A primary fibroblast cells from embryos of brown quail Coturnixypsilophora has been established and partially characterized. The cells were maintained in Modified Eagle’s medium (MEM) supplemented with 10% fetal bovine serum. The cells were able to grow at temperatures between 35°C and 38°C with optimum temperature of 37°C. The growth rate of primary quail fibroblast cells increased as the FBS proportion increased from 5% to 20% at 37°C with optimum growth at the concentrations of 10% or 15% FBS. The cells showed no microbial contamination throughout the period of experiment and the total chromosome number of a diploid cell was 78, according to karyotyping and chromosome analysis. The susceptibility of quail primary cells for avian viruses was investigated in this study after inoculation with ND and IB viruses. Both viruses showed a satisfactory CPE development and the infectivity were assayed by virus titration (TCID50). This suggests that the quail primary cells can be used for isolation of various avian viruses with further steps of infectivity confirmation in the future.

Quail closed-house system is a house that support quails’ optimumrequirements as far as temperature, relative humidity, ventilation and light are concerned. One of the four units of quail closed-house systems at the Institute of Poultry Technology, Malacca was used in this study. The objective of this project was to evaluate the performance of the quail closed-house system by comparing the condition of the house under current management condition and after the Standard Operating Procedure determined by DVS was adhered. Thehouse performance was evaluated by observing its ability to achieve an optimum temperature range of 20°C to 27°C with a relative humidity between 60% to 80% as perfect surroundings for quails. At the end of the experiments, the results showed a decline in internal temperature at almost 3°C and increasing in a relative humidity of 10% after all the specifications and procedures were followed.