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Bacterial strains isolated from Jeotgal (salted seafood) induce maturation and cytokine production in mouse bone marrow-derived dendritic cells
2014
Moon, S.Y., eju National University, Jeju, Republic of Korea | Park, E.J., eju National University, Jeju, Republic of Korea | Joo, H.G., eju National University, Jeju, Republic of Korea
Jeotgal (salted seafood) has been one of major fermented foods in Korea for long time. Although there are many studies about Jeotgal in various aspects of food, its immunological importance on hosts has not been elucidated yet. In this study, we investigated if several bacteria isolated from Jeotgal may modulate the function of dendritic cells (DCs), powerful antigen-presenting cells equipped with special immunological capabilities. 4 Jeotgal bacteria were selected as representatives and used for experiments. To treat viable DCs, those bacteria were killed at 60¡� for 30 min. The viability of DCs treated with Jeotgal bacteria was verified and two isolates significantly induced high production of interleukin-12, a representative cell-mediated cytokine of DCs. Surface activation and maturation markers (MHC class II, CD40, CD86) of DCs were analyzed by flow cytometer. In addition, the treated DCs showed significantly high lymphocyte stimulatory capability compared to control DCs based on allogeneic mixed lymphocyte reactions. These observations suggest that Jeotgal isolates can function as immunostimulating bacteria in hosts, like Lactobacillus. Taken together, these experimental evidences may broaden the use of Jeotgal isolates in immunological fields in addition to as a fermented food.
Show more [+] Less [-]Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk
2012
Kweon, C.H., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Choi, S.Y., Sungkyunkwan University, Suwon, Republic of Korea | Kwon, H.Y., Sungkyunkwan University, Suwon, Republic of Korea | Kim, E.H., Sungkyunkwan University, Suwon, Republic of Korea | Kang, H.M., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Moon, J.S., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Jang, G.C., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, H.S., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kang, S.W., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kim, J.M., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Pyo, S.N., Sungkyunkwan University, Suwon, Republic of Korea | Rhee, D.K., Sungkyunkwan University, Suwon, Republic of Korea
Probiotics colonize the intestines and exert an antibacterial effect on pathogens. Therefore, probiotics could be used as a preventive agent against lethal infections. To isolate probiotic microorganisms, 116 bacterial strains were isolated from healthy cow's milk and were subjected to Gram-stain, morphology and biochemical analyses, Vitek analysis, and 16S rRNA analysis. One of the strains identified as Bacillus (B.) thuringiensis 87 was found to grow very well at pH 4.0~7.0 and to be resistant to high concentrations of bile salts (0.3~0.9% w/v). B. thuringiensis was susceptible to the antibiotics used in the treatment of bovine mastitis, yet it exhibited an antimicrobial effect against Staphylococcus (S.) aureus 305. Moreover, it protected mice from experimental lethal infections of E. coli O55, Salmonella typhimurium 01D, and S. aureus 305 through a significant induction of interferon-γ, even at four-week post-administration of B. thuringiensis. Although oral administration of B. thuringiensis 87 did not provide significant protection against these lethal challenges, these results suggest that B. thuringiensis 87 could be a feasible candidate as a probiotic strain.
Show more [+] Less [-]In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets
2008
Oh, Y.R. (Konkuk University, Seoul, Republic of Korea) | Lee, J.B. (Konkuk University, Seoul, Republic of Korea) | Park, S.Y. (Konkuk University, Seoul, Republic of Korea) | Song, C.S. (Konkuk University, Seoul, Republic of Korea) | Choi, I.S. (Konkuk University, Seoul, Republic of Korea) | Kim, Y.H. (Bio Medic Support, Namyangju, Republic of Korea) | Han, E.J. (Korea Yakult Co., Yongin, Republic of Korea) | Lee, J.H. (Korea Yakult Co., Yongin, Republic of Korea) | Lim, K.S. (Korea Yakult Co., Yongin, Republic of Korea) | Huh, C.S. (Korea Yakult Co., Yongin, Republic of Korea) | Kim, S.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Park, S.S. (Seoul National University, Seoul, Republic of Korea) | Lee, S.W. (Konkuk University, Seoul, Republic of Korea), E-mail: odssey@hanmail.net
Lactic acid bacteria have been reported their beneficial roles on host including reduction of infectious diarrhea problems. In this study, preventive effect of Lactobacillus (L.) reuteri HY25101 and L. johnsonii HY25103 on porcine epidemic diarrhea virus (PEDV) was investigated in suckling piglets. Two groups of one day old PEDV naive piglets were orally administered L. reuteri HY25101 and L. johnsonii HY25103 for three days respectively before challenge with lethal dose of PEDV. In second experiment, passive immunized one day old piglets using colostrums containing PEDV specific IgA were used. The survival rates of the L. reuteri HY25101 administered group were significantly higher than that of L. johnsonii HY25103 administered group and viral shedding was rapidly diminished in L. reuteri HY25101 administered group. Interestingly piglets born from the sow immunized with attenuated PEDV vaccine were not completely protected from PEDV challenge, however coadministeration of L. reuteri HY25101 and colostrums containing PEDV specific IgA were more effectively prevent PEDV infection. These results suggested that dietary treatment using L. reuteri HY25101 could reduce diarrheal problem and mortality rate caused by PEDV in suckling pigs. In addition, L. reuteri HY25101 could be used as one of effective compensation treatment with attenuated live vaccine for PED.
Show more [+] Less [-]Properties of Enterococcus faecium isolated from the intestine of slaughting pigs
Kim, I.D.;Ahn, M.H.;Seok, H.B.(Dankook University, Cheonan, Republic of Korea)E-mail:hobong@dankook.ac.kr
The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of 1×10∨7 CFU/ml~3.3×10∨9 CFU/ml in viable bacterial count on MRI medium.
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