liquid Chromatography (HPLC) with UV detector. The values of Cmax, AUC and MRT were 44.40 ± 2.09 µg ml-1, 121.22 ± 15.40µg.h.ml-1 and 11.44 ± 1.30 h, respectively. The longer elimination half-life (8.92 ± 1.11h) along with smaller ClB (0.09 ± 0.01 Lh-1 kg-1) showed slower excretion of the drug from animal body.