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Influence of housing systems on duck behavior and welfare Full text
2020
Hamid, Shereen El Abdel | Saleem, Al Sadik Y | Youssef, Mohamed I | Mohammed, Hesham H | Abdelaty, Asmaa I
Influence of housing systems on duck behavior and welfare Full text
2020
Hamid, Shereen El Abdel | Saleem, Al Sadik Y | Youssef, Mohamed I | Mohammed, Hesham H | Abdelaty, Asmaa I
Objective: This experiment was conducted to assess the effects of the housing system on the welfare and growth performance of the Muscovy duck. Materials and methods: A total number of 48 Muscovy duck aged 3-week old were divided randomly into two groups (24 duck on each): deep litter system and cage system. Each group was subdivided into three replicates (8 birds on each) were identified with wing rings. Results: The study showed that feeding and drinking behaviors significantly increased (p < 0.05) in duck reared at the cage system. While there was no noticeable effect on lying and feather pecking, duck raised in the deep litter had better growth performance with a substantial difference, which achieve a higher feed conversion rate with lower feed efficiency. Physical conditions were significantly better at cage management. The level of stress indicating hormones indicating free Thyroxin was increased with the cage. Therefore, the litter system improves duck welfare and their performance. Conclusion: It is concluded that the duck managed under the litter system has more opportunities that facilitate the expression of more natural behavior, and thus improve their health and performance, as a result of improved feed conversion rate and feed efficiency. J. Adv. Vet. Anim. Res., 7(3): 407-413, Sep 2020 http://doi.org/10.5455/javar.2020.g435
Show more [+] Less [-]Influence of housing systems on duck behavior and welfare Full text
2020
Shereen El. Abdel-Hamid | Al-Sadik Y. Saleem | Mohamed I. Youssef | Hesham H. Mohammed | Asmaa I. Abdelaty
Objective: This experiment was conducted to assess the effects of the housing system on the welfare and growth performance of the Muscovy duck. Materials and methods: A total number of 48 Muscovy duck aged 3-week old were divided ran¬domly into two groups (24 duck on each): deep litter system and cage system. Each group was subdivided into three replicates (8 birds on each) were identified with wing rings. Results: The study showed that feeding and drinking behaviors significantly increased (p < 0.05) in duck reared at the cage system. While there was no noticeable effect on lying and feather peck¬ing, duck raised in the deep litter had better growth performance with a substantial difference, which achieve a higher feed conversion rate with lower feed efficiency. Physical conditions were significantly better at cage management. The level of stress indicating hormones indicating free Thyroxin was increased with the cage. Therefore, the litter system improves duck welfare and their performance. Conclusion: It is concluded that the duck managed under the litter system has more opportunities that facilitate the expression of more natural behavior, and thus improve their health and perfor¬mance, as a result of improved feed conversion rate and feed efficiency. [J Adv Vet Anim Res 2020; 7(3.000): 407-413]
Show more [+] Less [-]Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles Full text
2020
Radwan Raheel, Ismail Abd Elhafez | Hassan, Walid Hamdy | Salem, Shaaban Salem Radwan | Salam, Hala Sayed Hassan
Objective: The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole-trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored blaTEM, sul1, tetA, qnrS, blaCTX-M, and fimH with a percentage of 100%, papC (40%), and hlyA (10%). FimH was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli associated with bovine endometritis. The detection of the fimH gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli. J. Adv. Vet. Anim. Res., 7(3): 442-451, Sep 2020 http://doi.org/10.5455/javar.2020.g440
Show more [+] Less [-]African swine fever in Benin and prevalence of the disease in Southern Benin: A retrospective study (2014–2018) Full text
2020
Ohouko, Okri Fréjus Hans | Koudouvo, Koffi | Dougnon, Tossou Jacques | Agbonon, Amegnona | Karim, Issaka Youssao Abdou | Farougou, Souaïbou | Gbeassor, Messanvi
