Non-human primate (NHP) research faces challenges due to zoonosis risk and complex veterinary management yet lacks standardized guidelines for animal care. Therefore, we developed an advanced veterinary management protocol for NHP quarantine, anesthesia, and postoperative care. Three female 4 to 5-year-old cynomolgus monkeys were anesthetized and underwent various tests, including body weight, temperature, blood tests, urinalysis, microbiological monitoring, and physical and dental examinations. Ivermectin and medicated baths were administered to eradicate ectoparasites and endoparasites, and testing was repeated 30 days later. Following quarantine, we performed computed tomography and anesthesia maintenance for mastoidectomy. To relieve pain and maintain body weight, we administered tramadol intramuscularly 4 times/day for 3 days and meloxicam subcutaneously twice daily for 14 days. Feed replacements were provided. During the 33-day quarantine period, physical examinations revealed no abnormalities indicative of infectious diseases, and no specific clinical symptoms were observed. Through a preliminary test of anesthesia time, we selected ketamine 4 mg/kg + medetomidine 50 µg/kg for short experiments such as computed tomography, and ketamine 8 mg/ kg + medetomidine 50 µg/kg for intubation. Ten days after mastoidectomy, NHPs consumed 100 kcal/kg and recovered their body weight. This study offers advanced veterinary management guideline for NHP research. Such protocols can lead to more standardized and ethical practices in NHP research, thereby enhancing the quality of studies on NHPs and the translation of findings to human health and disease.

The efficacy of a piglet-specific inactivated Porcine circovirus type 2 (PCV2) vaccine was evaluated with clinical field trials, as recommended by the Republic of Korea’s Animal, Plant & Fisheries Quarantine & Inspection Agency. Three farms were selected on the basis of their history of postweaning multisystemic wasting syndrome. On each farm 60, 1-week-old pigs were randomly allocated to 1 of 2 treatment groups: vaccination at 1 and 3 wk of age or no vaccination. The 2-dose schedule of vaccination with inactivated PCV2 vaccine improved the average daily weight gain from birth to 16 wk of age, the PCV2 load in the blood, and the frequency and severity of lymph node lesions. Inactivated PCV2 vaccine seems to be very effective in controlling PCV2 infection under field conditions.

Objective-To evaluate postexposure prophylaxis (PEP) in dogs experimentally infected with rabies. Procedure-29 Beagles. Procedure-Dogs were sedated and inoculated in the right masseter muscle with a salivary gland homogenate from a naturally infected rabid dog (day 0). Six hours later, 5 dogs were treated by administration of 2 murine anti-rabies glycoprotein monoclonal antibodies (mAb) and commercial vaccine; 5 received mAb alone; 5 received purified, heat-treated, equine rabies immune globulin (PHT-ERIG) and vaccine; 5 received PHT-ERIG alone; 4 received vaccine alone; and 5 control dogs were not treated. The mAb or PHTERIG was administered at the site of rabies virus inoculation. Additional vaccine doses for groups mAb plus vaccine, PHT-ERIG plus vaccine, and vaccine alone were administered IM in the right hind limb on days 3, 7, 14, and 35. Results-All control dogs and dogs that received only vaccine developed rabies. In the PHT-ERIG and vaccine group, 2 of 5 dogs were protected, whereas none were protected with PHT-ERIG alone. Use of mAb alone resulted in protection in 4 of 5 dogs. Administration of mAb in combination with vaccine provided protection in all 5 dogs. Conclusions and Clinical Relevance-Current national guidelines recommend euthanasia or a 6- month quarantine for unvaccinated animals exposed to rabies. Findings from this study document that vaccine alone following severe exposure was unable to provide protection from rabies. However, vaccine combined with mAb resulted in protection in all treated dogs, revealing the potential use of mAb in PEP against rabies in naïve dogs.

Temporary quarantine stations (TQS) are transitory premises that havebeen approved to facilitate the quarantine of imported live animals in Malaysia. These stations must abide to the standard operating procedures (SOP) for animal quarantine as outlined by the veterinaryauthority in Malaysia. However, the level of awareness for the quarantine procedures among the TQS operators and managers has not been assessed. This study was conducted to describe thedistribution of the TQS in 2012-2013 and the level of awareness among its operators on the quarantine procedures and the fundamental requirements for quarantine establishments. Eight TQS from 25 wereselected and operators or managers were interviewed using a questionnaire and the facility was visited. The study found thatmajority (82.5%) of the TQS operators were aware of the quarantine procedures but the auditors from the veterinary authorityrevealed vice versa.

