A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield. Clinical examination of udder revealed normal milk color and soft udder. The milk pH was 7.0, with California Mastitis Test score 3+, Electrical Conductivity 270U, and Somatic Cell Count as 328,000. Isolation and identification of causative agent revealed Methicillin Resistant Staphylococcus aureus (MRSA) from the sub-acute mastitis sample. Agar disc diffusion method for antimicrobial susceptibility revealed that the MRSA was sensitive to Enrofloxacin, Gentamicin, Oxytetracycline and Amoxicillin+Sulbactam. On the other hand, the isolate was resistance to Amoxicillin, Penicillin G, Ceftriaxone and Methicillin. The isolate was positive for ?-lactamase resistance by Nitrocefin test. The MRSA was confirmed for the presence of mecA and blaZ target genes by polymerase chain reaction (PCR). The cow was treated with Enrofloxacin, Vitamin E and inorganic Selenium, and was recovered after 5 days of post-treatment. http://dx.doi.org/10.5455/javar.2014.a35

A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield. Clinical examination of udder revealed normal milk color and soft udder. The milk pH was 7.0, with California Mastitis Test score 3+, Electrical Conductivity 270U, and Somatic Cell Count as 328,000. Isolation and identification of causative agent revealed Methicillin Resistant Staphylococcus aureus (MRSA) from the sub-acute mastitis sample. Agar disc diffusion method for antimicrobial susceptibility revealed that the MRSA was sensitive to Enrofloxacin, Gentamicin, Oxytetracycline and Amoxicillin+Sulbactam. On the other hand, the isolate was resistance to Amoxicillin, Penicillin G, Ceftriaxone and Methicillin. The isolate was positive for β-lactamase resistance by Nitrocefin test. The MRSA was confirmed for the presence of mecA and blaZ target genes by polymerase chain reaction (PCR). The cow was treated with Enrofloxacin, Vitamin E and inorganic Selenium, and was recovered after 5 days of post-treatment.

Taenia saginata cysticercosis is one of the zoonotic diseases that threaten food safety and food security, particularly in developing countries. A cross-sectional study was conducted to estimate the prevalence and cyst distribution in infected cattle, and food safety implications of Taenia saginata cysticercosis in Harari People’s National Regional State, eastern Ethiopia. Post-mortem inspection of carcasses and organs of slaughtered cattle in Harar Municipal Abattoir, cyst viability tests and interviews with randomly selected meat consumers were undertaken. The post-mortem inspection showed that of the 898 local zebu cattle slaughtered for human consumption and examined for the presence of cysticerci of T. saginata, 19.7% (177/898; 95% CI = 17.2–22.5) harboured at least one cyst in the muscles or organs inspected. Of the edible anatomical sites with cysticerci, shoulder muscle, liver and heart together represented 65.4%, 66.0% and 65.4% respectively of relative prevalence, total cyst count and cyst viability. These edible sites are preferred above others by local people for preparation and consumption of raw or inadequately cooked meat dishes that are locally served as kurt, kitffo and dullet. The interviews revealed that among the 300 study participants, 182 (60.7%) had been infected by taeniosis at least once during the previous year and of these 99.0% had eaten raw or undercooked beef, the majority (88.3%) obtained from butchers assumed to provide officially inspected meat that was fit for consumption. This indicated that existing meat inspection processes were inadequate to prevent carcasses infected with T. saginata cysticerci from reaching consumers. The high prevalence of viable cysts in the edible parts of beef together with the widespread consumption of raw or undercooked beef indicated the importance of T. saginata cysticercosis as a food safety problem in eastern Ethiopia. The promotion of policies to upgrade existing meat inspection procedures and public education to ensure effective prevention of T. saginata taeniosis in humans were recommended.

