Supernumerary ectopic limb(s) (SEL) is a congenital anomaly defined as the presence of accessory limb(s) attached to various body regions. This paper describes a case of SEL with ectopic lung and ectopia cordis in a newborn calf, based on macroscopic, microscopic and radiographic findings. External features of multiple congenital anomalies included an ectopic lung growing over the middle of the backbone and covered with normal haired skin. Ectopia cordis was found in the abdominal cavity and attached to the liver. Two extra abnormal limbs originated separately from within the ectopic lung. Most of the abdominal organs were exposed to the outside through the opened abdominal cavity. Microscopically the ectopic lung tissue had edema in the connective tissue around the bronchus and artery. Changes in other organs included congestion of the renal medulla, infiltration of inflammatory cells (lymphocytes and eosinophils) around the hepatic portal tract, and edema surrounding blood vessels and neurons in the brain. The rudimentary humerus of the forelimb was attached to the thoracic spine, as viewed radiographically. The hindlimb was consisted of an irregularly shaped femur, short tibia and fibula, two tarsal bones, one metatarsal bone, and three phalanges. This is the first description of congenital anomalies involving the SEL, ectopic lung and ectopia cordis in a calf.

Outer membrane proteins (OMPs) of Pasteurella multocida strain P52 were purified by immunoaffinity chromatography (IAC) using pre-challenge serum from HS vaccinated cattle-calves. IgG antibody titers of pre-challenge sera from HS vaccinated calves surviving and succumbing to direct challenge with virulent culture were determined against formalized P52 cells, P52 crude cell lysate, and OMPs of P52 purified by IAC. It was observed that OMP antigens were involved with protective response in calves and indirect ELISA test using lAC purified antigens can be developed for in vitro potency determination of Haemorrhagic septicemia (HS) vaccines.