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Use of aVR and Jastrzębski algorithms in the classification of wide complex tachycardia in dogs – a preliminary study Full text
2018
Michałek, Marcin | Frydrychowski, Piotr | Adamowicz, Jakub | Sławuta, Agnieszka | Pasławska, Urszula | Noszczyk-Nowak, Agnieszka
Use of aVR and Jastrzębski algorithms in the classification of wide complex tachycardia in dogs – a preliminary study Full text
2018
Michałek, Marcin | Frydrychowski, Piotr | Adamowicz, Jakub | Sławuta, Agnieszka | Pasławska, Urszula | Noszczyk-Nowak, Agnieszka
Ventricular rhythm disturbances are a common pathology in human and veterinary medicine. In humans, the algorithmic approach is used to differentiate wide QRS complex tachycardia. The most commonly used are the aVR and Brugada algorithms as well as the ventricular tachycardia (VT) score developed by Jastrzębski and coworkers. In veterinary medicine, no such algorithms are available and the only parameter used to describe VT abnormalities is the duration of the QRS complexes. The aim of this analysis was determining whether human medicine algorithms for VT are applicable in veterinary medicine to differentiate wide QRS complex tachycardia in dogs. A retrospective analysis was performed on 11 dogs of both sexes and various breeds and age diagnosed with VT. The diagnosis was based on ambulatory ECG, further established based on the reaction to lidocaine or adenosine or an invasive electrophysiological study. Of the 11 tracings passed through the aVR algorithm, 10 met the VT criteria. The most common criterion was the Vi/Vt ratio (8 out of 11 tracings). Based on the VT score, seven out of eight dogs had a high probability of VT. Retrospective analysis of ECGs by aVR and VT score indicates that the applied algorithms may be useful in differentiating wide QRS complex tachycardia as a quick, easy, and non-invasive alternative to cardiac electrophysiology.
Show more [+] Less [-]Use of aVR and Jastrzębski algorithms in the classification of wide complex tachycardia in dogs – a preliminary study Full text
2018
Michałek Marcin | Frydrychowski Piotr | Adamowicz Jakub | Sławuta Agnieszka | Pasławska Urszula | Noszczyk-Nowak Agnieszka
Ventricular rhythm disturbances are a common pathology in human and veterinary medicine. In humans, the algorithmic approach is used to differentiate wide QRS complex tachycardia. The most commonly used are the aVR and Brugada algorithms as well as the ventricular tachycardia (VT) score developed by Jastrzębski and coworkers. In veterinary medicine, no such algorithms are available and the only parameter used to describe VT abnormalities is the duration of the QRS complexes. The aim of this analysis was determining whether human medicine algorithms for VT are applicable in veterinary medicine to differentiate wide QRS complex tachycardia in dogs.
Show more [+] Less [-]Prevalence of antibodies to Aujeszky’s disease virus in wild boar in Poland, between 2011 and 2014: a retrospective study Full text
2017
Lipowski, Andrzej | Szczotka-Bochniarz, Anna | Pejsak, Zygmunt
Prevalence of antibodies to Aujeszky’s disease virus in wild boar in Poland, between 2011 and 2014: a retrospective study Full text
2017
Lipowski, Andrzej | Szczotka-Bochniarz, Anna | Pejsak, Zygmunt
Introduction: Aujeszky’s disease virus (ADV) infects a wide range of animals, including members of the Suidae family, i.e. domestic and wild pigs, as well as wild boar. Since wild boar are a potential ADV reservoir and a source of infection for domestic pigs, the aim of the study was to evaluate ADV antibody prevalence in the Polish wild boar population, during the years 2011 to 2014. Material and Methods: Wild boar blood samples were collected during three consecutive hunting seasons; i.e. 2011/2012, 2012/2013, and 2013/2014, and tested for ADV antibodies by ELISA. Results: ADV antibodies were detected in samples from all tested voivodships. The average seroprevalence reached 32.2%. Seroprevalence, over the examined hunting seasons, was 27.4% in 2011/2012, 32.4% in 2012/2013, and 35.5% in 2013/2014. The highest percentage of seroreagents was detected in four voivodships, situated along the western border of Poland, i.e. Zachodnio-Pomorskie (ZP), Lubuskie (LB), Dolnośląskie (DS), and Opolskie (OP). This area is positively correlated with the highest density of the wild boar population and the highest wild boar hunting bag. Conclusion: The results of this study confirm that the wild boar population may still pose a threat to domestic pigs, which is of special importance at the final stage of Aujeszky’s disease eradication programme in Poland.
