Objective: The objective of this study was to investigate the efficacy of newly developed multi­component ear drops for the treatment of otitis caused by parasites, bacteria, and fungi in dogs, cats, ferrets, fancy rabbits, and foxes. Materials and Methods: A new drug for veterinary use was developed that contained levofloxacin hemihydrate (0.3%), clotrimazole (0.1%), dexamethasone sodium phosphate (0.1%), and mox­idectin (0.01%). In the initial stage of otoacariasis (or the ear form of psoroptic scab), the drug was used twice with an interval of 5–7 days. When otoacariasis (psoroptic scab) was complicated by otitis of bacterial and/or fungal etiology, the drug was used as follows: instilled once a day, within 10–14 days in the ear canal for 2–5 drops of the drug. The study of therapeutic efficacy was per­formed on cats, dogs, ferrets, foxes, and fancy rabbits with ear pathology. Results: The recovery of cats, ferrets, dogs, and foxes after the double use of ear drops during otoacariasis was established. In the ear form of psoroptic scab in fancy rabbits after treatment, Psoroptes cuniculi mites were not detected. The specific (antimicrobial and antifungal) activity of the drug was confirmed in vivo using the following scheme of drug use: the drug was instilled once a day, within 10–14 days in the ear canal, 2–5 drops of the drug in animals with external otitis and otitis media. Conclusion: The rational combination of active components developed in this study is effective and safe for use in animals with microbial diseases of the ears. J. Adv. Vet. Anim. Res., 7(1): 115-126, March 2020

Objective: Liver neoplasms are problematic among small domestic animals. The etiological cause of hepatocellular carcinomas in domestic animals is still unknown although it is believed that chronic infections and toxic substances can affect the development of this type of tumor. This study aimed to analyze the clinical and morphological characteristics of canine hepatocellular carcinoma. Materials and methods: In total, 6,958 cancer operations were performed in the clinic. Liver tumors were detected in 123 dogs in vivo and 375 dogs postmortem. All animals with suspected liver neoplasm were assessed, including history, clinical examination, complete blood count, biochemical blood tests, radiographic examination, and ultrasound with a biopsy for performing cytological and histological analyses. Results: Hepatocellular carcinomas have nonspecific clinical manifestations, also a characteristic aspect of other tumors of the hepatobiliary system. The hematological changes have an impact on the prognosis, and biochemical abnormalities reflect the changes in liver activity. The cytological diagnosis of hepatocellular tumors is difficult because of hepatocyte atypia in highly differentiated carcinomas. Finally, a histological examination was performed in all the dogs diagnosed with hepatocellular carcinoma. Conclusion: Hematological changes in dogs with hepatocellular carcinoma affect their prognosis. Biochemical abnormalities of this pathology reflect the changes in liver activity, not indicating a specific pathology. However, an increase in the activity of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase is an unfavorable prognostic sign. In this study, five of seven dogs with a tumor size of more than 5.0 cm had a life expectancy of 30, 51, and 91 days, suggesting that the size of the tumor is an adverse prognostic factor. J. Adv. Vet. Anim. Res., 7(1): 127-132, March 2020

Objective: This study was carried out to determine the frequency and prevalence of clinical con­ditions and their treatment, especially antibiotics in dogs and cats. Materials and Methods: A period of 12-month retrospective study was conducted at the Teaching Veterinary Hospital, Chattogram Veterinary and Animal Sciences University, Chittagong, Bangladesh from July 2018 to June 2019. A total of 849 cases, including 488(57.5%) and 361 (42.5%) dogs and cats respectively, were in account to espy the clinical conditions. Season, age, sex, and breed were the parameters to analyze the prevalence of those clinical conditions. Results: From the study, it was found that the endoparasitic infestation was highly frequent in both dog and cat (55% in dogs and 59% in cats). The endoparasitic infestation was highly preva­lent in cats (91.53%) significantly (p = 0.003), which were ≤1 year of age. On the other hand, the ectoparasitic infestation was found prevalent significantly (p = 0.06) in the winter than any season and dewormed dogs (p = 0.03). Prevalence of canine parvovirus infection in dogs and wound in cats were substantially higher (p < 0.001 and p=0.05 respectively) in the winter whereas the prev­alence of myiasis in dogs was prominent in the rainy season significantly (p = 0.01). The mostly used antibiotic was ceftriaxone (9.5% in dogs and 4% in cats). Conclusion: Different endoparasitic, ectoparasitic, and infectious diseases found prone to infect pet animals, mainly dogs, and cats. By maintaining proper anthelmintics and vaccine shots may act as a prevention procedure to those infections. J. Adv. Vet. Anim. Res., 7(1): 156-163, March 2020