Objective: This study aimed to assess the prevalence and risk factors of African swine fever (ASF) disease in Benin. Materials and methods: A retrospective study was conducted on 70 pig farms from the Departement of Atlantique and Ouémé and also by using the data available from the Directorate of Livestock on the spread of ASF in Benin from 2014 to 2018. The prevalence of ASF was assessed with 106 nasal swabs from apparently healthy domestic pigs and 15 organ samples from dead ASF-suspected pigs. ASF virus detection was carried out by conventional polymerase chain reaction using Qiagen Kit for DNA extraction. Data recorded were processed with SAS software (2006). Results: It appears that ASF is an endemic disease in Benin with the Department of Ouémé as the hotspot of dissemination of the virus in the country. The losses due to ASF recorded from 2014 to 2018 are evaluated to 884,850,000 CFA Franc by estimating the average cost of a pig at 25,000 FCFA. A prevalence of 1.89% (CI at 95%, 0.71–3.49) was recorded for live animals with a positive result in organs from all dead pigs suspected of ASF. Breeding practices related to the sharing of breeding males, scavenging pigs, and non-compliance with biosecurity measures were the risk factors identified. Conclusion: The present study sheds light on the areas prone to the ASF virus in Benin. Moreover, the cross-sectional data recorded on the prevalence of ASF will help to better rule on the spread of the disease. It would be interesting for the Beninese Republic to increase its efforts for ASF control. J. Adv. Vet. Anim. Res., 7(3): 464-470, Sep 2020 http://doi.org/10.5455/javar.2020.g442
Show more [+] Less [-]Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome Full text
2020
Prihatno, Surya Agus | Adi, Yosua Kristian | Budipitojo, Teguh
Objective: This study was carried out to find out the immunolocalization of Interleukin 6 (IL-6) and Interleukin 10 (IL-10) in the testicular tissue of testicular dysfunction rat treated with secretome from human umbilical stem cells. Materials and methods: Rats were induced with cisplatin for testicular dysfunction condition. After that, the rats were grouped into two categories and were treated with secretome at 0.2 and 0.5 ml/kg BW once every week for 4 weeks. One week later, after the secretome treatment, the rats were sacrificed for histological evaluation using the immunohistochemical method. The preparation slides were examined using a light microscope and were analyzed descriptively and quantitatively. Results: There were no IL-6 and IL-10 immunoreactivities seen in the testicular tissue after cisplatin induction. However, the immunoreactivities of IL-6 and IL-10 were detected after secretome treatment, with both dosages of 0.2 and 0.5 ml/kg BW. These immunoreactivities were detected in the spermatogonia, spermatid/luminal tissue of seminiferous tubule, spermatogenic cells, and Leydig cells. In the cell calculation, the numbers of IL-6 immunoreactive cells were higher at the lower secretome dosage. In contrast, the numbers of IL-10 immunoreactive cells were higher at the higher secretome dosage. Conclusion: IL-6 and IL-10 can be localized in the testicular tissue of testicular dysfunction rat after secretome treatment. J. Adv. Vet. Anim. Res., 7(3): 514-520, Sep 2020 http://doi.org/10.5455/javar.2020.g448
Show more [+] Less [-]Quality assessment and feeding impact of Moringa feed on intake, digestibility, enteric CH4 emission, rumen fermentation, and milk yield Full text
2020
Bashar, Muhammad Khairul | Huque, Khan Shahidul | Sarker, Nathu Ram | Sultana, Nasrin
Quality assessment and feeding impact of Moringa feed on intake, digestibility, enteric CH4 emission, rumen fermentation, and milk yield Full text
2020
Bashar, Muhammad Khairul | Huque, Khan Shahidul | Sarker, Nathu Ram | Sultana, Nasrin