Show more [+] Less [-]Prevalence of antibodies to Aujeszky’s disease virus in wild boar in Poland, between 2011 and 2014: a retrospective study Full text
2017
Lipowski Andrzej | Szczotka-Bochniarz Anna | Pejsak Zygmunt
Introduction: Aujeszky’s disease virus (ADV) infects a wide range of animals, including members of the Suidae family, i.e. domestic and wild pigs, as well as wild boar. Since wild boar are a potential ADV reservoir and a source of infection for domestic pigs, the aim of the study was to evaluate ADV antibody prevalence in the Polish wild boar population, during the years 2011 to 2014.
Show more [+] Less [-]Epidemiological and histopathological analysis of 40 apocrine sweat gland carcinomas in dogs: a retrospective study Full text
2016
Kycko, Anna | Jasik, Agnieszka | Bocian, Łukasz | Otrocka-Domagała, Iwona | Mikiewicz, Mateusz | Smiech, Anna | Łopuszyński, Wojciech | Dolka, Izabella | Nowak, Marcin | Madej, Janusz A.
Epidemiological and histopathological analysis of 40 apocrine sweat gland carcinomas in dogs: a retrospective study Full text
2016
Kycko, Anna | Jasik, Agnieszka | Bocian, Łukasz | Otrocka-Domagała, Iwona | Mikiewicz, Mateusz | Smiech, Anna | Łopuszyński, Wojciech | Dolka, Izabella | Nowak, Marcin | Madej, Janusz A.
Introduction: Apocrine sweat gland carcinomas (ASGCs) are malignant neoplasms of dogs and other animals, rarely reported worldwide. The aim of this study was to summarise the occurrence of this cancer in a population of dogs in Poland between 2009 and 2014 with regards to histological features and body location of the tumours, as well as age, sex and breed of the cancer-affected dogs.Material and Methods: The study involved 40 canine ASGC cases diagnosed in five national veterinary pathology laboratories. The material was processed according to routine histological methods.Results: Histological types of the tumours involved simple and complex apocrine carcinoma of cystic/papillary (62.5%), solid (15%), and tubular type (12.5%), as well as apocrine ductal carcinoma (10%). The epidemiological analysis revealed peak incidence of the cancer in dogs between 8 and 14 years of age, with the most commonly affected sites being forelimbs and thorax. The highest number of the cancer cases was diagnosed in mixed breed dogs and German Shepherds; no sex predilection was noted.Conclusion: To the authors’ knowledge, this is the first report recounting the study on canine malignant apocrine sweat gland tumours in Poland providing detailed phenotypical and histological data, which are otherwise rarely described in veterinary literature. This type of cancer appears to be diagnosed more frequently in dogs than in humans. Being an easily accessible material for research, canine ASGCs might serve as a relevant animal model for studies related to pathogenesis of sweat gland tumours.
Show more [+] Less [-]Epidemiological and histopathological analysis of 40 apocrine sweat gland carcinomas in dogs: a retrospective study Full text
2016
Kycko Anna | Jasik Agnieszka | Bocian Łukasz | Otrocka-Domagała Iwona | Mikiewicz Mateusz | Śmiech Anna | Łopuszyński Wojciech | Dolka Izabella | Nowak Marcin | Madej Janusz A.
Introduction: Apocrine sweat gland carcinomas (ASGCs) are malignant neoplasms of dogs and other animals, rarely reported worldwide. The aim of this study was to summarise the occurrence of this cancer in a population of dogs in Poland between 2009 and 2014 with regards to histological features and body location of the tumours, as well as age, sex and breed of the cancer-affected dogs.
