peer reviewed | Objective: The present study was conducted to estimate the seroprevalence and associated risk factors of BoHV-1 in a dairy herd in the northeast of Algiers, Algeria. Materials and methods: The target area is in northeast of Algiers with humid to semi-dry climate and known for its economically important production of cattle. A total of 1066 randomly selected individual blood samples of dairy herd collected at 120 dairy farms from rural districts of northeast of Algiers were evaluated with antibodies against BoHV-1 using commercial ELISA kits, to determine the BoHV-1 infection status of the herds. A questionnaire submitted to the farmers during collection of the blood samples was used to collect data on potential BoHV-1 associated risk factors. Results: In the present study, the estimated farm and individual animal BoHV-1 seroprevalence levels were 58.33% and 14.16%, respectively. A logistic regression analysis of random-effects model revealed that the significant associated risk factors for the present farm and individual animal seroprevalence levels were rural district, cattle introduced to the farm, region, and hygiene. Conclusion: This study found higher seroprevalence of BoHV-1 in the northeast of Algiers. The results could be used in designing the prevention and control strategy of BoHV-1 in the northeastern part of Algeria.

Objective: The present study was conducted to estimate the seroprevalence and associated risk factors of bovine herpesvirus 1 (BoHV-1) in a dairy herd in the northeast of Algiers, Algeria. Materials and methods: The target area is in the northeast of Algiers with humid to semi-dry climate and known for its economically important production of cattle. A total of 1,066 randomly selected individual blood samples of dairy herd collected at 120 dairy farms from rural districts of northeast of Algiers were evaluated with antibodies against BoHV-1 using commercial enzyme-linked immunosorbent assay kits, to determine the BoHV-1 infection status of the herds. A questionnaire submitted to the farmers during collection of the blood samples was used to collect data on potential BoHV-1 associated risk factors. Results: In the present study, the estimated farm and individual animal BoHV-1 seroprevalence levels were 58.33% and 14.16%, respectively. A logistic regression analysis of the random-effects model revealed that the significant associated risk factors for the present farm and individual animal seroprevalence levels were rural district, cattle introduced to the farm, region, and hygiene. Conclusion: This study found higher seroprevalence of BoHV-1 in the northeast of Algiers. The results could be used in designing the prevention and control strategy of BoHV-1 in the northeastern part of Algeria. [J Adv Vet Anim Res 2019; 6(1.000): 60-65]

Objective: The present study was conducted to estimate the seroprevalence and associated risk factors of bovine herpesvirus 1 (BoHV-1) in a dairy herd in the northeast of Algiers, Algeria. Materials and methods: The target area is in the northeast of Algiers with humid to semi-dry climate and known for its economically important production of cattle. A total of 1,066 randomly selected individual blood samples of dairy herd collected at 120 dairy farms from rural districts of northeast of Algiers were evaluated with antibodies against BoHV-1 using commercial enzyme-linked immunosorbent assay kits, to determine the BoHV-1 infection status of the herds. A ques­tionnaire submitted to the farmers during collection of the blood samples was used to collect data on potential BoHV-1 associated risk factors. Results: In the present study, the estimated farm and individual animal BoHV-1 seroprevalence levels were 58.33% and 14.16%, respectively. A logistic regression analysis of the random-effects model revealed that the significant associated risk factors for the present farm and individual ani­mal seroprevalence levels were rural district, cattle introduced to the farm, region, and hygiene. Conclusion: This study found higher seroprevalence of BoHV-1 in the northeast of Algiers. The results could be used in designing the prevention and control strategy of BoHV-1 in the northeast­ern part of Algeria. J. Adv. Vet. Anim. Res., 6(1): 60-65, March 2019

