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Bacterial contaminants in extended boar semen and selection of effective antimicrobials
2010
Kim, H.Y., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Byun, J.W., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Shin, D.H., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, H.S., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Yoon, H.C., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Park, C.K., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lee, O.S., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Jung, B.Y., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea
Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups (less-than or equal to 24 and greater than 24 month old). Stenotrophomonas maltophilia (n=18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n=12), phingomonas paucimobilis (n=12), Myroides spp. (n=5), Ochrobactrum anthropi (n=3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.
Show more [+] Less [-]Влияние натрия тиосульфата на морфологию костного мозга птиц при ассоциированной вакцинации против вирусных болезней
2009
Gromov, I.N. | Prudnikov, V.S., Vitebsk State Academy of Veterinary Medicine (Belarus) | Birman, B.Ya., National Academy of Sciences. Scientific and Practical Center of Animal Breding (Belarus). The S.N. Vyshelesskij Inst. of Experimental Veterinary Medicine
Investigation of morphological changes in bone marrow of young hens in the conditions of application of associated virus vaccines and sodium thiosulphate immunostimulant was realized in the Republic of Belarus. The associated virus vaccine was applied against infectious bursal disease, infectious bronchitis, infectious laryngotracheitis, and Newcastle disease. Research results showed that under the influence of inactivated vaccine against mentioned above diseases there was noted the increasing of quantity of granulocytic cells, there was stated an active hyperplasia of pseudoeosinophil cells. Immunization of young hens with the presented vaccine developed in the Institute of Experimental Veterinary Medicine of the National Academy of Sciences of Belarus promoted a short-term increasing of leucoerythroblastic index. Application of an alternative vaccine developed by Scientific and Production Enterprise Avivak did not render substantial influence on the morphology of bone marrow of chickens. Sodium thiosulphate applied in combination with associated vaccines (in 7% concentration in vaccine) caused the hyperplasia of lymphoid and thrombocyte cells, strengthened the proliferation and maturity of granular leukocytes
Show more [+] Less [-]Полиморфизм гена каппа-казеина (CSN3) в популяции крупного рогатого скота белоруской черно-пестрой породы
2009
Yatsyna, O.A. | Smuneva, V.K., Vitebsk State Academy of Veterinary Medicine (Belarus) | Epishko, T.I., Polesje State Univ., Pinsk (Belarus)
Determination of genetic structure of different population lines of Belarusian white-and-black breed in accordance with locus of kappa-casein gene (CSN3) was realized in the conditions of the Republic of Belarus. In course of study there were used DNA samples of Belarusian white-and-black breed cows (380 samples) and bioprobes of servicing bulls (83 samples). With the help of endonuclease Hindill there were revealed two alleles of kappa-casein (A and B) in DNA preparations. As a result of genetic typing of breeding animals by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods there were revealed three genotypes CSN3**AA, CSN3**AB, and CSN3**BB. The frequency of occurrence of desirable genotype CSN3**BB of Belarusian white-and-black breed of cows depending on breed and linear pertain varied from 2,2% up to 7,69%, for servicing bulls – from 0 up to 11,1% . The majority of homozygotic cows with undesirable cow genotype CSN3**AA belonged to the following lines: of Ruties Edward (2,31646 - 92,3%), Niko (86,0%), Vis Ajdal (933122 - 71,9%), Reflection Sovereign (198998 – 63,9%). The desirable CSN3**BB genotype in most of the cases was stated at Annas-Adema (30587) and Hiltes-Adema (37910) lines. Presence of heterozygote genotype predominated in Annas-Adema (46,1%) and Montvik Chiftein (36,3%) lines. Realized analysis of cow population lines of Danish and Holstein breeds showed that the average frequency of occurrence of CSN3**B allel was 17%, but lower concentration (15%) of CSN3**b allel was typical for the Danish breeds. Frequency of occurrence of CSN3**B allel in Holsten lines was 18%. In was revealed that in 86,6% of cases bulls were the genetic carriers of CSN3**A allel and only 11,4% of animals had the desirable allel CSN3**B in their genotype
