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Correction note for article: Abdel-Maguid, Doaa Safwat et al. Fraudulence Risk Strategic Assessment of Processed Meat Products. Vol. 9, n. 3, p. 81-90, July 2019.
2019
Editor-in-Chief Prof. Mahmoud Rushdi
This to notify the corrections for article: Abdel-Maguid, Doaa Safwat et al. Fraudulence Risk Strategic Assessment of Processed Meat Products. Journal of Advanced Veterinary Research, Vol. 9, n. 3, p. 81-90, July 2019. This sentence was edited: Fifty samples from 6 different meat products (page:82).The following figures’ captions were edited:Fig. 12. Paraffin sections showing the adulteration of minced meat. a: A wall of the visceral organ. Note the thick layer of smooth muscle fibers. b, c: digitally colored scanned electron micrograph showing fungal contamination (blue colored).Fig. 13. Digitally colored scanned electron micrograph showing the adulteration of minced meat a: Meat sample contaminated by bacteria and yeast (brown colored) and adulterated by fibrous tissue (violet colored).This paragraph in the discussion was edited: None of the examined samples were adulterated with hollow organs, an observation that is not in agreement with the study by Abdel Hafeeze et al. (2016). Page 88This paragraph in the discussion was edited: The smooth muscle fibers of visceral organs were detected in the luncheon meat samples. The degenerative muscle fibers of the heart were detected in the kofta samples, these findings are in accordance with those of Inal (1992), who reported that the muscle cells of intestinal mucosa and heart muscles were detected in salami and sausage samples. Page 88. Editors apologize for typo mistake in during the reviewing and editing processes.
Show more [+] Less [-]The ameliorative effect of methanolic red carrot extract and vitamin E against cadmium-induced testicular toxicity in rats
2019
Kamel M. A. Hassanin | Ahmed Abdel-Wahab | Ahmed A Mahmoud | Abdel-Razik H. AbdelRazik | Walaa I. E. Abdel-Badeea
The current study aimed to investigate the effects of methanolic redcarrot extract (MRCE) against cadmium intoxication on testis of adult Wisteralbino male rats. For that purpose, forty eight Wister albino male rats wererandomly divided into four groups (12 rats per group). Group 1 (normalcontrol), animals received corn oil. Group 2 (cadmium group), animalsreceived cadmium chloride (CdCl2) at a dose of (5 mg/ kg BW). Group 3(cadmium &Vit. E group), animals received vitamin E at a dose of(400mg/kg BW) and CdCl2at a dose of (5 mg/kg BW). Group 4 (cadmium &red carrot extract, animals received methanol: water red carrot extract (1:1)at a dose of 400 mg/kg and CdCl2 at a dose of (5 mg/kg BW). All rats werereceived their corresponding treatment orally by gastric gavage daily for 4weeks. Result of organ weight analysis in Cd –exposed rats showed adecrease in testes weight. On the contrary, MRCE and vitamin Eprophylactic co-treatments with cadmium showed significant increase intestis weights in comparison to cadmium group (P<0.05). Moreover, spermconcentrations were reduced markedly with cadmium while they wereupturned greatly after prophylactic co-treatment with either MRCE orvitamin E (P<0.05). Moreover, cadmium induced a significant increase intesticular malondialdehyde (MDA) and significant decrease of totalantioxidant capacity (TAC) but both MRCE and vitamin E supplementationsucceeded markedly to produce a significant reduction in testicular MDAand noticeable increase of TAC level. Thus, MRCE and vitamin E could beconsidered optimal prophylactic treatments to protect the testis of rats fromcadmium intoxication.