Taenia saginata cysticercosis is one of the zoonotic diseases that threaten food safety and food security, particularly in developing countries. A cross-sectional study was conducted to estimate the prevalence and cyst distribution in infected cattle, and food safety implications of Taenia saginata cysticercosis in Harari People’s National Regional State, eastern Ethiopia. Post-mortem inspection of carcasses and organs of slaughtered cattle in Harar Municipal Abattoir, cyst viability tests and interviews with randomly selected meat consumers were undertaken. The post-mortem inspection showed that of the 898 local zebu cattle slaughtered for human consumption and examined for the presence of cysticerci of T. saginata, 19.7% (177/898; 95% CI = 17.2–22.5) harboured at least one cyst in the muscles or organs inspected. Of the edible anatomical sites with cysticerci, shoulder muscle, liver and heart together represented 65.4%, 66.0% and 65.4% respectively of relative prevalence, total cyst count and cyst viability. These edible sites are preferred above others by local people for preparation and consumption of raw or inadequately cooked meat dishes that are locally served as kurt, kitffo and dullet. The interviews revealed that among the 300 study participants, 182 (60.7%) had been infected by taeniosis at least once during the previous year and of these 99.0% had eaten raw or undercooked beef, the majority (88.3%) obtained from butchers assumed to provide officially inspected meat that was fit for consumption. This indicated that existing meat inspection processes were inadequate to prevent carcasses infected with T. saginata cysticerci from reaching consumers. The high prevalence of viable cysts in the edible parts of beef together with the widespread consumption of raw or undercooked beef indicated the importance of T. saginata cysticercosis as a food safety problem in eastern Ethiopia. The promotion of policies to upgrade existing meat inspection procedures and public education to ensure effective prevention of T. saginata taeniosis in humans were recommended.

In order to investigate the extent of genetic diversity among Ehrlichia ruminantium strains in Cameroon, a partial fragment (800 bp) of the E. ruminantium map1 gene was amplified by nested polymerase chain reaction in 121 of 156 E. ruminantium pCS20-positive DNA samples extracted from ticks and cattle collected from two ranches. Deoxyribonucleic acid sequencing of the map1 gene products indicated the presence of at least 21 genotypes at the nucleotide level and 16 genotypes at the amino acid level circulating within the study sites. Some of the genotypes were identical to Antigua (U50830), Blaaukrans (AF368000) or UmBanein (U50835), whilst the others were new genotypes. Twenty-four representative sequences were deposited in GenBank and given accession numbers JX477663 – JX477674 (for sequences of tick origin) and JX486788 – JX486799 (for sequences of cattle origin). Knowledge of E. ruminantium strain diversity could be important in understanding the epidemiology of heartwater

In order to investigate the extent of genetic diversity among Ehrlichia ruminantium strains in Cameroon, a partial fragment (800 bp) of the E. ruminantium map1 gene was amplified by nested polymerase chain reaction in 121 of 156 E. ruminantium pCS20-positive DNA samples extracted from ticks and cattle collected from two ranches. Deoxyribonucleic acid sequencing of the map1 gene products indicated the presence of at least 21 genotypes at the nucleotide level and 16 genotypes at the amino acid level circulating within the study sites. Some of the genotypes were identical to Antigua (U50830), Blaaukrans (AF368000) or UmBanein (U50835), whilst the others were new genotypes. Twenty-four representative sequences were deposited in GenBank and given accession numbers JX477663 – JX477674 (for sequences of tick origin) and JX486788 – JX486799 (for sequences of cattle origin). Knowledge of E. ruminantium strain diversity could be important in understanding the epidemiology of heartwater

A cross-sectional study aimed at investigating the species diversity of fly vectors and estimating the prevalence of bovine trypanosomosis was carried out from October 2009 to May 2010 in selected settlement areas of the Hawa-Gelan district in the western Wollega zone of Ethiopia. Standard methods of sampling and identification were employed for both entomological and parasitological examination. Three species of the genus Glossina (Glossina pallidipes, Glossina morsitans submorsitans and Glossina fuscipes) and two genera of biting flies (Stomoxys and Tabanus) were caught and identified. The overall apparent density of Glossina species caught was 10.5 flies per trap per day, with a higher proportion of female flies (57.2%). Out of a total 389 cattle examined, 42 (10.8%; 95% CI: 7.89% – 14.3%) were found infected with trypanosomes. Three trypanosome species were detected in the study area, namely Trypanosoma congolense (54.8%), Trypanosoma brucei (23.8%) and Trypanosoma vivax (21.4%). The prevalence of trypanosomosis was found to be significantly (p 0.05) higher in cattle with poor body condition. There was an association between mean packed cell volume (PCV) and the occurrence of parasitaemia (χ2 = 49.5, p 0.05). About 95.2% of cattle that were positive for trypanosomes had a PCV less than the lower limit for cattle. Considering the current result, bovine trypanosomosis seems to be a serious constraint for agricultural activities in the settlement areas of the Hawa-Gelan district and seems to be associated with the presence of Glossina species. Therefore, application of control methods through community involvement to reduce the Glossina species infestation level is likely to increase animal productivity.

