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THE IMPACT THERMAL STRESS ON SOME PHYSIOLOGICAL, ENDOCRINE PROFILES AND HSP IN LOCAL MALE CALVES Full text
2020
Jinan A. Hilal | Muna H. AL-Saeed
This study was conducted in the field cattle in Al-Qurna, north of BasraGovernorate, in the period between July 2018 to February 2019 and included thefollowing seasons, the monthly summer season(July and August of 2018), themonthly autumn season (October and November of 2018) and the winter season ismonthly (January and February of 2019) .This experiment was designed to reveal theeffect of the THI value on some physiological and hormonal values on the local maleHolstein. The studied group include 20 calves and with age between (one year to oneyear and eight months), twenty blood samples were collected per month from maleHolstein calves subjected to a different value of temperature and humidity in differentmonths and seasons.The results of the first experiment showed: A significant(P≤0.05)increase in the values of THI, respiratory rate, and heart rate, where asignificant(P≤0.05) increase was noted in months and the summer season except forthe rectal temperature, which showed no significant difference (P˃0.05) amongdifferent seasons. The antioxidants CAT, MDA, SOD, and GPX also showed asignificant increase (P≤0.05) during the summer months and seasons compared to theautumn and winter months and seasons. Also significant increase (P≤0.05) in cortisoland HSP70 during the summer and season months.The levels of testosterone andthyroid hormone (T4) and triiodothyronine (T3) decreased significantly (P≤0.05)during summer and season compared to other months and seasons, but the level ofinsulin in the blood showed a significant decrease (P≤0.05) during the summer seasonthan the other seasons.
Show more [+] Less [-]HISTOLOGICAL AND MOLECULAR STUDY OF SPLEEN IN Japanese Quail UNDER THERMAL CONDITION Full text
2017
Zainab A. Al-Ali Majdy Faisal Jalal yaseen
The present study was conducted to determine the histological and Molecular changes in the spleen of adult male Japanese quail after being exposed to effect of thermal stress was recorded. This study included two groups, each group consisting twelve male birds, the control group (A) was exposed to normal temperature for 45days, while the group (B) exposed to temperature (42OC) for 45 days. The histological and molecular changes were studied during (15, 30 and 45) days of the experiment, histological changes in spleen were represented by necrosis, degeneration hemorrhage and shrinkage fibrosis of lymphocytic nodules, cytoplasmic vaculation, infiltration of lymphocytes, metamorphosis of lymphocyte, nuclei pyknotic, dilation in the central vein of lymphatic nodules, edema, dilation of sinusoids and congested blood vessels. Molecular examination showed that the heat shock protein (hsp70) gen is present in temperature group that is also found in the control group. This confirms that the hsp70 gen is present in birds at normal and abnormal conditions.
Show more [+] Less [-]Evaluating fertility and growth rate potential of indigenous sheep breeds submitted to heat stress under different management systems Full text
2020
Shakirullah Khan | Imdad Ullah Khan | Alam Zeb Khan | Shahid Zaman | Abdul Majid | Atiq Ur Rehman | Mumtaz Ali Khan | Hamza Maris | Rahman Ullah | Subhan Qureshi
Objective: Sheep farming is an important source of livelihood in the southern Khyber Pakhtun Khwa province of Pakistan where they are reared under extensive system (ES). This system has limitations of improper feeding, rearing, and management which negatively affect their productivity. The pres¬ent study compares different rearing systems and their stresses on fertility, behavior, and growth rate potential of two sheep breeds of Pakistan. Materials and Methods: Eighty ewes were selected; forty each from Damani and Balkhi breeds reared in semi-intensive system (SIS) and ES at Paharpur district, Dera Ismail Khan. Blood samples were collected in triplicate on days 0, 14, and 28 of May, and the molecular and behavioral stress, growth, and fertility rates were recorded. Results: The highest growth and fertility rate were observed in both the breeds in SIS than in ES (p < 0.01). The values of stress indicators (cortisol, heat shock protein (HSP-70)) and behavioral stress parameters were found lower in Damani breed as compared to Balkhi breed in both rearing systems (p < 0.01). All the molecular stress and behavioral stress parameters were negatively associated with metabolic hormones (T3 and T4) (p < 0.01). A positive correlation was observed among all the molecular stress and behavioral stress parameters (p < 0.01). Similarly, T3 and T4 were positively interlinked to one another (p < 0.01). Conclusion: It was concluded that both Damani and Balkhi sheep showed lower stress levels and better fertility and growth parameter in SIS than ES system of rearing. Local Damani breed exhibited well tolerant ability and genetic adaptation to the local environment as compared to Balkhi breed. [J Adv Vet Anim Res 2020; 7(1.000): 170-176]
Show more [+] Less [-]Influence of temperature on Streptococcus agalactiae infection in Nile tilapia Full text
2015
Paulo Fernandes Marcusso | Jefferson Yunis Aguinaga | Gustavo da Silva Claudiano | Silas Fernandes Eto | Dayanne Carla Fernandes | Hurzana Mello | Fausto de Almeida Marinho Neto | Rogério Salvador | Julieta Rodini Engrárcia de Moraes | Flávio Ruas de Moraes
Environmental changes affect fish homeostasis, turning them more susceptible to diseases. In Brazil, outbreaks of Streptococcus agalactiae infection have been reported in Nile tilapia when they are outside of their thermal comfort zone. This investigation evaluated mortality rate and which were the most infected organs at temperatures that naturally occurred in southern of Brazil. Forty Nile tilapia (Oreochromis niloticus) were infected with S. agalactiae and distributed in four groups (n=10) and each group was exposed to a different temperature: G1: 24°C, G2: 26°C, G3: 28°C, and G4: 32°C. Fish were monitored for 10 days. In this period, fish that presented irreversible clinical signs were sacrificed and samples of brain, liver, and kidney were collected for bacteriological and molecular analysis. Signs compatible with a streptococcal infection were observed in all groups. Highest mortality rates occurred at 24°C and 32°C. The brain was the most affected organ with the highest percentage of isolation of S. agalactiae by both methods of diagnosis. The results suggest that, as in mammals, temperatures that are further away from the comfort zone influence fish homeostasis, increasing susceptibility to bacterial infections.
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