Show more [+] Less [-]Microbiological evaluation of chicken meat products
2019
F. A. Khalafalla | Fatma, H. M. Ali | A. El-Fouley
The aim of the present study was to compare the microbiological quality and safety of chicken products collected from a poultry processing plant and from the retail market. The collected samples represented 120 chicken product samples (mortadella, frankfurters, burgers, nuggets, fillet and fajita); 60 samples were collected from a poultry processing plant and 60 samples were from retail markets. For assessing the microbiological quality of these products, total bacterial count (TBC), most probable number (MPN) of coliforms and total mold and yeasts were determined. While, for evaluating the safety of collected products, Staphylococcus aureus, Salmonella, E. coli and Listeria monocytogenes were investigated. As well as, sensory evaluation of collected products was carried out. It was found that the bacterial counts in samples collected from processing plants were lower than corresponding samples collected from retail market. For instance, the obtained mean values of TBC in processing plant samples were 1x10, 4x102, 2x10, 2x10, 3x10 and 6x10 CFU/g in case of chicken mortadella, chicken frank, chicken nuggets, chicken burger, chicken fillet and chicken fajita, respectively. While for retail market samples, TBC mean values were 2x10, 2x10, 3x10, 3x10, 4x10 and 3x10 CFU/g in chicken mortadella, chicken frank, chicken nuggets, chicken burger, chicken fillet and chicken fajita, respectively. It was evident that most of examined chicken product samples either from processing plant or retail markets were contaminated with investigated foodborne pathogens, namely; Staphylococcus aureus, Salmonella, E. coli and Listeria monocytogenes, in addition to contamination with mold and yeasts. In conclusion, the rate of contamination of chicken products from retail markets was higher than corresponding products obtained from processing plant, which is attributed to contamination of chicken products through bad handling during transportation, storage and marketing, as well as growth of contaminants as a result of improper storage conditions including temperature and humidity.
Show more [+] Less [-]A study on the effect of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams
2019
Ahmad S. Mostafa | Mostafa M. Farghal
The objective of this study was to detect the influence of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams. The study was carried out at agricultural research center belonging to Minia University, Shosha village, Samalut city, El-Minia. Ten crossbred and five adult Ossimi rams were used during this study. Every ram was allowed to breed two ewes to evaluate its sexual behaviour for a twenty minutes test. Blood samples were collected three times per season at two weeks interval from each ram during the period of experiment to measure testosterone concentrations. Also, scrotal circumference was measured at the same frequency and time interval as blood samples. Sexual behaviour was better in old rams than young ones (p<0.01), but it was not affected by breed or season (p>0.05). Serum testosterone level was higher at autumn (7.13±02.9 ng/ml) than at winter (5.54±0.29 ng/ml) in crossbred rams (p<0.01), while in Ossimi rams, higher level was obtained in spring (8.24±.39 ng/ml) than in summer (3.99±0.40 ng/ml). Mean scrotal circumference was higher in older rams (30.20±1.20 cm) than younger rams (22.53±1.27 cm). There was no significant difference for measures of scrotal circumference between crossbred rams (30.86±1.09 cm) and Ossimi rams (30.93±1.06 cm). Season had no significant effect on scrotal circumference in crossbred rams, whereas in Ossimi rams the effect of season was significantly obvious with the highest and lowest values were recorded in spring (31.0±1.05 cm) and winter (30.60±1.17 cm) respectively. It could be concluded from this study that the age of rams had a great influence on their sexual behaviour and scrotal circumference, while the effect of breed and season on sexual behaviour and scrotal circumference was not clear.
Show more [+] Less [-]Dirofilaria immitis infestation in imported police (K-9) dogs in Iraq:
2019
Karima Akool Al-Salihi | Ahmed Hameed Al-Dabhawi | Hayder M. Al-Rammahi | Fatima Atiya Kareem
Dirofilaria immitis, the cause of heartworm infestation (HWI) or dirofilariasis, affects members of the Canidae and remains a worldwide clinical problem. In Iraq, dirofilariasis was believed absent until 2009, when the Karbala Governorate was reported as an endemic area for canine dirofilariasis. Consequently, this study intended to investigate the occurrence of Dirofilaria immitis in police dogs in one police academy in Iraq and to study the gross and histopathological changes in 5 dead dogs, as well as to identify the species of the causative parasite using PCR technique. Thirty-nine police dogs, aged between 6 months and 12 years were included in this study. For the microfilariae investigation, 5 ml blood samples were collected from all dogs in EDTA tubes and examined by Knott’s method. The systemic necropsy performed in five dead dogs showed severe clinical signs of dirofilariasis and tissue specimens were sent for routine histopathological processing. For the molecular analysis, adult worms of the detected Dirofilaria spp. were used for DNA extraction andamplification of the cox1 gene. Fifteen of 39 (38.46%) dogs were diagnosed with moderate to severe microfilariasis. The dead dogs revealed typical severe clinical signs of dirofilariasis. Moreover, typical gross and histopathological changes were also seen, accompanied by generalized thromboembolic lesions, suggesting the occurrence of the caval syndrome. The PCR investigation confirmed that D. immitis was the species present in Iraq. In conclusion, this study establishes that Iraq is a newly reported endemic area for dirofilariasis. Moreover, the infestation occurring in these cases most probably happened inside Iraq. The authors recommend doing further epidemiological studies concerning the occurrence of D. immitis in local dogs as well as in the imported dogs in all Iraqi governorates to better understand the epidemiological map of this disease and to introduce an active treatment and preventive program. Awareness and education regarding this disease should be provided to the veterinarians, dog guiders and people in direct contact with dogs, as this disease is one of the important zoonotic diseases.
