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Rapid differentiation of Hanwoo and Holstein meat using multiplex allele specific polymerase chain reaction protocols
2005
Koh, B.R.D. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea), E-mail: barada@nvrqs.go.kr
Here I describe a multiplex allele specific PCR-based approach for the rapid detection between Hanwoo and Holstein meat associated with Melanocortin 1 receptor (MC1R) gene. Specific and universal oligonucleotide primers were used in combination to detect the presence of a single nucleotide polymorphism within the bovine MC1R DNA sequence. The presence of the bovine MC1R gene is indicated by the production of a single control PCR product, whilst positive samples generate an alternative smaller specific product over the same region. The mutations in MC1R104 codon revealed depending on the presence or absence of an indicative fragment amplified from the wild-type allele of this codon.
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