A febre maculosa brasileira é uma doença grave e letal para seres humanos causada pela bactéria Rickettsia rickettsii. No estado de São Paulo, SP, Brasil, o agente etiológico desta enfermidade é transmitido pelo carrapato Amblyomma sculptum. Conforme descrito na literatura científica, os cavalos infectados com esta bactéria produzem uma forte resposta imune e podem ser importantes sentinelas para a detecção da doença. A presente investigação realizou um levantamento sorológico em cavalos de cinco fazendas do Vale do Paraíba, São Paulo, Brasil, à procura de anticorpos contra Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali e Rickettsia bellii. Em cada fazenda, também foram coletados carrapatos identificados taxonomicamente e examinados por PCR em tempo real para o DNA de Rickettsia spp. Foram coletadas amostras de sangue de 206 cavalos e coletados 334 carrapatos desses animais entre os meses de janeiro e dezembro de 2017. Oitenta carrapatos foram identificados como A. sculptum e 254 Dermacentor nitens. Das amostras de sangue, 7,3% soroconverteram para Rickettsia spp., sendo que, 0,97% apresentaram soropositividade homóloga para R. bellii. Nenhum dos 80 carrapatos de A. sculptum foi positivo com o emprego de PCR em tempo real para Rickettsia spp. Embora não tenham sido detectados carrapatos infectados por Rickettsia spp em cinco fazendas do Vale do Paraíba, os animais apresentaram reações sorológicas positivas para este agente. Assim, outros estudos abrangentes deverão ser realizados na área investigando hospedeiros e vetores, gerando dados para medidas de controle da transmissão da febre maculosa brasileira. | Brazilian spotted fever is a serious and lethal illness for humans and is caused by the Rickettsia rickettsii bacteria. In the state of São Paulo/SP (Brazil), the etiological agent of this disease is transmitted by the Amblyomma sculptum tick. It was already shown that horses infected with this bacteria produce a strong immune response and could be important sentinels for the detection of the disease in a proper region. The present investigation performed a serological survey in horses from five farms of Vale do Paraíba, São Paulo state, Brazil, searching for antibodies against, Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali, and Rickettsia bellii. In each farm, ticks were also collected that were taxonomically identified and examined by real-time PCR for Rickettsia spp DNA. Blood samples were collected from 206 horses, and 334 ticks were picked up from these animals from January to December 2017. Eighty ticks wereA. sculptum and 254 Dermacentor nitens. Of the blood samples, 7.3% seroconverted to Rickettsia spp. Of these, 0.97% had a positive serological response to R. bellii. None of the 80 A. sculptum ticks were positive through real-time PCR for Rickettsia spp. Although there was no detection of ticks infected by Rickettsia spp in five farms of Paraíba Valley, the horses presented serological positive reactions against this agent. Thus, further large studies should be conducted in the area targeting hosts and vectors to generate data for control measures of the transmission of Brazilian spotted fever. 

Brazilian spotted fever is a serious and lethal illness for humans and is caused by the Rickettsia rickettsii bacteria. In the state of São Paulo/SP (Brazil), the etiological agent of this disease is transmitted by the Amblyomma sculptum tick. It was already shown that horses infected with this bacteria produce a strong immune response and could be important sentinels for the detection of the disease in a proper region. The present investigation performed a serological survey in horses from five farms of Vale do Paraíba, São Paulo state, Brazil, searching for antibodies against, Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali, and Rickettsia bellii. In each farm, ticks were also collected that were taxonomically identified and examined by real-time PCR for Rickettsia spp DNA. Blood samples were collected from 206 horses, and 334 ticks were picked up from these animals from January to December 2017. Eighty ticks were A. sculptum and 254 Dermacentor nitens. Of the blood samples, 7.3% seroconverted to Rickettsia spp. Of these, 0.97% had a positive serological response to R. bellii. None of the 80 A. sculptum ticks were positive through real-time PCR for Rickettsia spp. Although there was no detection of ticks infected by Rickettsia spp in five farms of Paraíba Valley, the horses presented serological positive reactions against this agent. Thus, further large studies should be conducted in the area targeting hosts and vectors to generate data for control measures of the transmission of Brazilian spotted fever.