A cross-sectional study aimed at investigating the species diversity of fly vectors and estimating the prevalence of bovine trypanosomosis was carried out from October 2009 to May 2010 in selected settlement areas of the Hawa-Gelan district in the western Wollega zone of Ethiopia. Standard methods of sampling and identification were employed for both entomological and parasitological examination. Three species of the genus Glossina (Glossina pallidipes, Glossina morsitans submorsitans and Glossina fuscipes) and two genera of biting flies (Stomoxys and Tabanus) were caught and identified. The overall apparent density of Glossina species caught was 10.5 flies per trap per day, with a higher proportion of female flies (57.2%). Out of a total 389 cattle examined, 42 (10.8%; 95% CI: 7.89% – 14.3%) were found infected with trypanosomes. Three trypanosome species were detected in the study area, namely Trypanosoma congolense (54.8%), Trypanosoma brucei (23.8%) and Trypanosoma vivax (21.4%). The prevalence of trypanosomosis was found to be significantly (p < 0.05) higher in cattle with poor body condition. There was an association between mean packed cell volume (PCV) and the occurrence of parasitaemia (χ2 = 49.5, p < 0.05). About 95.2% of cattle that were positive for trypanosomes had a PCV less than the lower limit for cattle. Considering the current result, bovine trypanosomosis seems to be a serious constraint for agricultural activities in the settlement areas of the Hawa-Gelan district and seems to be associated with the presence of Glossina species. Therefore, application of control methods through community involvement to reduce the Glossina species infestation level is likely to increase animal productivity.

Limpopo is a very important area for pig production in terms of animal populations and contributions to transboundary animal disease spread. Emerging small-scale pig farmers (ESSPF) are being encouraged to establish operations and spread in South Africa; however, for these farmers to perform optimally, they need to understand the basics of animal agriculture and contribute to enhancing biosecurity and efficient production systems. In the present study, the limitations to efficient production amongst ESSPF were evaluated and some improvements were suggested. It was found that the ESSPF are dominated by males and include a large percentage of older persons. A total of 26.54% of these farmers have post-matriculation qualifications. Undefined and indigenous breeds still dominate their animal genetics. The animal health technicians are the preferred channels by which farmers report diseases to the authorities (52.47%) and only one out of five (20.37%) will preferably report a disease situation direct to a veterinarian. These farmers do not vaccinate their stock, and knowledge of biosecurity is poor. Antimicrobials, especially tetracyclines, are abused. Animals that are slaughtered within the community or sold at local sale points, pension pay stations and auction markets are likely candidates for disease spread. It is recommended that the younger generations are retained and incentivised in animal agriculture. Improved training on management, health, biosecurity and better market access must be provided for the ESSPF, whilst efforts should made to consolidate these farmers into small cooperatives. The current government agricultural support system will need to be reworked to benefit the resource-poor farmers. Collaborative efforts in disease reporting and management among veterinarians, animal health technicians and extension officers will become necessary. Finally, the creation of a progressive quality grading system for ESSPF should be planned by the industry and this should be attached to a reward system that will encourage these farmers to target good farming practice.