Objective: This paper presents the obtained result of a study that realizes to associate a set of real and imputed single nucleotide polymorphisms (SNP) genotypes to the rear udder height in Holstein cows. Materials and methods: Forty-six Holstein cows from an arid zone of Mexico were phenotyped and genotyped for this study. Blood samples were used for DNA extraction, genotyping was per­formed with the Illumina BovineLD Bead chip which interrogates 6,912 SNPs genome-wide, and imputation was performed using the Findhap software. After QC filters, a total of 22,251 high quality and informative SNPs were inspected. Results: The results showed the detection of 76 significant SNPs throughout the complete genome. Significant SNPs fall inside 111 Quantitative Loci Traits related to protein percentage, milk yield, and fat, among others, in chromosomes 1, 2, 3, 5, 6, 9, 10, 11, 12, 13, 19, 20, 21, 23, 26, 27, and 29. Similarly, results confirm that a genotype imputation is a convenient option for genome-wide covering when selecting economic traits with low-density real SNP panels. Conclusion: This study contributes to establishing a low-cost and profitable strategy for applying genomic selection in developing countries. Adv. Vet. Anim. Res., 7(2): 234-241, June 2020

Objective: The purpose of the experiment was to examine the effect of different food sources on the growth and reproduction performances of an epigeic earthworm Perionyxexcavatus. Materials and Methods: The experiment was carried out in 18 cylindrical plastic containers for 10 weeks. The study was designed with six treatments, each having three replicates. In control treat­ment (T1), cow dung was used as the only food source for the earthworm. In another five treat­ments, water hyacinth (T2), chopped banana plant trunk (T3), vegetable scrap (T4), paddy straw (T5), and sugarcane bagasse (T6) were used as food sources with cow dung as bedding material. Results: The maximum weight gain of earthworm P. excavatuswas 3,294.7 ± 4.5 mg for the food staff of vegetable scrap (T4). Earthworm P. excavatusfed with chopped banana plant trunk (T3) showed a very similar weight gain of 3,243.7 ± 3.8 mg. On the contrary, the minimum weight gain was 1,799.7 ± 3.5 mg for the food staff of paddy straw (T5). The maximum cocoon number of 137.33 ± 6.46 mg was observed in T3, whereas a minimum number of 36.67 ± 4.16 mg in T1. The highest number of hatchlings (12.33 ± 0.88 mg) was recorded in treatment T3, whereas the least number of hatchling (5.00 ± 0.58 mg) was observed in T1. Conclusion: It was concluded that, among the six different food sources, chopped banana plant trunk was preferable food source to the earthworm for growth and reproduction. Adv. Vet. Anim. Res., 7(2): 331-337, June 2020

Objective: The aim of this research is to investigate the relationship level of oxytocin released and the tactile stimulation of the cervix in Holstein cattle from the northwestern arid regions in México.  Materials and Methods: The cervixes of 10 Holstein Friesian dairy cows in mid-lactation were manually stimulated for 60 sec. Oxytocin released in the blood was analyzed before and after stimulation. The enzyme-linked immunosorbent assay was used in the analysis to make oxytocin detectable by spectrometry techniques and by converting the luminescence absorbance of each sample into pg per mol.  Results: The study revealed that tactile stimulation of the cervix increased oxytocin levels. The highest increase in oxytocin level was 10,940.8222 pg/mol, while the lowest increase in oxytocin level was 1,830.94254 pg/mol. Besides, the milk production by tactile stimulation of the cervix had a higher milk yield and a p-value of 9.4 × 10−4 (p < 0.05) in comparison to the monthly average without tactile stimulation.  Conclusion: Tactile stimulation of the cervix activates the Ferguson reflex and increases the oxyto­cin released in the blood and increases the milk released from the alveolar glands. J. Adv. Vet. Anim. Res., 7(4): 608-613, Dec 2020 http://doi.org/10.5455/javar.2020.g458

