Introduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.

Body and testis weights, serum luteinizing hormone, follicle-stimulating hormone, and prolactin values and volume fractions of Sertoli cells, spermatogonia, early and late primary spermatocytes, and round and long spermatids were evaluated in 70-day-old male rats treated orally with 20 mg of zearalenone/kg of body weight daily for 5 weeks. A significant (P < 0.05) increase in serum prolactin concentration was consistently observed during the 5 weeks of treatment with zearalenone. Significant changes were not observed in any of the other variables evaluated.

The present study was undertaken to induce gonadal maturation of Mrigal, Cirrhinus mrigala using Human Chorionic Gonadotropin (HCG) implants in captive conditions. The male and female fishes of C. mrigala were implanted intramuscularly with hCG implants at the dosage of 1000 IU/kg body weight, to study the effect of hCG hormone. Hormone implants were given once in two months during the experimental period of February to June. Histological observation in the ovary of C. mrigala revealed the presence of four types of growing oocytes namely perinucleolar, previtellogenic, vitellogenic and hydrated oocytes. Histological observation of the testis of C. mrigala showed the presence of five types of spermatogenic cell types namely spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. Hence, the present study recommends that hCG implants can successfully be used to induce sustained maturation of C. mrigala in captivity even during off season.&nbsp;