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Comparison of latex agglutination, indirect immunofluorescent antibody, and enzyme immunoassay methods for serodiagnosis of Rocky Mountain spotted fever in dogs.
1993
Greene C.E. | Marks M.A. | Lappin M.R. | Breitschwerdt E.B. | Wolski N.A. | Burgdorfer W.
Indirect immunofluorescent antibody (IFA), latex agglutination (IA), and enzyme immunoassay (EIA) methods were compared for evaluation of the serum antibody responses of dogs experimentally and naturally exposed to spotted fever-group rickettsiae. Selected sera (obtained on days 1, 42, 53, 124, 145, 236, 255, 264, and 292) were examined from three 8-month-old female Beagles inoculated with Rickettsia rickettsii on days 34 and 250 of the study. A second group of dogs comprised three 8-month-old female Beagles inoculated with R montana on days 34 and 102. Subsequently, these dogs were inoculated with R rickettsii on day 250. Serum samples were obtained from the second group of dogs on days 1, 96, 103, 132, 180, 215, 292, and 494. A third group consisted of 21 naturally exposed dogs, from which sequentially obtained serum samples were available, and which had clinical signs compatible with Rocky Mountain spotted fever. Clinical signs of disease in dogs of the third group resolved after treatment with tetracycline (22 mg/kg of body weight, Po, q 8 h) was instituted. At least 2 sequentially obtained serum samples from each dog were tested. In general, the first sample was obtained just prior to treatment and the convalescent serum samples were obtained at weekly or greater intervals thereafter. For correlation and reactivity data, an IFA test for IgG/IgM (using heavy and light chains-specific conjugate) was used as the reference standard for comparison of results with those of the other tests,.
Show more [+] Less [-]PMSG profiles in superovulated and anti-PMSG antiserum treated mice and heifers with enzymeimmunossay.
1991
Katagiri S. | Takahashi Y. | Hishinuma M. | Kanagawa H. | Dochi O. | Takakura H.
Development of an enzyme immunoassay for determination of steroid hormones to improve the reproductive efficiency of domestic animals.
1993
Choi H.S. | Kang B.K.
Immunohistochemical identification of Newcastle disease virus with indirect immunoperoxidase technique.
1990
Nho W.G. | Sur J.H. | Kim S.B.
Distribution of antimicrobial resistances and properties of R-plasmids in E coli isolated from pigs
1994
Chung, M.E. | Yeo, S.G. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
Optimization of liquid phase enzyme immunoassay for determining of progesterone
1992
Kang, C.B. | Choi, I.K. | Son, M.S. | Hur, J.H. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine) | Kim, C.H. (Gyeongsangnam Do Veterinary Service Laboratory, Chinju (Korea Republic))
Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)
1997
Habibur Rahman, A. (Institute of Veterinary Preventive Medicine, Ranipet (India)) | Manickam, R.
Application of immunohistochemical technique for diagnosis of porcine epidemic diarrhea
1994
Park, N.Y. | Cho, K.O. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Histopathological and immunohistochemical studies on the intestinal lesions related to the infected age in spontaneous canine parvovirus enteritis
1994
Ku, J.L. | Seo, I.B. | Lim, C.H. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
Application of immunoperoxidase techniques to formalin-fixed brain tissue for the diagnosis of rabies in southern Africa
1994
Last, R.D. (Allerton Regional Laboratory, Pietermaritzburg (South Africa)) | Smit, M.M.E. | Van der Lugt, J.J.