Objectives: This study aimed to examine the effects of wet-to-dry bandages for the treatment of surgical wounds in goats. Materials and Methods: Three types of bandages, i.e., the calcium alginate gel, the homogenous platelet-rich plasma (PRP) gel dressing, and saline dressing, were used to promote the healing process for the management of wounds. Artificial circular wounds were created and treated with bandages to evaluate their effects on the wound healing process in 12 goats. The morphological parameters, i.e., diameters (cm), duration of healing, and wound contraction (cm), were evalu­ated to differentiate the postoperative sequelae on wound healing process up to day 30 in three different treatment groups of goats. Tissue specimens from the wounds were examined histo­pathologically on 1st, 3rd, 7th, and 30th postoperative days. Finally, the obtained data were ana­lyzed using the Statistical Package for the Social Sciences 19 software.  Results: The current study revealed that the contraction diameter was higher in calcium alginate gel and PRP gel treated group when compared to the saline dressing group. Aggregation of immu­noreactive cells (neutrophils, macrophages, and lymphocytes), collagen fiber bundles among the cells, and proliferation of blood vessels were observed in the epidermis of calcium alginate gel, PRP gel treated and saline dressing wounds, which promote the healing process of wounds. In visual inspection, the number of reactive cells and proliferation of blood vessels were higher in the calcium alginate gel and PRP gel treated group than the saline dressing.  Conclusion: In terms of epithelialization, epidermal characteristics, neovascularization, and infil­tration of immunoreactive cells, the calcium alginate gel and the homogenous PRP gel dressings showed the best healing performance. Therefore, the present study suggests that clinicians could consider the calcium alginate gel dressing and homogenous PRP gel dressing as beneficial for wound care. J. Adv. Vet. Anim. Res., 7(4): 647-654, Dec 2020 http://doi.org/10.5455/javar.2020.g463

Objective: Solubility and bioavailability are crucial for maximizing the activity of an antiparasitic drug. This study aimed to develop a combined preparation for antiparasitic medicines using iver­mectin (Iver), fenbendazole (FBZ), and triclabendazole (TBZ), considering their solubility, bioavail­ability, and activity.  Materials and Methods: Innovative preparations in solid dispersions (SD) were obtained using the joint mechanical processing of drug substances with polyvinylpyrrolidone (PVP) in an LE-101 roller mill. The preparations’ efficacy was studied in 140 sheep spontaneously infected with gastroin­testinal Strongylata, Dicrococelium dendriticum, Moniezia expansa, and Melophagus ovinus. The preparations were given individually to the sheep in the form of an aqueous suspension orally. Their effectiveness was evaluated using intravital and postmortem parasitological examinations.  Results: The results confirmed the increase in solubility of substances by 13–29 times. The exper­iments have shown the high efficacy of SD composition of FBZ/Iver/PVP (1/1/9) containing FBZ (at 3.0 mg/kg b/w) and Iver (at 0.2 mg/kg b/w) when used against gastrointestinal Strongylates and M. expansa (95.8% and 100%, respectively), to a lesser extent against M. ovinus (38.5%). The SD composition of TBZ/Iver/PVP (1/1/9) of TBZ (at 3.0 mg/kg b/w) and Iver (at 0.2 mg/kg b/w) showed a high efficacy against gastrointestinal Strongylata and D. dendriticum (96.8% and 100%, respectively) and less activity against M. ovinus (61.6%).  Conclusion: The high parasiticidal activity of SD based on FBZ, TBZ, and Iver in comparison with initial substances is explained by the formation of inclusion complexes of these substances with PVP when SD is dissolved in water and the synergistic effect of the active substances of the prepa­rations. The resulting complexes have increased solubility in water and bioavailability. The use of such an SD suggests a significant reduction in the dosages of FBZ and TBZ without losing parasit­icidal activity. J. Adv. Vet. Anim. Res., 7(4): 718-725, Dec 2020 http://doi.org/10.5455/javar.2020.g473