Foi investigada a existência de diferença na deposição de gordura e no metabolismo lipídico em cavalos de duas raças distintas, com aptidões diferentes, porém, utilizadas para o mesmo esporte. Foram avaliados 20 cavalos Puro Sangue Árabe e 20 cavalos Puro Sangue Ingleses treinados para corrida. As avaliações foram escore de condição corporal, peso e colheita de sangue para determinação de triglicerídeos, colesterol total e ácidos graxos não esterificados. Foi realizada a ultrassonografia da espessura de camada de gordura subcutânea sobre o músculo Longissimus dorsi entre a 17º e 18º costela, a espessura de camada de gordura subcutânea sobre o músculo glúteo médio utilizando o acetábulo como referência e o corte transversal do mesmo músculo. Os cavalos Árabes de corrida apresentaram maior deposição de gordura na camada subcutânea dos músculos glúteo médio e Longissimus dorsei que os cavalos Puro Sangue Ingleses. Tais fatosindicam uma diferença racial que o treinamento, ainda que semelhante a todos, não foi capaz de igualar. | This study investigated the existence of difference of fat deposition and lipid metabolism in horses with different races and skills that were used for the same kind of sport. 20 Purebred Arabian and 20 Thoroughbred horses trained for flat race were evaluated. The analyses performed were body condition score, weight and blood collected for determination of triglycerides, total cholesterol and non-esterified fatty acids. Ultrasonography of the thickness of the subcutaneous fat layer was performed on the Longissimus dorsi muscle between the 17th and 18th rib, the thickness of the subcutaneous fat layer on the Gluteus medius muscle using the acetabulum as reference, and the cross section of the same muscle. Race-trained Arabian horses showed greater fat layer deposition in the Gluteus medius and Longissimus dorsei musclesthan Thoroughbred horses. These facts indicate that there is a metabolic difference, besides the phenotype, between the races. They also indicate the need to study specific physical conditioning programs for each kind of race.

This study investigated the existence of difference of fat deposition and lipid metabolism in horses with different races and skills that were used for the same kind of sport. 20 Purebred Arabian and 20 Thoroughbred horses trained for flat race were evaluated. The analyses performed were body condition score, weight and blood collected for determination of triglycerides, total cholesterol and non-esterified fatty acids. Ultrasonography of the thickness of the subcutaneous fat layer was performed on the Longissimus dorsi muscle between the 17th and 18th rib, the thickness of the subcutaneous fat layer on the Gluteus medius muscle using the acetabulum as reference, and the cross section of the same muscle. Race-trained Arabian horses showed greater fat layer deposition in the Gluteus medius and Longissimus dorsei muscles than Thoroughbred horses. These facts indicate that there is a metabolic difference, besides the phenotype, between the races. They also indicate the need to study specific physical conditioning programs for each kind of race.

A concordância entre métodos de avaliação subjetiva e objetiva foi estudada para identificar claudicação nos membros pélvicos antes e após a realização dos testes de flexão. Vinte e nove cavalos foram equipados com um sistema de sensores inerciais sem fio e avaliados durante sete momentos enquanto troteavam. Vídeos foram gravados para serem avaliados por três veterinários com diferentes níveis de experiência, a fim de avaliar a concordância entre os dois métodos diferentes e entre os avaliadores. Os avaliadores e a avaliação objetiva tiveram uma baixa taxa de concordância, com exceção de uma moderada concordância entre avaliação objetiva e avaliador 1 para identificar claudicação após o teste de flexão total esquerdo e uma moderada concordância na avaliação da resposta aos testes, entre avaliação objetiva e avaliador 2, apóso teste de flexão distal direito. Isso demonstra que houve uma baixa concordância entre os avaliadores e entre estes e a avaliação objetiva para identificação da claudicação, mensuração do grau de claudicação e resposta aos testes de flexão. | The agreement between subjective and objective evaluation methods was studied to identify claudication in the pelvic limbs of horses before and after flexion tests were performed. Twenty-nine horses were equipped with a wireless system of inertial sensors and evaluated during seven times while trotting. Videos were recorded to be evaluated by three veterinarians, with different levels of experience, to evaluate the agreement between the two different methods and between the evaluators. The evaluators and the objective evaluation had a low rate of agreement, with the exception of moderate agreement between the objective evaluation and evaluator 1 to identify lameness after the left total flexion and moderate agreement in evaluating the response to the tests, between objective evaluation and evaluator 2, after rightdistal flexion. This shows that there was a low agreement among the evaluators and between them and the objective evaluation for identifying lameness, measuring the degree of lameness and the response to the flexion tests.