Show more [+] Less [-]Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
2019
Jackeliny Santos Costa | Andréia Lima Tome Melo | Rute Witter | Thabata Anjos Pacheco | Cristiane Silva Chitarra | Izabelle Thayná Soares Carvalho | Luciano Nakazato | Valeria Dutra | Richard Campos Pacheco | Daniel Moura de Aguiar
The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.
Show more [+] Less [-]Efficiency of motivational tools and social media use in the control of canine obesity
2019
Bruna Rodrigues Stafoche | Márcia Marques Jericó
The increasing number of obese dogs in veterinary practice has been a warning to veterinarians about the need to develop new tools to approach the obese patient, aiming to attain satisfactory results in weight loss programs. A total of 18 obese or overweight dogs with no evidence of endocrine disorders were selected for this study. These animals were randomly divided into two groups with 9 animals each. In one of the groups, strategic tools were used by their tutors, aimed at behavioral and environmental modification, and this group was assisted daily through communication using a digital instant messaging app (WhatsApp(R)). In the other group, these tools were not used, and the animals were followed only during the monthly consultation. The analyzed variable was the percentage of weight loss of each animal in one month of the method used. The animals of the daily assisted group had a mean weight loss of 5.17%, while the animalsof the non-assisted group had a mean weight loss of 3.31%. Considering the results, one can state that the animals in the assisted group lost approximately 1.6 times more weight than the unassisted animals, when comparing the mean and the median weight loss percentage in the two groups. Although the results are encouraging, there was no statistical difference with a significance level of 0.05, and this is probably due to the small sample size, consisting of 18 animals.
Show more [+] Less [-]Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate
2019
Mayara Ellen Bardi de Moraes | Paulo Roberto Adona | Samuel Guemra | Tiago Henrique Camara De Bem | Moysés dos Santos Miranda
The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.
Show more [+] Less [-]Predatory viability of the nematophagous fungus Duddingtonia flagrans on the infective larvae of gastrointestinal nematodes of sheep following storage and refrigeration
2019
Vinícius Longo Ribeiro Vilela | Paulo Wbiratan Loes da Costa | Francisca Flávia da Silva | Hermano Manoel Francisco Figueiredo Bezerra | Leonardo Vinicius Silva de Oliveira | Maysa de Oliveira Dantas | Thais Ferreira Feitosa | Jackson Victor de Araújo | Fabio Ribeiro Braga
The objective of this study was to evaluate the in vivo predatory viability of the nematophagous fungus, Duddingtonia flagrans, after storage (36 months) and refrigeration (2-8 °C). This viability was evaluated using the infective larvae of gastrointestinal nematodes of sheep in the Northeastern semi-arid region of Brazil. Sixteen Santa Inês sheep with negative counting of eggs per gram of feces (EPG) were divided into four experimental groups, each group comprised of four animals. The pellets were administered at the dose of 3 g/10 kg of live weight (20% fungal micelyum), and a single administration was performed for each animal. Group I was administered pellets that had been stored for 36 months; Group II, freshly produced pellets; Group III, freshly produced pellets that did not contain fungi; and Group IV, pellets were not administered, and this was the control group. Feces were collected for 5 days, every 24 h for analysis. There was a significant decrease in the number of infective larvae of sheep nematodes that received D. flagrans pellets in a sodium alginate matrix, 82% was observed for Group I and 71% for Group II, compared to the control group. It is therefore concluded that the fungus, D. flagrans, pelleted in sodium alginate matrix after 36 months of storage at 2-8 °C, showed efficacy in reducing the number of infective larvae of gastrointestinal nematodes of sheep.
Show more [+] Less [-]Detection of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii in horses, dogs and cat
2019
Marilia de Oliveira Koch | Luciane Maria Laskoski | Daniel Moura de Aguiar | Bianca Ressetti da Silva | Reinaldo Ramos Régio | Juliana Ikeda Ishikura | Frederico Fontanelli Vaz | Rosangela Locatelli-Dittrich
The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositive for S. neurona, N. caninum and T. gondii, respectively. No statistical difference was found between groups of seropositive dogs for the three protozoa with neurological signs and without neurological signs. Co-infection and high antibody titers were detected. The antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii were found widely distributed among horses, dogs and cats in the region of Curitiba, state of Paraná, Brazil.
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