Show more [+] Less [-]Metaldehyde poisoning of companion animals: a three-year retrospective study Full text
2017
Roma, Antonella De | Miletti, Gianluca | D’Alessio, Nicola | Rossini, Carmela | Vangone, Lucia | Galiero, Giorgio | Esposito, Mauro
Metaldehyde poisoning of companion animals: a three-year retrospective study Full text
2017
Roma, Antonella De | Miletti, Gianluca | D’Alessio, Nicola | Rossini, Carmela | Vangone, Lucia | Galiero, Giorgio | Esposito, Mauro
Introduction: Intentional and accidental poisoning of animals is often caused by readily available commercial pesticides, such as the molluscicide metaldehyde. A retrospective analysis of suspected metaldehyde poisonings between 2014 and 2016 in Italy was conducted. Material and Methods: Biological matrices were collected for toxicological analyses in the course of routine Institute activity. A total of 183 organs from dogs and cats and 49 pieces of bait, here specificall y poisoned food used to lure animals, were analysed and the presence of metaldehyde was confirmed by gas chromatography coupled to mass spectrometry (GC/MS). Results: A high content of metaldehyde was demonstrated in the analysed samples from dogs and cats: 50 (27.3%) animals were found positive for metaldehyde intoxication together with 17 pieces of bait. Conclusion: The study emphasised the need for the control of metaldehyde use by the veterinary service.
Show more [+] Less [-]Metaldehyde poisoning of companion animals: a three-year retrospective study Full text
2017
Roma Antonella De | Miletti Gianluca | D’Alessio Nicola | Rossini Carmela | Vangone Lucia | Galiero Giorgio | Esposito Mauro
Introduction: Intentional and accidental poisoning of animals is often caused by readily available commercial pesticides, such as the molluscicide metaldehyde. A retrospective analysis of suspected metaldehyde poisonings between 2014 and 2016 in Italy was conducted. Material and Methods: Biological matrices were collected for toxicological analyses in the course of routine Institute activity. A total of 183 organs from dogs and cats and 49 pieces of bait, here specificall y poisoned food used to lure animals, were analysed and the presence of metaldehyde was confirmed by gas chromatography coupled to mass spectrometry (GC/MS). Results: A high content of metaldehyde was demonstrated in the analysed samples from dogs and cats: 50 (27.3%) animals were found positive for metaldehyde intoxication together with 17 pieces of bait. Conclusion: The study emphasised the need for the control of metaldehyde use by the veterinary service.
Show more [+] Less [-]Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kałuża, Ewelina | Cepiel, Alicja | Pasławska, Urszula
Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kałuża, Ewelina | Cepiel, Alicja | Pasławska, Urszula
Introduction: The prevalence of arrhythmias in dogs and the influence of sex, breed, age, and body weight were analysed over a seven-year span. Material and Methods: In total, 1189 referrals for cardiological examination by electrocardiography were received at one academic centre in Poland between 2008 and 2014. The largest proportion of the examined dogs were cross-breeds with body weight below 25 kg (n = 153, 12.87%), followed by German Shepherds (n = 122, 10.26%), Labrador Retrievers (n = 68, 5.72%), Yorkshire Terriers (n = 63, 5.3%), and Boxers (n = 60, 5.05%). Retrospective analysis was made of 1201 standing or right recumbent electrocardiograms without pharmacological sedation. The prevalence of arrhythmias was examined in terms of sex, age, body weight, and breed of the dogs. Results: A total of 630 (52.46%) electrocardiograms showed no signs of arrhythmia, but 96 (7.99%) and 475 (39.55%) pointed to physiological and pathological arrhythmias respectively. The most commonly diagnosed type was atrial fibrillation with 33.68% incidence, followed by ventricular arrhythmias (28%), sinus pauses (27.58%), supraventricular arrhythmias (24%), and atrioventricular blocks (22.95%). Pathological arrhythmias were most commonly found in male dogs and in German Shepherds. Conclusions: Atrial fibrillation predominated, followed by premature ventricular complexes. Male dogs were generally more prone to heart rhythm disturbances.
