The purpose of this study was to determine the effects of vitamin C supplementation on blood oxidative stress biomarkers and antibody response to vaccination in calves.

Mastitis affects a high proportion of dairy cows throughout the world and is one of the greater problems faced by the dairy industry today. The disease is still a major cause of economic loss on a dairy farm. Mastitis poses not only negative consequences for the dairy farmer but also for the dairy industry as a number of issues threaten the reputation of milk as a healthy product from healthy animals. The use of antimicrobials is one of those concerns and threats. Antimicrobial usage on dairy farms is most often related to udder health as most medicines are used in prevention and control of mastitis. Antimicrobials remain vital for treatment of bacterial infections in dairy cattle, but in light of the upcoming debate instigated by the potential link between the use of antimicrobial products in animal husbandry and the development of antimicrobial resistance in both animal and human pathogens, there is an urgent need for innovation and alternatives to antibiotic therapy for mastitis treatment and control. Alternative approaches include vaccination, probiotics or beneficial microorganisms and inhibitory substances, immunomodulation, bacteriophages, homeopathy, and plant-derived inhibitory substances, yet only when scientifically-proven evidence is available indicating these alternatives are effective.

The effect of rabies infection and vaccination on pregnancy was investigated in different groups of pregnant rats as an animal model. Intracerebral and intramuscular experimental infection with CVS rabies virus strain was applied on four pregnant rats groups at the middle (seven days after mating) and late stages of gestation (14 days after mating). Subcutaneous rout vaccination of other three pregnant rat groups five to seven days before; seven and 14 days after mating with the inactivated cell culture local rabies vaccine. Each group of infected rats showed clinical signs of rabies although their fetuses did not show any abnormalities. Virus recovery from the placenta and fetuses from dead and sacrificed animals failed to induce rabies signs in mice inoculated intracerebrally with placenta and fetus suspensions while brains of infected dams; through the routes; revealed positive FA by using fluorescent antibody technique. Vaccinated pregnant rats did not show any abnormalities with normal fetuses and good levels of specific rabies antibodies when estimated by serum neutralization test. These findings indicate that rabies vaccination of pregnant animals is safe and it could be recommended to protect both of dams and their offspring in the first months.

Two hundred, five-week old chickens were divided into six equal groups (30 chicks/group) and vaccinated with different live NDV vaccines in Nigella sativa oil. The vaccinated birds were bled at one-week interval post-vaccination over six weeks and the collected sera were tested by the HI test. Half of each group was challenged 21-days post-vaccination by intramuscular route of inoculation with 2X105EID50 of the velogenic viscerotropic Newcastle disease virus (VVNDV). Birds were observed for 15 days post-challenge for any clinical signs of Newcastle disease. Deaths within this period were subjected to post mortem examination. The remaining birds in each group were observed after challenge till 42-days post-vaccination.

Newcastle Disease virus (NDV) and Avian Influenza virus (AI) are represent a great negative significant causing severe economic losses and increased mortalities worldwide. Newcastle disease (ND) and Avian Influenza (AI) vaccination were targeting to lower the losses from mortality, reduce the viral load in the environment as well as eradication of positive cases. Many immunostimulants had been used to improve the immune response of vaccinated chickens. The current study was designed to compare the effect of different immunostimulants on chick's immune response to bivalent ND with AI-H5N1 oil vaccine. One hundred and ten, 1- day old Baladi chicks, At the 1st day of life (0 day) 10 birds were sacrificed to obtained individual blood samples for serum to determine maternal antibodies (MDAbs) to both AI and ND. Rest of birds (100 chicks) were divided into 5 equal groups (1-5); each 20 chicks. All chicken groups were vaccinated against ND with eye drop instillation of HB1 vaccine. While, at the 9th day birds of the groups 1-3 and 5 were given H5N1 vaccine by S.C injection, birds of group 4 were lifted as non AI vaccinated control. The used immune stimulants under test were given to groups 1, 2, and 3 as follows Lector, Superimmune and Imuvral; respectively. All the groups were subjected to daily observation with recording of feed intake, weekly body weight gain and total FCR, Weekly serum samples were collected, for serological examination, and the results showed high antibody titers, low mortality rates and better body performance in the groups treated with immunostimulants than the other groups which were not treated with the immunostimulants

Assessment of the neutralizing antibody response to the attenuated strain of peste des petits ruminants Nigerian strain (75/1) in 5 pregnant ewes revealed a geometric mean titre of 194, four weeks post Vaccination. Such an appreciably high titre was found to be dropped to 73.5, throughout 48 hours post parturn. Suckling kids born to these dams passively acquired a titre of 84.5 throughout their first month of life that dropped to 48.5 at the age of 2 months. At their 5th month of age their immune titre was found to be only 4. Data generated from this study might beof value in launching vaccination campaigns against peste des petits ruminants disease.

