Comparative evaluation of the influence of cell-free probiotic preparation Laktimet and cellular probiotic Bifilak on productivity, livability, nonspecific response of suckling piglets in the early extrauterine life was realized in the conditions of the Republic of Belarus.Application of both probiotics increased the productivity of suckling pigs, stimulated the processes of metabolism and natural protection of organism in the early postnatal period in the conditions of specialized pig breeding complexes. Research results showed, that application of brand new cell-free probiotic Laktimet exercised higher influence on suckling pigs. Application of Laktimet made it possibler to increase piglets body weight gain on 7,8%; piglet livability - on 6,7%; and application cellular probiotic preparation Bifilak - on 5,4% and 3,3%, respectively. The same tendency was noted during the analysis of indexes of cellular factors of organism protection. Bactericidal activity of blood serum of experimental animals changed both in connection with age, and under influence of probiotic preparations. During all studied periods the bactericidal activity of blood serum was authentically (P less than 0,05) above in experimental groups in comparison with a control group. At 20-days old piglets there was stated the strengthening of bactericidal activity of blood serum. Throughout all period of experiment the superiority of trial animals over control on indicators of lysozyme activity of blood serum was observed

The data on an inactivation scheme for the agent of porcine pasteurellosis, development of the inactivated vaccine and ratio of the antigen and adjuvant are presented. As a result of the realized research it has been established, that for Pasteurella inactivation it is necessary to use formalin in concentration of 0,5% at an exposition at 1 hour. At vaccine producing it is necessary to apply an antigene parity to adjuvant 50:50 as the received vaccine does not concede on all parameters to the vaccine in which a parity of components accordingly 40:60. Thus received vaccine possesses smaller reactogenicity and more adjuvanticity. One of the basic conditions of reception of homogeneous stable emulsion is warming up of water and oil phases to temperature 30 deg С (+/-1 deg С). | Представлены сведения о разработке схемы инактивации возбудителя для изготовления инактивированной вакцины, а также о выборе соотношения антигена и адъюванта. В результате проведенных исследований было установлено, что для инактивации пастерелл следует использовать формалин в концентрации 0,5% при экспозиции в 1 час. При изготовлении вакцины необходимо применять соотношение антигена к адъюванту 50:50, так как полученная вакцина не уступает по всем параметрам вакцине, в которой соотношение компонентов соответственно 40:60. При этом полученная вакцина обладает меньшей реактогенностью и большей иммуногенностью. Одним из основных условий получения однородной стабильной эмульсии является подогревание водной и масляной фаз до температуры 30 deg С (+/-1 deg С).

There was studied the width of distribution and peculiarity of clinical manifestation of mineral exchange diseases at pregnant sows, and also perfection of preventive actions at pathology of mineral metabolism in the conditions of prophylactic medical examination. Results of research have shown, that diseases of mineral metabolism widespread (84,7%) among pregnant sows in the conditions of industrial production, are most extended scarce conditions on Zn (17,7%) and Fe (22,5%). Clinically this insufficiency was expressed by oppression, appetite distortion, occurrence on a skin of specifically red stains correct roundish the form: with the clarified sites in the centre, are registered as abortions at final stages of pregnancy. It is established, that the way of preventive maintenance of hypomicroelementosis of pregnant sows with use of chelated forms of microelements is more preferable than sows, as reduces percent of clinical display of Zn and Fe deficiency, stabilize level of hematological and biochemical indicators of blood more effectively, and also raise industrial indicators of pregnant sows. | Целью работы было изучение широты распространения и особенностей клинического проявления заболеваний минерального обмена у супоросных свиноматок, а также совершенствование профилактических мероприятий при патологии минерального обмена веществ в условиях диспансеризации. Результаты исследований показали, что болезни минерального обмена веществ широко распространены (84,7%) среди супоросных свино¬маток в условиях промышленного производства, наиболее распространены дефицитные состояния по Zn (17,7%) и Fe (22,5%). Клинически эта сочетанная недостаточность выражалась угнетением, залеживанием, понижением и извращением аппетита, появлением на коже специфически красных пятен правильной округлой форма: с просветленными участками в центре, зарегистрированы так же аборты на конечных стадиях супоросности. Установлено, что способ профилактики гипомикроэлементозов супоросных свиноматок с использованием хелатных форм микроэлементов предпочтительнее, поскольку сокращает процент клинического проявления дефицита цинка и железа, эффективнее стабилизируют уровень гематологических и биохимических показателей крови, а также повышают производственные показатели супоросных свиноматок.

As a result of realized in the conditions of the Republic of Belarus research it was revealed that application of immunostimulative drugs of sodium thyosulphate and vitamin С (ascorbic acid) in combination with a vaccine against salmonellosis, pasteurellosis and streptococcosis of pigs promoted the statistically authentic increasing of leukocytes, absolute quantity of T- and B-lymphocytes content in peripheral blood. Also, it promoted the activation of phagocytic activity and digested ability of neutrophils; activization of mieloblastic hemopoiesis and decreasing of erythrocytopoiesis; activization of morphological reactions in a tissue at a place of administration of the vaccine, lymphatic nodules and spleen. | Проведенные исследования показали, что применение иммуностимуляторов натрия тиосульфата и витамина С с вакциной против сальмонеллеза, пастереллеза и стрептококкоза свиней способствует статистически достоверному повышению в периферической крови содержания лейкоцитов, абсолютного количества Т- и В-лимфоцитов, активизации фагоцитарной активности и перевариваемой способности нейтрофилов; активизации миелобластического кроветворения и снижению эритропоэза; активизации морфологических реакций в ткани на месте введения вакцины, лимфоузлах и селезенке.

