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In vitro testing of a potential retroviral vector for producing transgenic livestock.
1989
Squire K.R.E. | Embretson J.E. | First N.L.
The amphotropic murine leukemia virus (MLV) has been shown to infect mammalian species other than mice. If this virus infects and expresses genes in cells of livestock species (cattle, sheep, and pigs) it has potential for use as a vector to produce transgenic livestock. Because the gene-injection technique for producing transgenic animals is inherently inefficient, our laboratory was interested in identifying or constructing retroviral vectors capable of infecting livestock embryos. The infectivity of an amphotropic MLV-based vector for ovine, bovine, and porcine cells was tested. Experiments were also conducted to test the ability of the amphotropic MLV promoter, compared with known strong promoters, to express genes in cells from these species. Results indicated that amphotropic MLV infects and expresses genes efficiently in porcine cells and is, therefore, a potential vector for producing transgenic pigs. Infection was not detected in cells from adult bovine and ovine species; however, low levels of infection, with subsequent gene expression, were detected in cells derived from bovine embryos.
Show more [+] Less [-]Progression of aflatoxicosis in growing barrows.
1988
Harvey R.B. | Huff W.E. | Kubena L.F. | Corrier D.E. | Phillips T.D.
A scanning electron microscopic study on the architecture of lymph vessels and intranodal lymph pathways of lymph nodes in pigs.
1988
Hoshi N. | Hashimoto Y. | Kitagawa H. | Kon Y. | Kudo N.
In vitro characterization of porcine juvenile articular cartilage.
1987
Lamar C.H. | Eller L.L. | Turek J.J.
Neutralization of African swine fever virus by sera from African swine fever-resistant pigs.
1986
Ruiz Gonzalvo F. | Caballero C. | Martinez J. | Carnero M.E.
Antiviral effectiveness of butylated hydroxytoluene against pseudorabies (Aujeszky's disease) virus in cell culture, mice, and swine.
1986
Pirtle E.C. | Sacks J.M. | Nachman R.J.
Alveolar phospholipids of 17-day-old pigs exposed to microorganisms of nonpulmonic origin.
1986
Engen R.L. | Whipp S. | Hummel S.K.
Iron and transferrin in acute experimental Salmonella cholerae-suis infection in pigs.
1985
Kramer T.T. | Griffith R.W. | Saucke L.
Intradermal testing of swine to monitor changes in delayed hypersensitivity response.
1985
Mann D. | Hargis J.W.
Binding characteristics of swine erythrocyte insulin receptors.
1985
Dieberg G. | Bryan G.S. | Sartin J.L. | Williams J.C. | Prince T.J. | Kemppainen R.J.