Limpopo is a very important area for pig production in terms of animal populations and contributions to transboundary animal disease spread. Emerging small-scale pig farmers (ESSPF) are being encouraged to establish operations and spread in South Africa; however, for these farmers to perform optimally, they need to understand the basics of animal agriculture and contribute to enhancing biosecurity and efficient production systems. In the present study, the limitations to efficient production amongst ESSPF were evaluated and some improvements were suggested. It was found that the ESSPF are dominated by males and include a large percentage of older persons. A total of 26.54% of these farmers have post-matriculation qualifications. Undefined and indigenous breeds still dominate their animal genetics. The animal health technicians are the preferred channels by which farmers report diseases to the authorities (52.47%) and only one out of five (20.37%) will preferably report a disease situation direct to a veterinarian. These farmers do not vaccinate their stock, and knowledge of biosecurity is poor. Antimicrobials, especially tetracyclines, are abused. Animals that are slaughtered within the community or sold at local sale points, pension pay stations and auction markets are likely candidates for disease spread. It is recommended that the younger generations are retained and incentivised in animal agriculture. Improved training on management, health, biosecurity and better market access must be provided for the ESSPF, whilst efforts should made to consolidate these farmers into small cooperatives. The current government agricultural support system will need to be reworked to benefit the resource-poor farmers. Collaborative efforts in disease reporting and management among veterinarians, animal health technicians and extension officers will become necessary. Finally, the creation of a progressive quality grading system for ESSPF should be planned by the industry and this should be attached to a reward system that will encourage these farmers to target good farming practice.

With the extensive use of antibiotics in livestock production, surveillance revealed an increase in Salmonella resistance to the commonly used antimicrobials in veterinary and public health. This serious threat to health care is further exacerbated by the limited epidemiological information about the common zoonotic agent, Salmonella enteritidis, required to determine antibiotic therapy. The aim was to characterise the antimicrobial resistance patterns of S. enteritidis isolates across different timelines (1972–2005) with accompanying genetic changes being investigated. Thirty-seven stored S. enteritidis isolates were collected from the Central Veterinary Laboratory, Harare, with antimicrobial susceptibility determined against eight antibiotics. Plasmids were isolated to analyse any genetic variation. An overall significant increase in resistance (p 0.05) to nalidixic acid (0% – 10%), ampicillin (14.3% – 50%), tetracycline (14.3% – 30%) and erythromycin (71.4% – 100%) was observed across the timeline. However, the highest rates of susceptibility were maintained for gentamicin, sulphamethoxazole-trimethoprim, kanamycin and chloramphenicol. We report an increase in multidrug resistance (MDR) of 14.2% – 50% with an increase in resistotypes and plasmid profiles across the timeline. Eleven plasmid profiles were obtained in the 37 isolates studied with a minority of isolates (21.6%, 8/37) harbouring a 54 kb plasmid, commonly serovar-specific. A concerning increase in antimicrobial resistance to commonly administered drugs was observed across the timeline. The surge in MDR is of great concern and implies the need for consistent antimicrobial stewardship. No correlation was observed between the plasmid and antibiotic profiles.

With the extensive use of antibiotics in livestock production, surveillance revealed an increase in Salmonella resistance to the commonly used antimicrobials in veterinary and public health. This serious threat to health care is further exacerbated by the limited epidemiological information about the common zoonotic agent, Salmonella enteritidis, required to determine antibiotic therapy. The aim was to characterise the antimicrobial resistance patterns of S. enteritidis isolates across different timelines (1972–2005) with accompanying genetic changes being investigated. Thirty-seven stored S. enteritidis isolates were collected from the Central Veterinary Laboratory, Harare, with antimicrobial susceptibility determined against eight antibiotics. Plasmids were isolated to analyse any genetic variation. An overall significant increase in resistance (p < 0.05) to nalidixic acid (0% – 10%), ampicillin (14.3% – 50%), tetracycline (14.3% – 30%) and erythromycin (71.4% – 100%) was observed across the timeline. However, the highest rates of susceptibility were maintained for gentamicin, sulphamethoxazole-trimethoprim, kanamycin and chloramphenicol. We report an increase in multidrug resistance (MDR) of 14.2% – 50% with an increase in resistotypes and plasmid profiles across the timeline. Eleven plasmid profiles were obtained in the 37 isolates studied with a minority of isolates (21.6%, 8/37) harbouring a 54 kb plasmid, commonly serovar-specific. A concerning increase in antimicrobial resistance to commonly administered drugs was observed across the timeline. The surge in MDR is of great concern and implies the need for consistent antimicrobial stewardship. No correlation was observed between the plasmid and antibiotic profiles.

