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Attempted transmission of Ehrlichia canis by Rhipicephalus sanguineus after passage in cell culture.
1996
Mathew J.S. | Ewing S.A. | Barker R.W. | Fox J.C. | Dawson J.E. | Warner C.K. | Murphy G.L. | Kocan K.M.
The historical background and global importance of heartwater
1987
Provost, Alain | Bezuidenhout, J.D.
Establishment of a laboratory colony of taiga tick Ixodes persulcatus for tick-borne pathogen transmission studies
2008
Konnai, S.(Hokkaido Univ., Sapporo (Japan)) | Saito, Y. | Nishikado, H. | Yamada, S. | Imamura, S. | Mori, A. | Ito, T. | Onuma, M. | Ohashi, K.
Ixodes persulcatus Schulze (I. persulcatus) is distributed in Russia and Far East Asia including Japan, and has been implicated as the vector of several human pathogens. In particular, I. persulcatus acts as the only tick vector for human lyme borreliosis in Japan. In order to elucidate the mechanism of transmission of I. persulcatus-borne pathogens, we developed a laboratory colony of I. persulcatus. Ticks were fed on Syrian hamster and engorged ticks that had dropped off the animals were collected and maintained to allow them to molt. Tick rearing was performed in incubator at 20degC with 95% relative humidity and 12-hour light/dark photo-period regimen. We found out that adult females fed for 8+-2 days and had a pre-oviposition period lasting for 7+-2 days. The minimum egg incubation period was 1 month with the hatched larvae feeding for 3+-1 days and molting to nymphs 3-4 months thereafter. Meanwhile, the nymphs fed for 4+-1 days and molted to adult 2-3 months thereafter. For future analysis of gene expression profiles in I. persulcatus, we cloned and sequenced the actin gene (a housekeeping gene), and found that it is 92.7% to 98.6% homologous to the published sequences of related ixodid ticks. This laboratory colony of I. persulcatus will facilitate investigations on the role of tick-derived molecules on the transmission of I. persulcatus-borne pathogens and will be important for identification of potential anti-tick vaccine and acaricide target molecules.
Show more [+] Less [-]Geographical distribution and relative abundance of stock-associated Culicoides species (Diptera: Ceratopogonidae) in southern Africa, in relation to their potential as viral vectors
1996
Venter, G.J. | Nevill, E.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Van der Linde, T.C. de K.
African horsesickness epidemiology: five species of Culicoides (Diptera: Ceratopogonidae) collected live behind the ears and at the dung of the African elephant in the Kruger National Park, South Africa
1994
Meiswinkel, R. (National Parks Board, Skukuza (South Africa)) | Braack, L.E.O.
Culicoides (Diptera: Ceratopogonidae) associated with livestock in the Onderstepoort area, Gauteng, South Africa as determined by light-trap collections
1996
Venter, G.J. | Meiswinkel, R. | Nevill, E.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Edwardes, M.
The virtual absence of Culicoides imicola (Diptera: Ceratopogonidae) in a light-trap survey of the colder, higher-lying area of the eastern Orange Free State, South Africa, and implications for the transmission of arboviruses
1994
Venter, G.J. | Meiswinkel, R. (Onderstepoort Veterinary Inst. (South Africa). Entomology Section)
