The synthesis of (C16H14N3O2) ligand and (Cu2C32H28N6O12S2) complex are prepared in this work . The newly prepared compounds were characterized by IR spectroscopy and elemental analysis . The determination of the antimicrobial activity of ligand and its complex was carried out on sample of Escherichia coli , Klebsiella pneumonia , Staphylococcus aureus and Bacillus cereus . The quantitative antimicrobial activity test results proved that all the prepared compounds are very active against Bacillus cereus and that complex very active against Escherichia coli.. Quantum mechanical calculation confirmed the assumption of stability of prepared compounds which agreement with antimicrobial activity results .

ABSTRACT Escherichia coli was isolated from 56 dog fecal sample (69.14%) out of 81 collected samples. The antibacterial activity of E. coli isolate was primarily and secondarily screened on sold and liquid media. The results of primary screening showed that 56 isolates had antibacterial activity against tested gram positive and negative standard strains. By secondary screening E. coli isolate was able to produce antimicrobial agent in fermentation media at different incubation conditions, but larger amount of produced antimicrobial agent was obtained at 37Co, 24h, 180rpm by used seed and fermentation media. Different methods were used to determine the purity and to characterize the produced antimicrobial agent physically, chemically and biologically. The results of purity test revealed that the produced antimicrobial agent appeared as pure compound with only one spot in paper chromatography. The result of physical, chemical and biological characterization showed that the produced antimicrobial agent was peptide material with low toxicity 2350mgkg body weight.

In this study 15 rabbits were divided into three groups ,each group contain five rabbit were submitted to surgical section in the skin layer 3-5 cm long after anasethesia and cleaning the part from the hair by sheaving . The surgical section was made by scable and then the wound sutured by silk band 2.5 cm and then administrated the dexamethasone 1 mg / Kg body weight of animals of second and third grougs of this study . After that the skin spicemen were taken for histopathological sections to preperd slid. The first group was considered as control with no administrated of dexamthasone , the second group treated as 1 mg / Kg body weight dexamthasone and sections were taken after 7th days , the third group were also treated with dexamthasone at the 1 mg /Kg and the skin sample were taken after 14 th days. The present study it was found that dexamthasone have no effect on the wound healing after 7th days or 14th days but the collagenization , epithelization and fibroblast content were less in the dexamthasone group compered with control group . The vasicularity and the degree of inflammatory cells were more intense in the dexamthasone group compared with control group.

This study has been done to evaluate the effect of transverse and oblique anastomosis on esophageal healing. Partial esophagectomy were performed on sixteen adult dogs, animals were divided into two equal groups ,in the group one a transverse anastomosis ,while in group two oblique anastomosis were done . Two layers of simple interrupted pattern were used to closed the anastomosis site, the first layer was oppose the mucosa and submucosa by simple interrupted suture with the knots tided inside the lumen and the second layer was appose muscularis and adventitia by simple interrupted suture using catgut (2.0). The results of histopathological and radiological were revealed that ,the healing of group two was faster than group one ,which manifested by collagen fiber formation with complete mucosal formation in a shorter time compared with group one . In addition to that the mean degree of stenosis was less in second group at 30 days post operation(14.24±0.21), in spite of no significance difference between two groups at the level of p≤ 0.05.

This study was carried out juveniles common carp, Cyprinus carpio to determined the effect of (WSF) of crude oil on blood glucose and hemoglobin of blood values and glycogen of liver and muscles exposed to sub lethal effects for using different concentration. Study showed lower levels of blood hemoglobin and glycogen of liver and muscles, while blood glucose values have risen when exposed to concentrations of crude oil used.

The purpose of this study was to determinate The location, arrangement , the total number of fungiform papillae and number of those papillae in different regions of Twenty Tongues of Iraqi sheep (males) in different ages(two, four month embryo ,five month old and two year old) .Histological sections of fungiform papillae in different region from tongues were studied. Bapillae diameters and number of taste buds was assigned for each sample. The results showed that the papillae arise during embryological stage was thickened epithelium forming protrusion on the tongue surface. No significant differences was observed in the total number of the fungiform papillae in all ages. All papillae were distributed in the rostral part of the tongue, The papillae diameter showed significant increase respect to ages in all region. The taste buds were showed significant difference in number between 4 month embryo and 5 month old, while no significant between 5 month old and 2 year old. The number of taste buds for each papillae was more in cranial and caudal part compared with the middle part. The diameter of taste buds showed difference according to ages in all regions.

Liver and lung samples (25 for each) were collected from slaughtered cows and examend bacteriologicaly for the presence of Escherichia coli Eosin-Methylene Blue ( EMB ) and MacConkey agar positive culture (15 from liver and 12 from lung) were produced by cultivation of liver and lung samples Escherichia coli presence was confirmed biochemically in 53.3% of liver isolates and in 33.3% of lung isolates. Enterohemolytic activity was detected in 75%of both liver and lung isolates

ABSTRACT The aim of this study was to determine the minimum active dilution of two standardized type, representative honeys from two bees farm for 7 clinical isolates of coagulates-negative staphylococci. An agar incorporation technique was used to determine the minimum active dilution, with dilution steps for 1% (v/v) honey and 5% (v/v) for sugar syrup .The plates were inoculated with 10 µL spots of cultures of the isolates. the pasture honey inhibited at mean SD3.2± 0.7% (v/v),3.3 ± 0.5% (v/v) for the Manuk honey and 30.9 ± 1.9% (v/v) for the sugar syrup. Typical honeys are about eight times more potent against coagulase-negative staphylococci than bacterial inhibition were due to their osmolarity alone, but also the activity of honey by having its content

Open wounds have lost the barrier that protects tissues from bacterial invasion and allowfor the escape of vital fluids. The purpose of this study is to evaluate the enhancing oftherapeutic effect of Low Level Laser (LLL) and chitosan on the acceleration of openwound healing. Forty two adult rabbits were used. They divided into three equal groups (I,II and III). Wound was produced at the dorsal region by remove all thickness of skin atwidth of 3.5cm and of length 4 cm. Group I was left without treatment, while chitosanpowder and laser therapy were used in group II and III respectively. Results of two groupswere compared with control group. Histpathologicl results at the period of 3, 7, 14, 21 and28 days post operation reflected the presence of large number of fibroblast with formationof new blood vessels, also the collagen fiber become dense and regular in 14 days postoperation in irradiated groups. In 21days there was formation of new and normal epidermalclosed the rupture area of III group.

The main objective of this study is to demonstrate the histopathological changes and the efficacy of ethambutol of treatment mice infected with mycobacterium tuberculosis. Thirty of white Swisrland mice is 6-8 weeks age, weighted 20-25gm were used they were randomly divided into 3 groups contain of 10 animals for each group. - The 1st group (infected animal group) were inoculate.ed with 0.1 ml of bacterial suspension contain 1x108 cfu/ml intraperitonialy. - The 2nd group (group of infected-treated animal) were also infected as the fist group but after 30 day of infection were treated with 0.1 ml of ethambutol was given orally for 1 30 days. - The 3rd group maintained as a control and were inoculated with 1 ml of sterial normal saline intraperitonealy. At 60 day post infection all animals were sacrified and samples from different organs (liver, lungs, kidncys, spleen, intestine) were isolated for that histopathological examination. The result showed sever pathological lesion such as granulomatous lesions in lungs and livers of infected animals, with depletion of white pulp of spleen and conjestion with degenerative changes seen in kidneys and intestine with infiltration of inflammatory cells. While the infected-treated animals show mild or no pathological lesion in their internal organs.