Objective: This experiment was designed to assess the quality and to evaluate the feeding impact of moringa feed on intake, digestibility, rumen fermentation, methane (CH4) production, and milk yield. Materials and methods: According to body weight and exit–entry average daily milk production, fifteen BLRI cattle breed-1 lactating cows of 3rd or 4th stage of parturition with wk 3 and 4 of calving were selected and were equally and randomly distributed into three dietary groups. One group of cows was fed a control diet (T0) consisting of 1:1 dry matter (DM) of Napier silage and conventionally mixed concentrate. The other two groups were fed a control diet by randomly replacing i) 50% (T1) or ii) 100% (T2) of its concentrate with moringa feed. The three dietary groups were balanced nutritionally based on energy and protein following the Bangladesh Standards and Testing Institution (BSTI) standard. Results: The concentrate mixture was replaced with moringa feed to increase the feed efficiency and to reduce the DM or crude protein intake (p < 0.05) per 100 kg of metabolic body weight. The T2 group flourished with the highest (p < 0.05) amount of raw milk and also 4% fat-corrected milk (4.39 and 4.59 kg/day, respectively) compared to the T0 group (3.30 and 3.49 kg/day, respectively). However, it increased (p < 0.05) the concentration of total volatile fatty acid and decreased (p < 0.05) the blood and milk cholesterol, and ammonia-nitrogen (NH3-N) was reputed by adding moringa feed into the T0 group, without showing any significant (p > 0.05) change in CH4 production, fat, solid not fat, lactose or protein content of milk. Conclusion: Therefore, moringa feed increased the productivity in dairy cows, replacing the whole concentrate diet. J. Adv. Vet. Anim. Res., 7(3): 521-529, Sep 2020 http://doi.org/10.5455/javar.2020.g449
Show more [+] Less [-]Quality assessment and feeding impact of Moringa feed on intake, digestibility, enteric CH4 emission, rumen fermentation, and milk yield Full text
2020
Muhammad Khairul Bashar | Khan Shahidul Huque | Nathu Ram Sarker | Nasrin Sultana
Objective: This experiment was designed to assess the quality and to evaluate the feeding impact of moringa feed on intake, digestibility, rumen fermentation, methane (CH4) production, and milk yield. Materials and methods: According to body weight and exitentry average daily milk production, fifteen BLRI cattle breed-1 lactating cows of 3rd or 4th stage of parturition with wk 3 and 4 of calving were selected and were equally and randomly distributed into three dietary groups. One group of cows was fed a control diet (T0) consisting of 1:1 dry matter (DM) of Napier silage and conventionally mixed concentrate. The other two groups were fed a control diet by randomly replacing i) 50% (T1) or ii) 100% (T2) of its concentrate with moringa feed. The three dietary groups were balanced nutritionally based on energy and protein following the Bangladesh Standards and Testing Institution (BSTI) standard. Results: The concentrate mixture was replaced with moringa feed to increase the feed efficiency and to reduce the DM or crude protein intake (p < 0.05) per 100 kg of metabolic body weight. The T2 group flourished with the highest (p < 0.05) amount of raw milk and also 4% fat-corrected milk (4.39 and 4.59 kg/day, respectively) compared to the T0 group (3.30 and 3.49 kg/day, respec¬tively). However, it increased (p < 0.05) the concentration of total volatile fatty acid and decreased (p < 0.05) the blood and milk cholesterol, and ammonia-nitrogen (NH3-N) was reputed by adding moringa feed into the T0 group, without showing any significant (p > 0.05) change in CH4 produc¬tion, fat, solid not fat, lactose or protein content of milk. Conclusion: Therefore, moringa feed increased the productivity in dairy cows, replacing the whole concentrate diet. [J Adv Vet Anim Res 2020; 7(3.000): 521-529]
Show more [+] Less [-]The emergence of Caryospora neofalconis in falcons in Central Saudi Arabia Full text
2020
Alfaleh, Faleh | Alyousif, Mohamed | Elhaig, Mahmoud