The agreement between subjective and objective evaluation methods was studied to identify claudication in the pelvic limbs of horses before and after flexion tests were performed. Twenty-nine horses were equipped with a wireless system of inertial sensors and evaluated during seven times while trotting. Videos were recorded to be evaluated by three veterinarians, with different levels of experience, to evaluate the agreement between the two different methods and between the evaluators. The evaluators and the objective evaluation had a low rate of agreement, with the exception of moderate agreement between the objective evaluation and evaluator 1 to identify lameness after the left total flexion and moderate agreement in evaluating the response to the tests, between objective evaluation and evaluator 2, after right distal flexion. This shows that there was a low agreement among the evaluators and between them and the objective evaluation for identifying lameness, measuring the degree of lameness and the response to the flexion tests.

Na região da represa de Guarapiranga localizada na área metropolitana de São Paulo, têm sido relatados casos humanos de Febre Maculosa Brasileira (FMB), uma doença transmitida por carrapatos causada pela bactéria Rickettsia rickettsii. Nesta área de estudo, R. rickettsii é conhecida por ser transmitida aos seres humanos pelo Amblyomma aureolatum, um carrapato de cão que não está associado a cavalos. Em outras áreas endêmicas da FMB, a transmissão de R. rickettsii está associada ao Amblyomma sculptum, uma espécie de carrapato que normalmente infesta capivaras e cavalos. A represa de Guarapiranga possui populações abundantes de capivaras e cavalos; no entanto, como nada se sabe sobre um possível ciclo de transmissão de R. rickettsii por A. sculptum nessa área, este estudo avaliou essa transmissão realizando um levantamento sorológico em cavalos que vivem na região da represa de Guarapiranga. Um total de 206 equídeos que vivem nas margens da represa de Guarapiranga foram testados sorologicamente para cinco espécies de Rickettsia, sendo quatro do grupo da FMB (R. rickettsii, R. parkeri, R. amblyommatis, R. rhipicephali) e um do grupo basal (R. bellii). No geral, 171 (83%) equídeos reagiram positivamente a pelo menos uma espécie de Rickettsia. Um total de 160 (78%), 123 (60%), 80 (39%), 72 (35%) e 71 (34%), reagiram a R. bellii, R. rickettsii, R. parkeri, R. rhipicephali e R. amblyommatis, respectivamente, com títulos finais variando de 64 a 1024 para R. bellii e 64 a 512 para as quatro espécies restantes de Rickettsia. Os títulos finais para R. bellii (mediana: 256) foram significativamente maiores (P <0,05) do que os títulos para as outras quatro espécies de Rickettsia, para os quais os valores medianos variaram de 64 a 128. Um total de 65 (32%) equideos, os soros mostraram títulos finais para R. bellii pelo menos quatro vezes maior que os de qualquer um dos outros quatro antígenos, indicando que eles foram expostos a R. bellii ou a uma espécie muito próxima. Os resultados obtidos fornecem evidências sorológicas de que os equídeos amostrados não eram frequentemente expostos a carrapatos infectados por R. rickettsii. Como os cavalos são um sentinela altamente adequado para a transmissão de R. rickettsii por A. sculptum, a conclusão obtida foi que essa espécie de carrapato não tem papel epidemiológico na transmissão da bactéria na área endêmica de FMB da represa de Guarapiranga na região metropolitana de São Paulo. | The Guarapiranga Dam region, in the metropolitan area of São Paulo, has been an endemic area for Brazilian spotted fever (BSF), a tick-borne disease caused by the bacterium Rickettsia rickettsii. In this particular area, R. rickettsii is known to be transmitted to humans by Amblyomma aureolatum, a typical dog tick that is not associated with horses. In other BSF-endemic areas, R. rickettsii transmission is associated with Amblyomma sculptum, a tick species that typically infest capybaras and horses. The Guarapiranga Dam bears abundant populations of capybaras and horses; however, since nothing is known about a possible cycle of transmission of R. rickettsii by A. sculptum in this area, this study evaluated such transmission by performing a serosurvey of horses living in the Guarapiranga Dam region. A total of 206 equids living in the margins of the Guarapiranga Dam were serologically tested for antibodies reactive to five Rickettsia species, four of the spotted fever group (R. rickettsii, R. parkeri, R. amblyommatis, R. rhipicephali) and one basal group species, R. bellii. Overall, 171 (83%) equids reacted positively to at least one Rickettsia species. A total of 160 (78%), 123 (60%), 80 (39%), 72 (35%), and 71 (34%), equid sera reacted to R. bellii, R. rickettsii, R. parkeri, R. rhipicephali, and R. amblyommatis, respectively, with endpoint titers ranging from 64 to 1024 for R. bellii, and 64 to 512 for the remaining four Rickettsia species. Endpoint titers to R. bellii (median: 256) was significantly higher (P<0.05) than the endpoint titers to the other four Rickettsia species, for which the median values varied from 64 to 128. A total of 65 (32%) equid sera showed endpoint titers to R. bellii at least 4-fold higher than those to any of the other four antigens, indicating that they have been exposed to R. bellii or a very closely related species. Our results provide serological evidence that the sampled equids were not frequently exposed to R. rickettsii-infected ticks. Since horses are a highly suitable sentinel for R. rickettsii transmission by A. sculptum, we conclude that this tick species has no epidemiological role in the transmission of R. rickettsii in the BSF-endemic area of the Guarapiranga Dam in the metropolitan area of São Paulo Municipality.

