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16S rRNA gene profiling of bacterial communities mediating production of tsetse attractive phenols in mammalian urine
2019
Musonye, Harry A.(Kenyatta University Department of Biochemistry, Microbiology and Biotechnology) | Njeru, Ezekiel M.(Kenyatta University Department of Biochemistry, Microbiology and Biotechnology) | Hassanali, Ahmed(Kenyatta University Department of Chemistry) | Langata, Lydia M.(Kenyatta University Department of Biochemistry, Microbiology and Biotechnology) | Mijele, Dominic(Kenya Wildlife Service Department of Veterinary Services) | Kaitho, Titus(Kenya Wildlife Service Department of Veterinary Services) | King'ori, Edward(Kenya Wildlife Service Department of Veterinary Services) | Nonoh, James(Kenyatta University Department of Biochemistry, Microbiology and Biotechnology)
Several types of odours are involved in the location of host animals by tsetse (Diptera: Glossinidae), a vector of animal African trypanosomiasis. Host animals' ageing urine has been shown to be the source of a phenolic blend attractive to the tsetse. Nevertheless, limited research has been performed on the microbial communities' role in the production of phenols. This study aimed at profiling bacterial communities mediating the production of tsetse attractive phenols in mammalian urine. Urine samples were collected from African buffalo (Syncerus caffer), cattle (Bos taurus) and eland (Taurotragus oryx) at Kongoni Game Valley Ranch and Kenyatta University in Kenya. Urine samples, of each animal species, were pooled and left open to age in ambient conditions. Bacteriological and phenols analyses were then carried out, at 4 days ageing intervals, for 24 days. Phenols analysis revealed nine volatile phenols: 4-cresol, ortho-cresol, 3-cresol, phenol, 3-ethylphenol, 3-propylphenol, 2-methyloxyphenol, 4-ethylphenol and 4-propylphenol. Eight out of 19 bacterial isolates from the ageing urine revealed the potential to mediate production of phenols. 16S rRNA gene characterisation of the isolates closely resembled Enterococcus faecalis KUB3006, Psychrobacter alimentarius PAMC 27887, Streptococcus agalactiae 2603V, Morganella morganii sub.sp. morganii KT, Micrococcus luteus NCTC2665, Planococcus massiliensis strain ES2, Ochrobactrum pituitosum AA2 and Enterococcus faecalis OGIRF. This study established that some of the phenols emitted from mammalian urine, which influence the tsetse's host-seeking behaviour, are well characterised by certain bacteria. These results may allow the development of biotechnological models in vector control that combines the use of these bacteria in the controlled release of semiochemicals.
Show more [+] Less [-]Association of trypanosomosis risk with dairy cattle production in western Kenya
2005
Mugunieri, G.L. | Matete, G.O. (Trypanosomosis Research Center, Kikuyu (Kenya))
Evaluation of isometamidium levels in the serum of sheep and goats after prophylactic treatment against trypanosomosis
2004
Wesongah, J.O. | Murilla, G.A. | Kibugu, J.K. (Kenya Trypanosomiasis Research Inst., Kikuyu (Kenya)) | Jones, T.W.
Occurrence of concurrent trypanosomosis, theileriosis, anaplasmosis and helminthosis in Friesian, Zebu and Sahiwal cattle in Uganda
2002
Magona, J.W. | Mayende, J.S.P. (Livestock Health Research Inst., Tororo (Uganda))
A review of the infectious diseases of African wild ruminants
2001
Worthington, R.W. | Bigalke, R.D.
The parasitological and serological prevalence of tsetse-transmitted bovine trypanosomosis in the Eastern Caprivi (Caprivi District, Namibia)
1999
Van den Bossche, P. | Mudenge, D. (Regional Tsetse and Trypanosomosis Control Programme, Harare (Zimbabwe)) | Mubanga, J. | Norval, A.
The control of Glossina morsitans morsitans (Diptera: Glossinidae) in a settled area in Petauke District (Eastern Province, Zambia) using odour-baited targets
1997
Van den Bossche, P. (Regional Tsetse and Trypanosomosis Control Programme, Harare (Zimbabwe))
Seasonal prevalence of bovine trypanosomosis in a tsetse-infested zone and a tsetse-free zone of the Amhara Region, north-west Ethiopia
Cherenet, T.(Malaysia Putra Univ., Serdang (Malaysia). Veterinary Medicine Faculty) | Sani, R.A. | Panandam, J.M. | Nadzr, S. | Speybroeck, N. | Van den Bossche, P.
A comparative study in the clinical, parasitological and molecular diagnosis of bovine trypanosomosis in Uganda
2003
Magona, J.W. (Glasgow Univ. (United Kingdom). Veterinary School) | Mayende, J.S.P. | Olaho-Mukani, W. | Coleman, P.G. | Jonsson, N.N. | Welburn, S.C. | Eisler, M.C.
Non-immune control of trypanosomosis: In vitro oxidative burst of PMA- and trypanosome-stimulated neutrophils of Boran and N'Dama cattle
2002
Taiwo, V.O. (Ibadan Univ. (Nigeria). Veterinary Pathology Dept.) | Adejinmi, J.O. | Oluwaniyi, J.O.