Belarus concerns a biogeochemical province with the lowered content in environment of such microelements, as iodine, selenium, copper, cobalt, manganese, zinc and some other. It conducts to infringement of intensity and an orientation of processes of an exchange of proteins, carbohydrates, lipids, and finally - to decrease in their growth and development of an organism of young growth. High cost of foreign preparations for treatment and preventive maintenance of an alimentary anaemia, low bioavailability of iron and toxicity do not provide purposeful disesase control and immunodeficiencies arising on its background. The special urgency is got by research of means of complex preventive maintenance of an alimentary anaemia and immunodeficiencies. It has been established that the recipes N1-3 have proved to be non-toxic for laboratory animals and have no effect on the meat quality.

In the conditions of the Republic of Belarus there was determined the parasitizing influence of Oesophagostomum and their larvae on a metabolism of experimentally infested animals. In course of the study there were generated experimental and control groups of two-month old piglets. Animals of the experimental group were infested in a dose of 15000 invasion larvae per one kg of body weight. Animals of the control group were not infested. Infestation was realized through a mouth with invasion larvae with wet forage. Invasion larvae received by cultivation of excrement tests from infested pigs at temperature of 24 deg C. As a result of the realized research it was established, that Oesophagostomum invasion rendered a substantial influence on the experimental animals. At bimestrious pigs after the experimental infection with Oesophagostomum larvae there were stated the following changes: the disease on 4-30th day was characterized by frustration of function of a gastroenteric path - diarrhea, fever, anaemia, and in the subsequent - stagnation and juvenilism of animals. Oesophagostomum in a host organism caused the pathological changes of haemotological and biochemical blood value which were expressed in quantity decrease of erythrocytes, concentration of haemoglobin, leucocytosis. Eosinophilia was observed in a leukogram. In blood serum there was stated the lowering of crude protein and its fractions content; gradually, but authentically there was noted the increasing of activity serum glutamic oxalacetic transaminase and glutamic-pyruvic transaminase. Bactericidal and lysozymic activity of blood serum decreased. Activity of alkaline phosphatase increased.

In the conditions of the Republic of Belarus there was studied the influence of herbal infusion, tincture, liquid and dry extracts of elecampane (Inula helenium) on morphological and biochemical indexes of pig blood. In course of the study it was established, that preparative forms of elecampane stimulated the formation of blood corpuscle, and also increased of the hemoglobin level in blood of pigs. Administration of elecampane preparations did not cause any authentic changes in blood leukogram of swine; all indicators were within normal limits. Elecampane preparations did not cause changes on the part of biochemical blood indexes of and did not influence the activity of enzymes. Research results showed that herbal infusion, tincture, liquid and dry extracts of elecampane did not render negative influence on swine.

In the conditions of the Republic of Belarus there were analyzed productivity indexes of sows of different families and family groups and their compatibility with male pigs of different lines of Belarusian meaty breed and Landras. On the basis of the realized research it was marked, that according to the number of piglets in a litter and multiple pregnancy distinctions between breeding sows of Landras breed and Belarusian meaty there was not observed. From sows of both breeds in average per farrowing there was obtain 11,0 - 11,2 piglets, including 10,4 - 10,5 live piglets, that exceeded requirements of the first class of the instruction on evaluation of pigs on 4,0 - 5,0%. At the same time, sows of the Belarusian meaty breed had litter weight at weaning in 40 days of 104 kg, that was authentically higher on 8 kg or on 8,3% (Р less than 0,001), than sows of Landras breed. In each breed there were some distinctions in sow productivity by families and in breeding with boars of different lines.

In the conditions of the Republic of Belarus there was studied the propagation of associative parasitic diseases of swine of different age-specific groups, as well as their influence on composition of microbial flora of large intestinal tract. As a result of realized studies in different age-sex groups of pigs there were revealed some monoinfections (A. suum; T. suis; Eimeria sp.) and associations (A. sum; T. suis; Eimeria sp.; Oesophagostomum dendatum; B. coli) in different ratio (depending on sex and age-specific groups). There were revealed a certain peculiar features in changing of microbiocenosis of large intestinal tract of swine under the influence of parasitic agents (namely, in cases of parasitic diseases caused by A. suum; T. suis; Eimeria sp.; O. dendatum; B. coli both in the form of mono infestations and in associations). These features were expressed by lowering of standard flora quantity (bifid bacterium and lactic acid bacillus) with a simultaneous increasing of the content of opportunistic coliform bacillus, aerobic bacteria, bacilli, and micromycetes.

In the conditions of the Republic of Belarus there were analyzed the doses and efficiency of application of selenium in composition of a mixed fodder KP-2 in diets of bull calves of at their breeding for meat. In course of the study the was developed a formula of a mixed fodder with various doses (0,1; 0,2; 0,3 mg per 1 kg of a dry matter of a ration) of selenium; there was studied the influence of different selenium doses in mixed fodders on palatability of diets, their digestibility, use of nutritive elements and hematological indexes of animals. Also, there was analyzed the influence of different doses of selenium on bull calves growing intensity. There was given the zootechnical and economic evaluation of usage of selenium for bull calves. Selenium dose of 0.2 milligram per 1 kilogram of dry matter proved to be the most efficient and promoted the increasing of average daily body weight gain on 10.9%.