The Guarapiranga Dam region, in the metropolitan area of São Paulo, has been an endemic area for Brazilian spotted fever (BSF), a tick-borne disease caused by the bacterium Rickettsia rickettsii. In this particular area, R. rickettsii is known to be transmitted to humans by Amblyomma aureolatum, a typical dog tick that is not associated with horses. In other BSF-endemic areas, R. rickettsii transmission is associated with Amblyomma sculptum, a tick species that typically infest capybaras and horses. The Guarapiranga Dam bears abundant populations of capybaras and horses; however, since nothing is known about a possible cycle of transmission of R. rickettsii by A. sculptum in this area, this study evaluated such transmission by performing a serosurvey of horses living in the Guarapiranga Dam region. A total of 206 equids living in the margins of the Guarapiranga Dam were serologically tested for antibodies reactive to five Rickettsia species, four of the spotted fever group (R. rickettsii, R. parkeri, R. amblyommatis, R. rhipicephali) and one basal group species, R. bellii. Overall, 171 (83%) equids reacted positively to at least one Rickettsia species. A total of 160 (78%), 123 (60%), 80 (39%), 72 (35%), and 71 (34%), equid sera reacted to R. bellii, R. rickettsii, R. parkeri, R. rhipicephali, and R. amblyommatis, respectively, with endpoint titers ranging from 64 to 1024 for R. bellii, and 64 to 512 for the remaining four Rickettsia species. Endpoint titers to R. bellii (median: 256) was significantly higher (P<0.05) than the endpoint titers to the other four Rickettsia species, for which the median values varied from 64 to 128. A total of 65 (32%) equid sera showed endpoint titers to R. bellii at least 4-fold higher than those to any of the other four antigens, indicating that they have been exposed to R. bellii or a very closely related species. Our results provide serological evidence that the sampled equids were not frequently exposed to R. rickettsii-infected ticks. Since horses are a highly suitable sentinel for R. rickettsii transmission by A. sculptum, we conclude that this tick species has no epidemiological role in the transmission of R. rickettsii in the BSF-endemic area of the Guarapiranga Dam in the metropolitan area of São Paulo Municipality.