Show more [+] Less [-]Влияние импортных хряков породы ландрас на откормочную и мясную продуктивность животных белорусской мясной породы
2008
Shejko, I.P. | Fedorenkova, L.A. | Yanovich, E.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Influence of 33 imported heads Landrace breed boars of foreign selection on feeding performance and meat productivity of swine of the Belarusian Meaty breed was studied in the conditions of the Republic of Belarus for the enhancement of Belarusian breeds genealogical structure. There was stated the positive influence of Landrace Breed on feeding performance and meat productivity of mixed bred stores obtained after the introductive crossing with sows of Belarusian Meaty breed which was expressed in the predominancy of mixed bred hogs over the purebred ones at the stage of fattening performance test: at the age of reaching 100 kg body weight – on 0,7%; at average daily weight gain - on 1,5%; at feed consumption - on 1,2%. Mixed bred stores also had thinner back fat (on 0,9 mm) and bigger loin (on 0,4 square cm). There was stated the one-sided influence of boars of Landrace Breed on feeding performance and meat productivity indices of mixed breeds. It was established, that hybrids of four lines (Zaboj, Zalet, Zont and Bison) with regard to control groups showed the effect of heterosis at age of achievement of body weight of 100 kg, at a daily average weight gain and at forage consumption in the following rates: 1,5% (Р less than 0,05), 2,8% and 1,5% (Р less than 0,05) for Zaboj line; 2,3%, 4,9% and 2,1% (Р less than 0,01) for Zalet lines; 1,4%, 3% and 1,5% (Р less than 0,05) for Zont lines; 3% (Р less than 0,001), 6,7% and 2,7% for a line of Bison. In meat productivity indices there was stated the in-between character of inheritance which caused the decreasing of fat thickness and increasing of loin eye area at descendants of trial groups of lines: Zaboj, Zalet, Zenit, Zont on 0,88 mm (Р less than 0,05) and 0,6 square cm, 1,7 mm (Р less than 0,01) and 2,1 square cm (Р less than 0,001), 1,3 mm (Р less than 0,01) and 0,9 square cm, 1,4 mm
Show more [+] Less [-]Изменение гематологических показателей у хряков при стимуляции биологически активных точек
2008
Elisejkin, D.V. | Sobolev, D.T. | Levkin, E.A., Vitebsk State Academy of Veterinary Medicine (Belarus)
There was studied the changes of the general protein content, albuminous fractions, antibodies and leukogram in blood of boars at influence by the laser on biologically active points. At laser influence on biologically active points, which are situated in lumber and sacral section spine, is taken place stimulating of body resistance. In the experiences the influence by the laser with various frequency has led to authentic increase in the content of protein in blood at 512 Hz with 75,9 to 81,2 and at 4046 with 75,4 to 80,8 g/l. The percent of eosinocytes in blood which carry out antitoxic function increases - from 2,63 to 4,85% and according to 2,50 to 5,15% at P0,01. Influence by laser radiation has caused number increase limfotsits in blood from 44,38 to 50,13% and according from 45,38 to 49,38% at P0,01. In the research the analysis of the content of antibodies in blood of animals was carried out. It was established that after processing by the laser (P0,05) the content in blood of immunoglobulin A increases at frequency of influence of 512 Hz from 2,2 to 3,2 g/l and at frequency of 4046 Hz from 2,4 to 3,3 g/l. | Цель работы заключалась в изучении изменения содержания общего белка, белковых фракции, иммуноглобулинов и лейкограммы в крови хряков при воздействии лазером на биологически активные точки. При воздействии лазером на биологически активные точки, расположенные в поясничном и крестцовом отделе позвоночника, происходит стимуляция резистентности организма. В проведенных опытах воздействие лазером с различной частотой привело к достоверному увеличению содержания белка в крови при 512 Гц с 75,9 до 81,2 и при 4046 с 75,4 до 80,8 г/л. Процент эозинофилов в крови, которые выполняют антитоксическую функцию, увеличивается с 2,63 до 4,85% и соответственно с 2,50 до 5,15% при Р0,01. Воздействие лазерным излучением вызвало увеличение числа лимфоцитов в крови с 44,38 до 50,13% и соответственно с 45,38 до 49,38% при Р0,01. В исследованиях проведен анализ содержания иммуноглобулинов в крови животных. Установлено, что после обработки лазером достоверно (Р0,05) увеличивается содержание в крови иммуноглобулина А при частоте воздействия 512 Гц с 2,2 до 3,2 г/л и при частоте 4046 Гц с 2,4 до 3,3 г/л.
