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Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR
2011
Kim, H.K., Seoul National University, Seoul, Republic of Korea | Moon, H.J., Green Cross Veterinary Products, Yongin, Republic of Korea | Park, S.J., Seoul National University, Seoul, Republic of Korea | Rho, S.M., Seoul National University, Seoul, Republic of Korea | Han, J.Y., Seoul National University, Seoul, Republic of Korea | Nguyen, Van Giap, Seoul National University, Seoul, Republic of Korea | Park, B.K., Seoul National University, Seoul, Republic of Korea
Swine diseases could be caused by unrecognized or minor pathogens. In this study, two unknown cytopathogenic agents were isolated from swine, through cell culture. In order to identify these two cytopathogenic agent (designated CP129 and No.2045-7), a particle associated nucleic acids PCR (PAN-PCR) from previous paper was used with simple modification. The cloning procedure was more specified in this study by adding cell control system. According to the modified PAN-PCR, two and four agents-specific DNA sequences were obtained from CP129 and No.2045-7, respectively, and they were identified as Mycoplasma (M.) hyorhinis and Mammalian orthoreovirus by nucleotide BLAST. Since M. hyorhinis (CP129) was filterable and non-visible by microscope, this unusual virus-like nature of M. hyorhinis (CP129) was discussed. Especially, the reovirus (No.2045-7) was a serotype 3 and a triple reassortant among three serotypes of reoviruses. It was grouped with recently reported reoviruses from disease cases (swine, human and feline), based on the genetic analysis of L1 and S1 partial sequences. In conclusion, two unknown cytopathogenic agents were successfully identified using modified PAN-PCR with cell control system and they were characterized in this study.
Show more [+] Less [-]Detection of InvA gene in isolated Salmonella isolates from spleen of pigs by Polymerase Chain Reaction (PCR).
2011
Chopade, N.A | Kurkure, N. V. | Narkhede, H. P. | Barbuddhe, S. B. | Bhandarkar, A.G. | Kalorey, D.R.
Nine Salmonella isolates (11.25%) from 80 spleen samples of pigs collected from slaughter house detected by conventional culturing when subjected to Salmonella-specific gene (invA) yielded product a 284-bp DNA fragment.
Show more [+] Less [-]Prediction of body weight based on body measurements of pig.
2011
Kumar, Ravindra | Kuma, Ashok | Patel, M. | Kumar, Anil | Yadav, A. K.
Atotal of twenty four LargeWhite Yorkshireweaners nearly of same body weight were selected for the present study.The prediction equations were constructed to estimate the body weight of pigs indirectly from different body measurements of 24 pigs. The body weight was highly correlated (PO.OI)with body length, heart girth and height at withers. Accuracy for the measurement of body weight with prediction equation using one or two independent variables shifting from heart girth to height at withers with increase in body weight. Coefficient of multiple determination (R2)increases gradually as the number of independent variables increased in the prediction equation and the best prediction equation was observed when all the 3 body measurements were included in the equation. orrelationcoefficientof body weightwith body measurementswere also foundto be highly correlated.
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