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Investigation of a listeriosis epizootic in sheep in New York state.
1997
Wiedmann M. | Arvik T. | Bruce J.L. | Neubauer J. | Piero F. del | Smith M.C. | Hurley J. | Mohammed H.O. | Batt C.A.
Acid-base and electrolyte balance in dairy heifers fed forage and concentrate rations: effects of sodium bicarbonate.
1987
Roby K.A.W. | Chalupa W. | Orsini J.A. | Elser A.H. | Kronfeld D.S.
Potential determinants of Clostridium spp. occurrence in Polish silage Full text
2020
Goldsztejn, Magdalena | Grenda, Tomasz | Kozieł, Nina | Sapała, Magdalena | Mazur, Małgorzata | Sieradzki, Zbigniew | Król, Beata | Kwiatek, Krzysztof
Potential determinants of Clostridium spp. occurrence in Polish silage Full text
2020
Goldsztejn, Magdalena | Grenda, Tomasz | Kozieł, Nina | Sapała, Magdalena | Mazur, Małgorzata | Sieradzki, Zbigniew | Król, Beata | Kwiatek, Krzysztof
Silage quality deteriorates with Clostridium spp. contamination, and if consumed, such silage jeopardises herd health and productivity. Minimising its occurrence reduces economic and animal welfare risks. The study investigated the influence of environmental and technological determinants on the Clostridium genus’ occurrence in silage. Analyses were conducted on 305 silage samples directly collected from farms located in all Polish provinces. Cultures and isolates were evaluated phenotypically and examined for occurrence of Clostridium spp., particularly C. perfringens and C. botulinum using PCR techniques. The results were statistically analysed using the ᵡ² test for continuous and Student’s t-test for non-continuous values. The most influential effect on Clostridium spp. occurrence is exerted by factors potentially associated with primary production, like the type of fertilisation and the contamination level of the ensiled feed material. Clostridium spp. was detected in 232 (76%) samples, and C. perfringens strains, predominantly toxinotype A, in 79 (26%). C. botulinum occurrence was not detected. Deterioration of silage by clostridia could be prevented by a properly conducted ensiling process with the addition of starter cultures, but the presence of spores mainly depends on primary production and the extent of contamination of the feed material.
Show more [+] Less [-]Potential determinants of Clostridium spp. occurrence in Polish silage Full text
2020
Goldsztejn Magdalena | Grenda Tomasz | Kozieł Nina | Sapała Magdalena | Mazur Małgorzata | Sieradzki Zbigniew | Król Beata | Kwiatek Krzysztof
Silage quality deteriorates with Clostridium spp. contamination, and if consumed, such silage jeopardises herd health and productivity. Minimising its occurrence reduces economic and animal welfare risks. The study investigated the influence of environmental and technological determinants on the Clostridium genus’ occurrence in silage.
Show more [+] Less [-]Animal botulism in Poland – laboratory and epidemiological investigations Full text
2022
Grenda, Tomasz | Goldsztejn, Magdalena | Kwiatek, Krzysztof | Kozak, Beata | Grenda, Anna
Animal botulism in Poland – laboratory and epidemiological investigations Full text
2022
Grenda, Tomasz | Goldsztejn, Magdalena | Kwiatek, Krzysztof | Kozak, Beata | Grenda, Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described. From 2019 to 2021, samples of serum, intestinal content, liver, spleen, kidney, faeces, wet feed, dry feed, ensilage, water and mixed samples of internal organs associated with 10 suspected animal botulism cases were sent to the National Veterinary Research Institute. Samples were analysed using a mouse bioassay and culture methods in combination with ntnh and bont gene detection. Among the ten putative botulism cases, only four (40%) were confirmed in the laboratory on the basis of the detection of botulinum toxin (BoNT) or the ntnh or bont genes. The remaining six (60%) were determined as probable despite observable characteristic clinical signs. The diagnosis of botulism in animals is a very difficult task, made so by the heterogeneity of Clostridium botulinum strains and possible loss of toxinogenicity during laboratory processing or the potential degradation of toxins. Laboratory diagnosis is a complex and problematic process which should utilise different prescribed methods for specific types of sample.
Show more [+] Less [-]Animal botulism in Poland – laboratory and epidemiological investigations Full text
2022
Grenda Tomasz | Goldsztejn Magdalena | Kwiatek Krzysztof | Kozak Beata | Grenda Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described.
Show more [+] Less [-]Epidemiologic investigation of a silage-associated epizootic of ovine listeric encephalitis, using a new Listeria-selective enumeration medium and phage typing
1992
Vazquez-Boland, J.A. | Dominguez, L. | Blanco, M. | Rocourt, J. | Fernandez-Garyzabal, J.F. | Gutierrez, C.B. | Tascon, R.I. | Rodriguez-Ferri, E.F.
The role of silage feeding in the origin of an epizootic of encephalitic listeriosis in a sheep flock was investigated by use of a new direct Listeria-selective isolation and enumeration medium, in combination with serotyping and phage typing. The silage contained high numbers (about 10(6) cells/g) of a L monocytogenes strain indistinguishable with respect to serovar and phagovar from that isolated from the brains of sick sheep. These results provided unambiguous bacteriologic evidence of the epidemiologic link between silage consumption and listeriosis in ruminants.
