Refine search
Results 1-7 of 7
Application of immunohistochemical technique for diagnosis of porcine epidemic diarrhea
1994
Park, N.Y. | Cho, K.O. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Histopathological and immunohistochemical studies on the intestinal lesions related to the infected age in spontaneous canine parvovirus enteritis
1994
Ku, J.L. | Seo, I.B. | Lim, C.H. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
A study on production of early pregnancy diagnostic kit in cattle, 1; Production of polyclonal antibody to progesterone and removal of anti-bovine serum albumin antisera
1991
Kang, C.B. | Lee, H.J. | Choe, S.Y. (Gyeongsang National Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application., 2; establishment of enzyme immunoassay for progesterone
1989
Kang, C.B. | Shin, J.U. | Choe, S.Y. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine)
This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, enzyme conjugate and gelatin were investigated. The sensitivity, recovery rate and reproducibility by this assay were also analyzed. The suitable supplementation level of gelatin was 0.2 %. As the gelatin level increased to 1 %, coefficient variation of interassay was shown to be irregular. The optimum dilution rate of enzyme conjugate was 30 times. Enzyme activity was greatly fluctuated depending on the minor condition of enzyme conjugate technique. Therefore, it was considered to be checked when determined. The sensitivity of the assay was 12 pg/tube. Recovery rate was decreased when the amount of sample was too little or too much, but the recovery rate was high as 97.8 % when the amount of sample was between 50 and 200 micro l. Mean intra-assay and inter-assay coefficient of variation was 4.5 % and 5.9 %, respectively. By using liquid phase double antibody enzyme immunoassay, progesterone in plasma can be detected, and also this assay system is applicable to study the physiological function of progesterone and diagnosis of reproductive disorders
Show more [+] Less [-]Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application: 1. Optimizing double antibody for progesterone in enzyme immunoassay
1988
Kang, C.B. | Shin, J.U. | Choe, S.Y. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Agruculture)
This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was 10X10** (3) to 15X10** (3), 20 and 1X10** (3) times, respectively
Show more [+] Less [-]Distribution of antimicrobial resistances and properties of R-plasmids in E coli isolated from pigs
1994
Chung, M.E. | Yeo, S.G. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
Optimization of liquid phase enzyme immunoassay for determining of progesterone
1992
Kang, C.B. | Choi, I.K. | Son, M.S. | Hur, J.H. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine) | Kim, C.H. (Gyeongsangnam Do Veterinary Service Laboratory, Chinju (Korea Republic))