Pododermatite proliferativa crônica equina (cancro) é uma doença hipertrófica dos cascos, caracterizada por uma proliferação excessiva dos tecidos produtores de túbulos, o que resulta de uma disqueratose dos queratinócitos. Concentra-se principalmente na região da ranilha e seus sulcos, mas algumas vezes pode afetar também talões, bulbos, sola e parede do casco. A etiologia ainda não foi confirmada. Agentes infecciosos como bactérias anaeróbias, vírus, espiroquetas e fungos já foram isolados de tecidos afetados, mas cada vez mais uma hipótese imune vem sido discutida, possivelmente relacionada a uma predisposição genética. Este relato traz o caso de um Brasileiro de Hipismo, 10 anos, afetado no membro pélvico direito há mais de dois anos sem um diagnóstico conciso. O êxito foi obtido com o tratamento consistindo em Prednisolona oral e Purple Mush® tópico. | Chronic equine proliferative pododermatitis (canker) is a chronic hypertrophic disease of the hoof, denoted by an excessive proliferation of the horn-producing tissues, which results from a dyskeratosis of the keratinocytes. It occurs mainly at the frog and sulci regions, but can sometimes also affect the heels, sole, bulbs or hoof wall. The etiology still remains unclear. Infectious agents such as anaerobic bacteria, viruses, spirochetes and fungi have been isolated from diseased tissues, but, moreover, an immune cause has been suggested, possibly related to a genetic predisposition. This report shows a case of a Brazilian Sport Horse, 10 years old, that had been affected on the right hindlimb for more than two years without a concise diagnosis. Success was achieved with the treatment consisting of oral prednisolone and topical Purple Mush®.

Chronic equine proliferative pododermatitis (canker) is a chronic hypertrophic disease of the hoof, denoted by an excessive proliferation of the horn-producing tissues, which results from a dyskeratosis of the keratinocytes. It occurs mainly at the frog and sulci regions, but can sometimes also affect the heels, sole, bulbs or hoof wall. The etiology still remains unclear. Infectious agents such as anaerobic bacteria, viruses, spirochetes and fungi have been isolated from diseased tissues, but, moreover, an immune cause has been suggested, possibly related to a genetic predisposition. This report shows a case of a Brazilian Sport Horse, 10 years old, that had been affected on the right hindlimb for more than two years without a concise diagnosis. Success was achieved with the treatment consisting of oral prednisolone and topical Purple Mush®.

Objective: House musk shrew (Suncus murinus), a small experimental animal with low body fat, may be a possible model for human lipodystrophy. Leptin is an adipocyte-derived hormone thought to have an important role in the pathophysiology of lipodystrophy. The objectives of this study were to clarify the structure and distribution of suncus leptin. Materials and methods: To determine the primary structure of suncus leptin, we cloned the suncus Lep cDNA using the rapid amplification of cDNA ends method. The obtained amino acid (aa) sequence was compared with other mammals and the protein structure prediction was performed. Results: The suncus Lep cDNA encodes 170 aa. The putative suncus leptin precursor has a pre­dicted signal peptide of 21 aa, and the mature leptin comprises 149 aa. The mature leptin is 75%–82% homologous to that of other species. Insertion of the three aa, VPQ, not seen in other mammals was found. This VPQ insertion is thought to be due to a nucleotide insertion of nine bases by slippage-like microindels. The predicted 3D structure of suncus leptin exhibited a typi­cal four a-helix structure, however, the VPQ region protruded compared with human leptin. Lep mRNA expression was observed only in white and brown adipose tissues. Conclusion: This study revealed the structure and distribution of suncus leptin. Because the addi­tion of VPQ, which is not found in other mammals, was observed, suncus leptin attracts attention to its physiological action, and to the possibility of being a model of human lipodystrophy. J. Adv. Vet. Anim. Res., 6(1): 1-8, March 2019

Objective: House musk shrew (Suncus murinus), a small experimental animal with low body fat, may be a possible model for human lipodystrophy. Leptin is an adipocyte-derived hormone thought to have an important role in the pathophysiology of lipodystrophy. The objectives of this study were to clarify the structure and distribution of suncus leptin. Materials and methods: To determine the primary structure of suncus leptin, we cloned the suncus Lep cDNA using the rapid amplification of cDNA ends method. The obtained amino acid (aa) sequence was compared with other mammals and the protein structure prediction was performed. Results: The suncus Lep cDNA encodes 170 aa. The putative suncus leptin precursor has a predicted signal peptide of 21 aa, and the mature leptin comprises 149 aa. The mature leptin is 75%82% homologous to that of other species. Insertion of the three aa, VPQ, not seen in other mammals was found. This VPQ insertion is thought to be due to a nucleotide insertion of nine bases by slippage-like microindels. The predicted 3D structure of suncus leptin exhibited a typical four a-helix structure, however, the VPQ region protruded compared with human leptin. LepmRNA expression was observed only in white and brown adipose tissues. Conclusion: This study revealed the structure and distribution of suncus leptin. Because the addition of VPQ, which is not found in other mammals, was observed, suncus leptin attracts attention to its physiological action, and to the possibility of being a model of human lipodystrophy. [J Adv Vet Anim Res 2019; 6(1.000): 1-8]