Show more [+] Less [-]Prevalence of arrhythmias in dogs examined between 2008 and 2014 Full text
2017
Noszczyk-Nowak Agnieszka | Michałek Marcin | Kałuża Ewelina | Cepiel Alicja | Pasławska Urszula
Introduction: The prevalence of arrhythmias in dogs and the influence of sex, breed, age, and body weight were analysed over a seven-year span.
Show more [+] Less [-]Retrospective analysis of indications and complications related to implantation of permanent pacemaker: 25 years of experience in 31 dogs Full text
2019
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kapturska, Karolina | Cepiel, Alicja | Janiszewski, Adrian | Pasławski, Robert | Skrzypczak, Piotr | Pasławska, Urszula
Retrospective analysis of indications and complications related to implantation of permanent pacemaker: 25 years of experience in 31 dogs Full text
2019
Noszczyk-Nowak, Agnieszka | Michałek, Marcin | Kapturska, Karolina | Cepiel, Alicja | Janiszewski, Adrian | Pasławski, Robert | Skrzypczak, Piotr | Pasławska, Urszula
Introduction: Pacemaker implantation is the only effective symptomatic treatment for life-threatening bradyarrhythmias. Major complications observed after implantation of cardiac pacemakers include lead dislocation, loss of pulse generator function, and inadequate stimulation. The aim of this retrospective single-centre study was to analyse the indications for pacemaker implantation and the incidence and types of complications associated with this procedure in dogs treated for symptomatic bradyarrhythmia. Material and Methods: The retrospective analysis included 31 dogs with symptomatic bradyarrhythmia, implanted with permanent cardiac pacemakers in 1992–2017. The list of analysed variables included patient age, breed, sex, indication for pacemaker implantation, comorbidities, and the incidence of procedure-related complications along with the type thereof. Results: The most common indication for pacemaker implantation was 3ʳᵈ degree AVB, followed by SSS, advanced 2ⁿᵈ degree AVB, and PAS. Pacemaker implantation was associated with a 35% overall complication rate and 6.45% periprocedural mortality. There were no significant differences in terms of procedure-related complications with regard to age, sex, breed, indications for pacemaker implantation, or comorbidities. Conclusions: Cardiac pacing is the only effective treatment of symptomatic bradycardia, but as an invasive procedure, may pose a risk of various complications, including death.
Show more [+] Less [-]Retrospective analysis of indications and complications related to implantation of permanent pacemaker: 25 years of experience in 31 dogs Full text
2019
Noszczyk-Nowak Agnieszka | Michałek Marcin | Kapturska Karolina | Cepiel Alicja | Janiszewski Adrian | Pasławski Robert | Skrzypczak Piotr | Pasławska Urszula
Introduction: Pacemaker implantation is the only effective symptomatic treatment for life-threatening bradyarrhythmias. Major complications observed after implantation of cardiac pacemakers include lead dislocation, loss of pulse generator function, and inadequate stimulation. The aim of this retrospective single-centre study was to analyse the indications for pacemaker implantation and the incidence and types of complications associated with this procedure in dogs treated for symptomatic bradyarrhythmia.
Show more [+] Less [-]C-kit, flt-3, PDGFR-β, and VEGFR2 expression in canine adrenal tumors and correlation with outcome following adrenalectomy Full text
2021
Harding, Kayla | De Mello Souza, Carlos H. | Shiomitsu, Keijiro | Maxwell, Elizabeth | Bertran, Judit
The objective of this retrospective study was to evaluate the expression of receptor tyrosine kinases (RTKs) in canine adrenal tumors and correlate this expression with features of tumor aggressiveness and survival in dogs undergoing adrenalectomy. Forty-three canine adrenal tumors were evaluated for expression of c-kit, fms-like tyrosine kinase 3 (flt-3), platelet-derived growth factor receptor-β (PDGFR-β), and vascular endothelial growth factor receptor 2 (VEGFR2) using immunohistochemistry. Tumor RTK staining characteristics were compared to normal adrenals. Medical records were reviewed for data regarding patient outcome and tumor characteristics. Expression of c-kit, flt-3, PDGFR-β, and VEGFR2 was detected in 26.9%, 92.3%, 96.2%, and 61.5% of cortical tumors and 0%, 63.2%, 47.4%, and 15.8% of pheochromocytomas, respectively. Expression of RTKs was not significantly increased when compared to normal adrenals and did not correlate with survival after adrenalectomy. Receptor tyrosine kinases are not overexpressed in canine adrenal tumors compared to normal adrenal tissue. Therapeutic inhibition of these receptors may still represent an effective approach in cases where receptor activation is present.
