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Complete primary sequence of equine cartilage link protein deduced from complementary DNA.
1995
Dudhia J. | Platt D.
Investigation of the structure of equine articular cartilage link protein (LP) from individuals ranging in age from 1 to 15 years identified 3 distinct isoforms having molecular weights of 46,000, 43,000, and 41,000. The relative amounts of each of the 3 isoforms altered with age. The largest form did not change with age; however, amounts of the Mr 43,000 and 41,000 forms increased with increasing age. The results suggested that an accumulation, in the extracellular matrix of cartilage, of these 2 smaller products may have arisen from proteolytic cleavage. The complete amino acid sequence of the protein core was determined from complementary DNA products prepared by polymerase chain reaction amplification of cartilage LP mRNA. The sequence had 96% similarity with human LP and with that of other species for which the primary structure has been determined. This high degree of sequence conservation and the isoform data indicate that extracellular processing of LP occurs by similar mechanisms in various species. At the transcription level, equine chondrocytes were found to express LP as 2 abundant mRNA of 5.0 and 3.0 kb, and a smaller mRNA of 1.5 kb. Processing of the LP mRNA in horses, thus, appears to be similar to that found in other species investigated, and although multiple transcripts are present, the coding region remains unaltered and only 1 protein product is made.
Show more [+] Less [-]Nucleotide sequence of a gene encoding a new genus specific protein of Chlamydia psittacl
1991
Sato, C. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | katumata, A. | Takashima, I. | Hashimoto, N.
Characterization of DNA fragment from Chlamydia psittaci avian strain which shows high homology with HypB gene of Chlamydia
1991
Sato, C. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Katumata, A. | Takashima, I. | Hashimoto, N.
Studies on the cloning gp50 and gp63 genes of Pseudorabies virus (Shope strain)
1991
Kweon, C.H. | An, S.H. | Song, J.Y. | Kim, B.H. | Lee, J.B. | Lee, J.C. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Maeda, S. (Univ. of California, Davis (USA))
Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes
1995
Venter, E.H. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Veterinary and Tropical Diseases) | Huismans, H. | Van Dijk, A.A.
Cloning and sequencing of the gene encoding structural protein of canine parvovirus isolated in Korea
1992
Park, J.H. | Song, J.Y. | Lee, J.B. | Hyun, B.H. | An, S.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Inst.) | Jun, M.H. (Chungnam Nat'l Univ., Taejon (Korea Republic). Coll. of Veterinary Medicine)