Objective: To determine the influence of transportation by road and air on heart rate (HR) and HR variability (HRV) in horses. Animals: 6 healthy horses. Procedures: ECG recordings were obtained from horses before (quarantine with stall rest [Q]; 24 hours) and during a journey that included transportation by road (RT; 4.5 hours), waiting on the ground in an air stall (W; 5.5 hours), and transportation by air (AT; 11 hours); HR was determined, and HRV indices of autonomic nervous activity (low-frequency [LF; 0.01 to 0.07 Hz] and high-frequency [HF; 0.07 to 0.6 Hz] power) were calculated. Results: Mean ± SD HRs during Q, RT, W, and AT were 38.9 ± 1.5 beats/min, 41.7 ± 5.6 beats/min, 41.5 ± 4.3 beats/min, and 48.8 ± 5.6 beats/min, respectively; HR during AT was significantly higher than HR during Q. The LF power was significantly higher during Q (3,454 ± 1,087 milliseconds2) and AT (3,101 ± 567 milliseconds2) than it was during RT (1,824 ± 432 milliseconds2) and W (2,072 ± 616 milliseconds2). During Q, RT, W, and AT, neither HF powers (range, 509 to 927 milliseconds2) nor LF:HF ratios (range, 4.1 to 6.2) differed significantly. The HR during RT was highly correlated with LF power (R2 = 0.979), and HR during AT was moderately correlated with the LF:HF ratio (R2 = 0.477). Conclusions and Clinical Relevance: In horses, HR and HRV indices during RT and AT differed, suggesting that exposure to different stressors results in different autonomic nervous influences on HR.

The objective of this study was to determine the seroprevalence of dourine from imported horses using Complement Fixation Test. Dourine is classified as a OIE listed B disease (OIE Terrestrial Manual 2008) where equines are mainly susceptible to it and it had occurred in many countries such as Africa, the Middle East, South America and South-eastern Europe. Malaysia regularly imports Australian horses for the equestrian sector which encompasses activities such as racing and endurance as well as other recreational or leisure activities in Malaysia. As such, awareness towards this disease is important during importation of horses to avoid dourine embark into Malaysia. A total of 288 horse sera samples obtained from animal quarantine stations and private stables were examined for dourine by complement fixation test. Results showed serologically negative results for dourine with all the samples tested.

Objective: The objective of this study was to examine the newly arrived pure breed sheep considering antibody against some diseases and immunization of the animal against endemic diseases of hosting area according to species. Materials and methods: A total of 42 pure exotic breed of sheep, originated from Australia namely Parendale (n=14), Suffolk (n=13) and Dorper (n=15), imported by the Bangladesh Livestock Research Institute (BLRI) were used in this study. Before shifting the animals to sheep farm, 1-month quarantine was done in an isolated shed by following step by step technique; first week was used for close observation, second week for immunization of animal against endemic diseases, third week for monitoring and observation against immunization, and the fourth week was used for determination of animal health status and immunity level. On day 1 and 2, the sheep remained at full rest along with supplied habituated feed, saline and drinking water. On day 3, fecal sample was collected from all animals to measure parasitic load. On day 4, blood and oculo-nasal discharge samples were collected to detect blood protozoa, peste des petits ruminants (PPR), foot and mouth disease (FMD) and other infectious zoonotic diseases like anthrax, brucellosis and tuberculosis. In the second week, immunization was done against PPR, FMD and hemorrhagic septisemia (HS) at two days interval. Farm biosecurity and biosafety for the workers and officers was maintained according to standard guideline. In the third week, the animals continued to be under close observation. On day 29, the antibody level produced due to vaccination was measured. Results: Parasitic load was found to be very low, and blood protozoa, PPR, FMD, anthrax, brucellosis and tuberculosis were negative. The antibody titer was detected at the acceptable level in sheep on day 29. The quarantine committee decided that the sheep were ready to transfer to the farm along with some recommendations. After completion of 1-month quarantine period, all sheep could not transmit any diseases in Bangladesh. Conclusion: Quarantine is mandatory for entering any new animal in a new area that gives the outline of different new diseases. It also acts as radar of exotic disease in a country. So, this quarantine technique serves the purpose for livestock owners effectively. [J Adv Vet Anim Res 2018; 5(1.000): 67-72]