Kikuyu poisoning occurs sporadically in South Africa. It is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. Pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the Eastern Cape Province of South Africa from 2008 to 2010. The kikuyu grass samples were submitted specifically for the isolation and molecular identification of Fusarium species, as it was recently suggested that mycotoxins synthesised by Fusarium torulosum could be the cause of this intoxication. Ninety-four Fusarium isolates were retrieved from the grass samples, of which 72 were members of the Fusarium incarnatum/Fusarium equiseti species complex based on morphology and phylogenetic analyses of the translation elongation factor 1α sequence data. The South African isolates from kikuyu identified as members of the F. incarnatum/F. equiseti species complex grouped together in six separate clades. The other isolates were Fusarium culmorum (n = 3), Fusarium redolens (n = 4) and Fusarium oxysporum (n = 15). Although F. torulosum could not be isolated from P. clandestinum collected during kikuyu poisoning outbreaks in South Africa, the mycotoxicosis theory is still highly plausible.

Kikuyu poisoning occurs sporadically in South Africa. It is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. Pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the Eastern Cape Province of South Africa from 2008 to 2010. The kikuyu grass samples were submitted specifically for the isolation and molecular identification of Fusarium species, as it was recently suggested that mycotoxins synthesised by Fusarium torulosum could be the cause of this intoxication. Ninety-four Fusarium isolates were retrieved from the grass samples, of which 72 were members of the Fusarium incarnatum/Fusarium equiseti species complex based on morphology and phylogenetic analyses of the translation elongation factor 1α sequence data. The South African isolates from kikuyu identified as members of the F. incarnatum/F. equiseti species complex grouped together in six separate clades. The other isolates were Fusarium culmorum (n = 3), Fusarium redolens (n = 4) and Fusarium oxysporum (n = 15). Although F. torulosum could not be isolated from P. clandestinum collected during kikuyu poisoning outbreaks in South Africa, the mycotoxicosis theory is still highly plausible.

This review examined the use of green tea (Camellia sinensis) in the diets of poultry. Research findings were obtained from various recent studies, where much attention was focused on the role of green tea in the promotion of both animal and human health. The review involved some of the currently available information about green tea, pertaining to its chemical composition, anticoccidial and antimicrobial effect, effect on broiler and layer performance and on blood and egg yolk constituents. To the author’s knowledge this is the first review paper on this topic. It will be helpful for poultry nutritionists and the poultry industry, although more detailed studies are still needed to elucidate the effects of green tea in poultry nutrition under various circumstances.

This review examined the use of green tea (Camellia sinensis) in the diets of poultry. Research findings were obtained from various recent studies, where much attention was focused on the role of green tea in the promotion of both animal and human health. The review involved some of the currently available information about green tea, pertaining to its chemical composition, anticoccidial and antimicrobial effect, effect on broiler and layer performance and on blood and egg yolk constituents. To the author’s knowledge this is the first review paper on this topic. It will be helpful for poultry nutritionists and the poultry industry, although more detailed studies are still needed to elucidate the effects of green tea in poultry nutrition under various circumstances.

The aim of this study was to develop and examine a model of apoptosis and necrosis of hepatocytes induced by a damaging factor - adriamycin, correlating time after its administration with cell death type, and to investigate the localisation within the liver acinus of hepatocytes dying in these two ways. The results obtained in the present and previous studies were compared in order to make a map of cell death localisation in the liver acinus, showing the effect of time in action and dose of adriamycin. The experiment was performed on 32 female Wistar rats, divided into four groups: I and II - experimental, and III and IV - control. Adriamycin (3 mg/kg b.w.) was administered intraperitoneally to rats in groups I and II, and the rats were decapitated after four (group I) and eight (group II) weeks. Animals in control groups III and IV were given 0.5 mL of 0.9% NaCl solution, and decapitated after four and eight weeks respectively. Sections of the liver were examined with a three-stage immunohistochemical method. This method allowed to examine hepatocytes qualitatively and quantitatively for the presence of proteins involved in three types of apoptosis: induced by the mitochondrial pathway (caspase 3, 9), the intrinsic pathway related to endoplasmic reticulum stress (caspase 3, 12), and the extrinsic pathway (caspase 3, 8). One of the inflammatory markers, caspase 1, was also examined. The zonal localisation of all three types of apoptosis was assessed in the liver tissue. More oxidated hepatocytes indicated only signs of the internal mitochondrial pathway, whereas less oxidated hepatocytes induced the internal reticular pathway and the external apoptotic pathway. The period between adriamycin administration and hepatic cell investigation was a main factor of the process. A longer period post insult resulted in a more pronounced effect of the activation of apoptosis. Sections explored eight weeks after treatment with different doses of the drug (3 and 5 mg/kg in the previous study) showed a similar intensity of apoptosis.