This study was conducted to investigate the efficacy of the Newcastle disease (ND) live vaccines from different commercial sources used in different programs for vaccination of chicks having maternal antibodies against Mycoplasma infection. The immune response was estimated using HI and challenge tests. The effect on the chicken performance was estimated by the detection of the body weight gain. The role of vaccines in stimulating respiratory bacterial stress was pointed out by the lesion scores. Birds vaccinated with live vaccines from source (2) showed higher HI titers than those vaccinated with vaccine from source (1) and birds received the 2nd vaccination from heterologous source showed lower titers than those received from homologous source. Results of the challenge test indicated that birds vaccinated with live ND vaccines from one source and those vaccinated with Hitchner B1 at 33-days of age instead of La Sota showed 100% protection rate as compared with 95 % followed by vaccination with heterologous La Sota. Birdsreceived Hitchner B1 of vaccine source (1) showed only 85% protection rate. All vaccinated chicken groups showed feed conversion rates lower than the non-vaccinated control one. Groups received Hitchner B1 as a 3rd dose of the vaccine showed higher rates than those vaccinated with La Sota. Results of the lesion score for chronic respiratory disease (CRD) in vaccinated groups with different regimes revealed that birds received ND vaccinal strains from source (1) having higher scores than those received ND vaccines from the 2nd source. Administration of Hitchner B1 vaccine at 33-days of age showed lower scores. Hitchner B1 can be recommended in vaccination of chickens derived from Mycoplasma infected hens.

Objective: The research work was conducted to determine the duration of protective efficacy and lowest immune protective titer of Salmonella bivalent vaccine containing Salmonella gallinarum and Salmonella pullorum prepared at the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) of Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.Materials and methods: The experimental chickens were subdivided into four main groups (A, B, C and D). Group A and B were vaccinated with BAU-Salmonella bivalent vaccine with dosed 0.5 mL intramuscularly at the age of seven weeks followed by a booster vaccination at 12 weeks of age while group C and D served as unvaccinated control. The sera samples were obtained at 7, 12, 15, 18, 23, 27, 30, 32, 34, 36 and 41 weeks of age of birds. Results: Significantly elevated level of immune response in terms of antibody production resulted from booster vaccination. Vaccinated chicken showed protective resistance following virulent challenge with isolates of S. gallinarum and S. pullorum (**P [J Adv Vet Anim Res 2018; 5(3.000): 282-289]

The objective of this project was to develop live (LV), live attenuated (LAV), and autoclaved killed vaccines (AKV). The development of the vaccine involves utilizing two well-characterized strains of Streptococcus iniae (S. iniae), namely S. iniae 1 and S. iniae 2. S. iniae 2 was obtained from Department of Fish diseases, Faculty of Veterinary Medicine, Beni-Suef University, Egypt while S. iniae 1 strain was gifted from microbiology department, Egyptian Drug Authority, Dokki, Giza, Egypt. Pathogenicity test and lethal dose determination were performed. To conduct the experiment, apparently healthy Nile tilapia, Oreochromis niloticus (O. niloticus) of average weight 30±0.2g were divided into five experimental groups: T1 group, which served as a negative control and received saline; T2 group, which served as a positive control and received S. iniae 2; T3 group, which received an autoclaved killed vaccine for S. iniae 2; T4 group, which received a live attenuated vaccine for S. iniae 2; and T5 group, which received a live vaccine for S. iniae 1. At the end of the vaccination period, S. iniae 2 was introduced challenge to all groups. Serum samples were collected three weeks after vaccination to measure serum bactericidal activity, lysozyme activity, nitric oxide, alkaline phosphatase, and acid phosphatase. The findings demonstrated that the pathogenicity test reach 0 and 100% mortality rate for S. iniae 1 and S. iniae 2, respectively. Live attenuated vaccine had significantly higher protective rate than live vaccinations, while autoclaved vaccine had the best protective efficacy (88.2%). These results were confirmed through measurement some immune parameters as Serum bactericidal activity, lysozyme activity, nitric oxide, alkaline phosphatase and acid phosphatase.

Human rabies transmitted by dogs still kills thousands of people each year worldwide. Dog bites are common in the city of Beni (Democratic Republic of Congo), which shows low rabies vaccination coverage. This study aimed to determine the factors associated with the rabies vaccination status of dogs. A cross-sectional analytical study was conducted in the town of Beni among dog owners, during a household survey selected using a multistage sampling. The information sought concerned the knowledge and characteristics of the dog owners as well as the vaccination status of these dogs. Logistic regression was used to investigate associations between the vaccination status of the dogs and the main independent factors. Rabies vaccination coverage in Beni was 26% (95% confidence interval [CI]: 22% – 30%). The main factors associated with the rabies vaccination status of the dog were primary education level of household head (adjusted odds ratio [aOR]:4.8; 95% CI: 1.2– 19.8); university education level of household head (aOR: 5.9; 95% CI: 1.6–22); perceived rabies severity (aOR: 44. 4; 95% CI: 10.4–188), having more than one dog in the household (aOR: 2.6; 95% CI: 1.6–4.3); age range 7–12 months (aOR: 0.2; 95% CI: 0.1–0.6) and confined dog breeding (aOR: 3.9; 95% CI: 1.1–14.9). The low vaccination coverage in Beni requires mass vaccination campaigns against canine rabies targeting the dog owners with low education levels, those raising more than one dog, with stray dogs or dogs less than 12 months old.