Objective: Investigating the antibiotic and antioxidant benefits of medicinal herbs to enrich the serum immune responses of chicken meat. Materials and Methods: A total of 1,080 Ross 308 broilers were reared up to 42 days. The broilers were divided randomly into nine assemblies, with each sectioned into three replicates. The first and second were supplemented with 0.25% and 0.50% of coriander seeds, respectively, while the third and fourth with 0.25% and 0.50% of rosemary leaves, respectively. A mixture of herbs from the two plants were added to fifth, sixth, seventh, and eighth treatments [(0.50% coriander seeds + 0.50% rosemary leaves), (0.25% coriander seeds + 0.50% rosemary leaves), (0.50% coriander seeds + 0.25% rosemary leaves), and (0.25% coriander seeds + 0.25% rosemary leaves)], respec­tively, whereas chicks in the ninth as a control group. Results: The results showed the pH for the thigh and breast of the carcass were measured. Glycogen levels, serum immunity (H, L, Hlration, Albumin, Globulin, and A/G ratio at 28 days and alanine aminotransferase, aspartate aminotransferase, and cholesterol at 42 days). The mineral deposits in the chicken meat were measured for Mg, Fe, Ca, Na, J, and total N. The fifth treatment had a significantly higher glycogen ratio (p &lt; 0.05). pH measurements for the thigh and breast were done immediately, 4, 12, and 24 h after slaughter. For the thigh, the seventh treatment was highest immediately and at 12 h. For the breast, significant differences were only noted at 12 h for chickens on a coriander diet. Conclusion: It is concluded that these additives have a positive effect on some of the blood pro­files, carcass characteristics, and mineral composition of chicken meat. J. Adv. Vet. Anim. Res., 6(1): 33-43, March 2019

Objective: Investigating the antibiotic and antioxidant benefits of medicinal herbs to enrich the serum immune responses of chicken meat. Materials and Methods: A total of 1,080 Ross 308 broilers were reared up to 42 days. The broilers were divided randomly into nine assemblies, with each sectioned into three replicates. The first and second were supplemented with 0.25% and 0.50% of coriander seeds, respectively, while the third and fourth with 0.25% and 0.50% of rosemary leaves, respectively. A mixture of herbs from the two plants were added to fifth, sixth, seventh, and eighth treatments [(0.50% coriander seeds + 0.50% rosemary leaves), (0.25% coriander seeds + 0.50% rosemary leaves), (0.50% coriander seeds + 0.25% rosemary leaves), and (0.25% coriander seeds + 0.25% rosemary leaves)], respectively, whereas chicks in the ninth as a control group. Results: The results showed the pH for the thigh and breast of the carcass were measured. Glycogen levels, serum immunity (H, L, Hlration, Albumin, Globulin, and A/G ratio at 28 days and alanine aminotransferase, aspartate aminotransferase, and cholesterol at 42 days). The mineral deposits in the chicken meat were measured for Mg, Fe, Ca, Na, J, and total N. The fifth treatment had a significantly higher glycogen ratio (p &lt; 0.05). pH measurements for the thigh and breast were done immediately, 4, 12, and 24 h after slaughter. For the thigh, the seventh treatment was highest immediately and at 12 h. For the breast, significant differences were only noted at 12 h for chickens on a coriander diet. Conclusion: It is concluded that these additives have a positive effect on some of the blood pro-files, carcass characteristics, and mineral composition of chicken meat. [J Adv Vet Anim Res 2019; 6(1.000): 33-43]