Show more [+] Less [-]Новые линии в белорусской крупной белой породе свиней
2009
Loban, N.A. | Vasilyuk, O.Ya. | Chernov, A.S. | Asomchik, N.V., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
In course of a long-term selection breeding activities in the conditions of the Republic of Belarus on the basis of Belarusian Large White breed of swine there were created two high-productive lines - Delfina 37755 and Svitanka 3884. Individual selection of animals was realized in accordance with the main indexes of productive ability, development and conformation type. There were selected parent lines, there were used methods of intrastrain breeding and hybrid between lines, mild inbreeding on parental line. Bores and breeding sows were evaluated in accordance with their posterity productivity by means of a control feeding method. Productive indexes for Delfina 37755 line were 11,7 piglets, for Svitanka 3884 line – 11,9 piglets; age of live weight gain of 100 kg – 189 and 182 days, respectively; average daily weight gain – 735 gram and 749 gram; forage expenses – 3,5 and 3,51 fodder units; backfat thickness – 27,2 and 26,2 mm; ham weight – 10,9 and 11,2 k, respectively
Show more [+] Less [-]Влияние генотипа хряков по генам EPOR, MUC4 и ICF-2 на продуктивность потомства
2009
Dojlidov, V.A. | Kaspirovich, D.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.I. | Mikhajlova, T.I., Selection and Hybrid Centre Zadneprovski (Belarus)
During the investigation of nuclear DNA of boas of Belarusian meaty breed there was studied the polymorphism of EPOR, MUC4 and IGF-2 genes with the further determination of frequency of alleles and genotypes. Among boars tested for the polymorphism EPOR gene there was stated high percentage of heterozygous samples (EPOR**st): 43,3% for Belarusian meaty breed boars and 66,7% for Belarusian Kripnaya Belaya breed. In course of analysis of the genetic structure by MUC4 gene there was revealed the predominance of animals with the desirable d allele combination (MUC**cc): 60% for Belarusian Kripnaya Belaya breed and 82,2% for Belarusian meaty breed; by IGF-2 gene with homozygotic manifestation of recessive alleles: 46,4% and 63,4%, respectively. In the conditions of the Republic of Belarus there were determined the preferred boar genotypes with EPOR, MUC4 and ICF-2 genes and combinations which made it possible to increase the fattening and meaty qualities of posterity. The research results showed that in the selection breeding schemes it was necessary to consider not only maternal genotype, but also male genotypes giving preference to EPOR**st, MUC4**ss and ICF-2**qq genotypes
Show more [+] Less [-]Продуктивность свиней в зависимости от продолжительности их внутриутробного развития
2009
Pinchuk, V.F., Combine State Farm Voskhod, Mogilev region (Belarus)
In the conditions of the swine breeding complex in the Republic of Belarus where had been applied the three-way crossing and self-replacement of swine herd there was studied the productivity of swine depending on duration of their embryonic development. There was developed new method of selection replacement gilts which made it possible to select a breeding swine flock with high adaptation abilities to the industrial housing conditions. Research results showed that the duration of pregnancy in the conditions of three-way rotational crossbreeding and self-replacement of swine herd was 114,5 +/- 0,03 days with variance between 103 and 121 days. In the majority of breeding sows (93%) the duration of pregnancy period was within the limits from 112 up to 117 days. Season of a year rendered influence on the duration of sow pregnancy: the longest one was in winter and spring, the shortest – in summer and autumn. With advancing age there was stated the increased duration of pregnancy period. Difference in duration for first-pregnant sows and sows with 8 farrows was 0,51 days. With increase of pregnancy duration there was noted the lowering of multiple fetation, but there was the increasing of live weight of piglets at birth, their growth energy and viability
Show more [+] Less [-]Генетический профиль хряков плановых пород по гену IGF-2 и его влияние на мясо-откормочные качества потомства
2009
Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
The influence of polymorphism of gene IGF-2 on feeding and meat qualities of pigs of Belarusian Large White breed was studied. It is established, that frequency of presumptive preferable allele Q gene IGF-2 reaches 0,75-0,98% at boars of meat breeds and 0,34% - at Belarusian Large White breed. The young boars of breed with genotype QQ authentically surpass the analogues with a genotype qq on development, and feeding young pigs – on meat-feeding to qualities. Gene IGF-2 it is possible to consider as genetic marker of growth rate of pigs of the Belarusian Large White breed
Show more [+] Less [-]Влияние уровня селена на продуктивность хряков-производителей
2008
Poznyak, V.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
Determination of the optimal doses of selenium inclusion into premix structure for breeding boars for the maintenance of their higher producing capacity was realized in the conditions of the Republic of Belarus. Selenium lack in boar rations caused the degenerative changes in seminal glands, low male germ cells concentration and lowering of spermatozoon motility, which subsequently decreased their producing efficiency. Research showed, that selenium inclusion in premix KS-1 structure in quantity of 30 mg per 1 kg in comparison with premix in 20 mg dose have improved the yielding productivity of mature boars: the average quantity of male germ cells per one ejaculate increased on 17,2%, the average quantity of born pigs from per one sow increased on 4,8%. The additional profit per one boar was on 6,06% higher than in control experiment
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