The aim of this study was to develop and examine a model of apoptosis and necrosis of hepatocytes induced by a damaging factor - adriamycin, correlating time after its administration with cell death type, and to investigate the localisation within the liver acinus of hepatocytes dying in these two ways. The results obtained in the present and previous studies were compared in order to make a map of cell death localisation in the liver acinus, showing the effect of time in action and dose of adriamycin. The experiment was performed on 32 female Wistar rats, divided into four groups: I and II - experimental, and III and IV - control. Adriamycin (3 mg/kg b.w.) was administered intraperitoneally to rats in groups I and II, and the rats were decapitated after four (group I) and eight (group II) weeks. Animals in control groups III and IV were given 0.5 mL of 0.9% NaCl solution, and decapitated after four and eight weeks respectively. Sections of the liver were examined with a three-stage immunohistochemical method. This method allowed to examine hepatocytes qualitatively and quantitatively for the presence of proteins involved in three types of apoptosis: induced by the mitochondrial pathway (caspase 3, 9), the intrinsic pathway related to endoplasmic reticulum stress (caspase 3, 12), and the extrinsic pathway (caspase 3, 8). One of the inflammatory markers, caspase 1, was also examined. The zonal localisation of all three types of apoptosis was assessed in the liver tissue. More oxidated hepatocytes indicated only signs of the internal mitochondrial pathway, whereas less oxidated hepatocytes induced the internal reticular pathway and the external apoptotic pathway. The period between adriamycin administration and hepatic cell investigation was a main factor of the process. A longer period post insult resulted in a more pronounced effect of the activation of apoptosis. Sections explored eight weeks after treatment with different doses of the drug (3 and 5 mg/kg in the previous study) showed a similar intensity of apoptosis.

The aim of this study was to develop and examine a model of apoptosis and necrosis of hepatocytes induced by a damaging factor - adriamycin, correlating time after its administration with cell death type, and to investigate the localisation within the liver acinus of hepatocytes dying in these two ways. The results obtained in the present and previous studies were compared in order to make a map of cell death localisation in the liver acinus, showing the effect of time in action and dose of adriamycin. The experiment was performed on 32 female Wistar rats, divided into four groups: I and II - experimental, and III and IV - control. Adriamycin (3 mg/kg b.w.) was administered intraperitoneally to rats in groups I and II, and the rats were decapitated after four (group I) and eight (group II) weeks. Animals in control groups III and IV were given 0.5 mL of 0.9% NaCl solution, and decapitated after four and eight weeks respectively. Sections of the liver were examined with a three-stage immunohistochemical method. This method allowed to examine hepatocytes qualitatively and quantitatively for the presence of proteins involved in three types of apoptosis: induced by the mitochondrial pathway (caspase 3, 9), the intrinsic pathway related to endoplasmic reticulum stress (caspase 3, 12), and the extrinsic pathway (caspase 3, 8). One of the inflammatory markers, caspase 1, was also examined. The zonal localisation of all three types of apoptosis was assessed in the liver tissue. More oxidated hepatocytes indicated only signs of the internal mitochondrial pathway, whereas less oxidated hepatocytes induced the internal reticular pathway and the external apoptotic pathway. The period between adriamycin administration and hepatic cell investigation was a main factor of the process. A longer period post insult resulted in a more pronounced effect of the activation of apoptosis. Sections explored eight weeks after treatment with different doses of the drug (3 and 5 mg/kg in the previous study) showed a similar intensity of apoptosis.