Show more [+] Less [-]Mixed Leucaena and molasses can increase the nutritional quality and rumen degradation of corn stover silage Full text
2023
Yusuf Akhyar Sutaryono | Ryan Aryadin Putra | Mardiansyah Mardiansyah | Enny Yuliani | Harjono Harjono | Mastur Mastur | Sukarne Sukarne | Luh Sri Enawati | Dahlanuddin Dahlanuddin
Objective: The study was conducted to determine the effect of Leucaena at different proportions and doses of molasses on the nutritional quality, silage fermentation characteristic, and in vitro digestibility of corn stover silage. Materials and Methods: The study was designed in a completely randomized factorial design 3*3 pattern. The first factor was the proportion addition of Leucaena, i.e., L0 (0%), L15 (15%), L30 (30%), and L45 (45%) of inclusion of Leucaena on the dry matter (DM) basis of corn stover. The second factor was the dose of inclusion of molasses, i.e., M2 (2%), M4 (4%), and M6 (6%) on the fed basis of silage. Each treatment had five replications. The variables observed included chemical composition [DM, organic matter (OM), crude protein (CP), crude fiber (CF), hemicellulose, acid detergent fiber, and neutral detergent fiber], silage fermentation characteristics (pH and NH3-N), DM digestibility (DMD), and OM digestibility (OMD) under in vitro conditions. Results: The result shows that the inclusion of Leucaena in the proportion of 30%–45% is very effective in increasing and improving the chemical composition of corn stover silage, significantly suppresses the content of CF, and increases the CP content of the silage. Likewise, the inclusion of molasses at a dose of 4% also positively contributed to the quality of the resulting silage, especially its effect in suppressing the buffer capacity of proteins resulting in low pH values and NH3-N concentrations in silage. Conclusions: It was concluded that the inclusion of Leucaena in 30%–45% and the inclusion of molasses at a dose of 4% is very effective in increasing and improving the chemical composition, silage fermentability characteristics, and rumen degradation of corn stover silage. [J Adv Vet Anim Res 2023; 10(1.000): 118-125]
Show more [+] Less [-]Влияние скармливания силоса, заготовленного с консервантами Кормоплюс, на переваримость рационов
2009
Tsaj, V.P., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Usage of preservatives Kormoplus 1 and 2 lets us produce the silage of a highest quality and raise dry substance integrity at 8,7%, protein - at 12-9,4%. The researches determined that cereal silage, prepared with supplement Kormoplus 1 and 2, lets us raise digestibility of dry substance at 5,5% and 3,7%, organic substance - at t 5,5 and 4,0%, fiber- at 16,5 and 15,0% and raise nitrogen sediment at 17,4 and 20,8%. These forages didn't have any negative influence at health and biochemical performance of cow blood
Show more [+] Less [-]Консервирование злаково-бобовой массы с биопрепаратами из сапропеля
2008
Dobruk, E.A. | Pestis, V.K. | Sarnatskaya, P.P. | Frolova, L.M., Grodno State Agrarian Univ. (Belarus) | Yakovchik, N.S., Breeding Farm Zakozelskij, Drogichin dist. (Belarus)
Results of studies of the influence of two biological products produced on the basis of sapropel (putrid mud) of the Ganarata lake (Mostovski district, the Republic of Belarus) on quality of legume-grass silage were presented. It was established, that analyzed preparations could be used as the preserving agents for preparation of herbal forages. The preserving action of the preparations was caused by the intensification of lactic bacteria development in silaging mass for the account of humic acids, as well as by the fungicidal action of phenolic and carboxylic fractions of the preparation. Silage preparation in combinations with putrid mud preparations made it possible to lower the losses of a crude protein on 11,3-15,6%, sugar - on 33,3%, carotin - on 20% and to obtain forage with concentration of exchange energy 9,20-9,38 mJ per 1 kg of dry matter. Silage application in combination with biological products rendered positive impact on milking efficiency of cows, the average daily milk yields in the experimental cow groups were on 1,3-1,4 kg or 6,8-7,3% higher. Application of higher quality feeds made it possible to lower the expenses of forages on 0,04 fodder units. Research results proved that the analysed preparations from sapropel could be used as an efficient conservants for silage preparations. They made it possible to raise forage quality at the minimum expenses
Show more [+] Less [-]Злаковый силос, заготовленный с консервантом Axphast Gold, в рационах крупного рогатого скота
2008
Tsaj, V.P. | Gurin, V.K., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
The comparative analysis of application of cereal ensilage produced with addition of microbial and enzymatic preserving agents Axphast Gold and Biotrof on fodder digestibility, hematological indexes and cicatrical digestion of cattle rearers was realized in the conditions of the Republic of Belarus. Cattle feeding with the analysed silage rendered positive effect on digestibility of the forage dry matter, which was on 2% higher the control index, organic matter - on 2,4%, nitrogen-free extractive substances - on 2,84%, fat - on 0,58%, protein - on 2,3%, cellulose - on 2,1%. Use in feeding of ensilaged forages from the cereal perennial grasses preserved by the microbial- enzymatic preparation produced by company Biota rendered the positive influence on use of nitrogen, calcium and phosphorus. There was also noted the increasing of the content of volatile fatty acids in paunch of young cattle silage fed with Axphast Gold that testified of more effective utilization of forage
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