Show more [+] Less [-]Retrospective quantitative assessment of liver size by measurement of radiographic liver area in small-breed dogs Full text
2019
OBJECTIVE To determine the feasibility of radiographic measurement of liver area in small-breed dogs and to assess correlations between CT liver volume measurements (reference standard) and radiographic liver size measurements. ANIMALS 107 small-breed dogs (body weight, ≤ 10 kg) that had previously undergone orthogonal thoracic and abdominal radiography and abdominal CT. PROCEDURES In a retrospective study design, dogs were allocated to groups (normal liver [n = 36], microhepatia [34], and hepatomegaly [37]) on the basis of radiographic liver size and clinicopathologic findings. Radiographic liver area (RLA) was automatically calculated from archived radiographic images by free-hand outlining of the liver margins by use of DICOM viewer software, and other standard radiographic measurements were performed. Liver volume was measured on CT images. Intraoperator repeatability of RLA and CT measurements was assessed (duplicate measurements 2 weeks apart). To control for various breed conformations, radiographic values were normalized to body weight and T11 area. RESULTS Mean ± SD ratios of RLA to T11 area and RLA to body weight for dogs with normal livers were 32.7 ± 6.2 and 7.0 ± 1.4, respectively. Excellent intraobserver agreement was observed in RLA measurements within groups (intraclass correlation coefficients, 0.861 to 0.989), and RLA measurements had the highest correlation with CT liver volume measurements (r = 0.94) of all radiographic measurements. CONCLUSIONS AND CLINICAL RELEVANCE Findings indicated that RLA measurement in small-breed dogs with or without liver disease was useful and accurate for estimation of liver size, compared with CT measurement, and might be particularly useful for monitoring of changes in liver size.
Show more [+] Less [-]Serum IgG response in calves to the putative pneumonic virulence factor Gs60 of Mannheimia haemolytica A1 Full text
2012
Orouji, Shahriar | Hodgins, Douglas C. | Lo, Reggie Y.C. | Shewen, Patricia E.
Bovine pneumonic pasteurellosis vaccines incorporate various antigens of Mannheimia haemolytica, including the acknowledged virulence factor leukotoxin (Lkt), and Gs60, a surface lipoprotein. To examine the role of antibodies to Gs60 in protection, an enzyme-linked immunosorbent assay (ELISA) was developed for retrospective analysis of serum samples from previous trials in which vaccines containing native or recombinant Gs60 were administered parenterally. The analysis revealed a positive correlation between the titer of antibodies to Gs60 and protection against experimental challenge in both vaccinates and naturally exposed controls. There was a strong correlation between production of IgG antibodies to Gs60 and Lkt neutralizing antibodies. Analysis of the relationship between the serum antibody titers and resistance to experimental challenge using linear statistical models revealed a significant association between prechallenge titers of serum antibodies to Lkt and protection. Further analysis suggested that antibodies against Gs60 were beneficial when Lkt neutralizing antibody titers were low.
Show more [+] Less [-]Accuracy of ultrasonography in the detection of severe hepatic lipidosis in cats
1992
Yeager, A.E. | Mohammed, H.
The accuracy of ultrasonography in detection of feline hepatic lipidosis was studied retrospectively. The following ultrasonographic criteria were associated positively with severe hepatic lipidosis: the liver hyperechoic, compared with falciform fat; the liver isoechoic or hyperechoic, compared with omental fat; poor visualization of intrahepatic vessel borders; and increased attenuation of sound by the liver. In a group of 36 cats with clinically apparent hepatobiliary disease and in which liver biopsy was done, liver hyperechoic, compared with falciform fat, was the best criterion for diagnosis of severe hepatic lipidosis with 91% sensitivity, 100% specificity, and 100% positive predictive value.
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