Objective: This study aimed to assess the organoleptic, physico-chemical, and microbial quality as well as the presence of any adulterants in different commercial and local milk samples. Materials and Methods: Three brands of ultra-high temperature (UHT) milk, three brands of pas­teurized milk, and three raw milk samples were procured and analyzed for different quality tests. Consumer preference about the milk samples were studied by evaluating the organoleptic prop­erties by a testing panel of 15 panelists. Physico-chemical, adulteration, and microbial analysis of the milk samples were performed by following different standard methods. Results: Most of the organoleptic properties were varied significantly at p ≤ 0.05 for UHT and pasteurized milk samples, while raw milk samples were equally acceptable to the panelists in most cases. From physico-chemical analysis, it was found that raw milk contained the highest amount of moisture (90.68%), whereas UHT had the lowest (87.60%), and other components were ranged as 0.68%–0.78% ash, 3.20%–3.58% protein, 3.15%–3.56% fat, 4.35%–4.62% lactose, 0.14%–0.22% acidity, 6.17%–8.95% solid not fat, 9.32%–12.40% total solid, and 1.026%–1.034% specific gravity. All adulteration tests responded negatively for raw samples, whereas commercial milk samples showed positive response only on added sugar test. Total standard plate count and coliform count tests showed that there was no microorganism in a detectable range in commer­cial milk samples, though raw samples had a significant amount. Conclusion: Though there were some fluctuations in some parameters of the three milk catego­ries, but this study concluded that the quality of UHT and pasteurized milk were excellent with respect to parameters studied. J. Adv. Vet. Anim. Res., 7(1): 26-33, March 2020

Objective: This study aimed to isolate and identify of pathogenic bacteria in tiny freshwater shrimp (Macrobrachium lanchesteri) and in Kung Ten, which is an unusual Thai cuisine that eaten alive shrimp directly. Antimicrobial susceptibility test and identification of antibiotic resistance genes for isolated bacteria were conducted. Materials and Methods: Eighty of fresh shrimp samples and forty of Kung Ten salads were collected from four fresh markets, which were located in Bangkok and Nonthaburi province (N = 120). The isolation, identification, and antimicrobial susceptibility test of pathogenic bacteria were done following the Clinical and Laboratory Standards Institute guidelines. Antibiotic-resistant bacteria were screened for β-lactamase relating genes, such as AmpC (MOX and ACC genes), blaCTX-M, and Int1 genes. Results: The number of bacterial isolates in tiny freshwater shrimp and Kung Ten salad was 136 and 65, respectively. Aeromonas caviae, A. hydrophilla, Proteus penneri, Proteus vulgaris, and Klebsiella pneumoniae were commonly found. Ampicillin, amoxicillin/clavulanic, cefuroxime, tet­racycline, and trimethoprim/sulfamethoxazole resistance were observed, and common antibiot­ic-resistant bacteria were A. caviae, P. vulgaris, Enterobacter Aerogenes, and K. pneumoniae. A. caviae, P. penneri, K. Pneumoniae, and A. hydrophilla were positive for MOX gene; blaCTX-M, and Int1 genes; ACC and Int1 genes; and ACC gene, respectively. Conclusion: Raw or uncooked shrimps in Kung Ten salad may a risk in foodborne diseases due to positive for pathogenic bacterial isolates. However, hygienic control on food preparation is diffi­cult to apply because of the difficulty of changing in local Thai food behavior. J. Adv. Vet. Anim. Res., 7(1): 83-91, March 2020