Objective: This study aimed to examine the occurrence of Caryospora (C.) neofalconis in falcons from the central region of the Kingdom of Saudi Arabia (KSA). Materials and methods: Fecal samples (n = 149) from 149 healthy falcons including 56 saker falcons (Falco cherrug), 13 lanner falcons (F. biarmicus), 18 peregrine falcons (F. peregrinus), 40 Barbary falcons (F. pelegrinoides), and 22 gyrfalcons (F. rusticolus) were collected between October 2018 and May 2019. The fecal samples were examined for the presence of C. neofalconis by microscopic examination followed by confirmation by polymerase chain reaction targeting 18S rRNA genes and their phylogenetic analyses. Results: The overall prevalence of C. neofalconis in the falcons was recorded as 10.7% (16/149) by microscopic examination. The highest prevalence was found in F. peregrinus (6/18, 33.3%), followed by F. rusticolus (3/22, 13.6%), F. cherrug (5/56, 8.9 %) and F. pelegrinoides (2/40, 5.0%). There was no C. neofalconis infection observed in F. biamicus. The 18S rRNA gene could be amplified in eight samples. The phylogenetic analysis of two C. neofalconis isolates exhibited a close relationship with the Mexican isolate (KT03081) with a 99.7% identity. Conclusions: To our knowledge, based on the microscopic and molecular analysis, this is the first report of C. neofalconis in F. cherrug, F. rusticolus, F. pelegrinoides, and F. peregrinus from the central region of the KSA and it emphasize the value of adopting preventive measures to limit the spread of C. neofalconis. J. Adv. Vet. Anim. Res., 7(3): 530-536, Sep 2020 http://doi.org/10.5455/javar.2020.g450
Show more [+] Less [-]Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh Full text
2020
Islam, Md Saiful | Sabuj, Abdullah Al Momen | Haque, Zobayda Farzana | Pondit, Amrita | Hossain, Md Golzar | Saha, Sukumar
Objectives: The present study estimated the seroprevalence of avian reovirus (ARV) infections in backyard chickens of the Mymensingh district in Bangladesh. Materials and Methods: Considering several risk factors, a total of 460 serum samples were collected from backyard chickens from eight Upazilas of the Mymensingh district in Bangladesh. Blood samples were taken from the wing vein using 3-ml sterile syringes and kept at room temperature for clotting in a slanting position and then transported to the laboratory maintaining the cool chain. Subsequently, the prepared sera were harvested and stored at −20°C until used. Finally, an indirect enzyme-linked immunosorbent assay (ELISA) was performed to detect ARV-specific antibodies using a commercial ARV antibody detection ELISA test kit. Results: The results revealed high prevalence rates of ARV antibodies, with a total seroprevalence of 69.78% (321/460). Area-wise, 74.55% (82/110) seroprevalence was recorded as the highest in Mymensingh Sadar, whereas 64% (32/50) was the lowest in Gauripur Upazila. With regard to sex, female chickens showed a significantly higher (p < 0.05) seroprevalence as 90.33% (271/300) compared to male chickens 31.25% (50/160). With regard to age groups, the seroprevalence of ARV infection was 59.33% (89/150) within 2–8 weeks, 82% (205/250) within 9–16 weeks, and 45% (27/60) within 17–20 weeks, respectively. Based on hygienic conditions, the highest seroprevalence of ARV was noted in backyard chickens housed in poor conditions 80% (120/150) than good conditions 50% (40/80). Backyard chickens reared in free-ranging conditions exhibited a significantly higher seroprevalence 73.33% (220/300) of ARV antibodies compared to rearing in separate houses 63.12% (101/160). The seroprevalence of ARV was higher in crossbreeds 71.67% (43/60), brought from market 76% (38/50), and unhealthy 78.57% (55/70) backyard chickens than non-descriptive indigenous 69.5% (278/400), home-reared 69.02% (283/410), and healthy chickens 68.21% (266/390). Conclusion: The high prevalence of ARV antibodies revealed in the current study indicates an extensive exposure of ARV to backyard chickens in Bangladesh that may be transmitted naturally to other chickens, ultimately leading to ominous economic effects on the poultry sector. J. Adv. Vet. Anim. Res., 7(3): 546-553, Sep 2020 http://doi.org/10.5455/javar.2020.g452
Show more [+] Less [-]Canine atopic dermatitis attenuated by mesenchymal stem cells Full text
2020
Ramos, Fernanda de Oliveira | Malard, Patricia Furtado | Brunel, Hilana dos Santos Sena | Paludo, Giane Regina | Castro, Marcio Botelho de | da Silva, Paulo Henrique Sampaio | Vianna, Andre Rodrigues da Cunha Barreto | Lima, Eduardo Mauricio Mendes de
Objective: To evaluate the use of mesenchymal stem cells (MSCs) in the attenuation of canine atopic dermatitis (AD). Materials and methods: Sixteen dogs were selected and divided into three groups, mild, moderate, and severe, according to the Canine Atopic Dermatitis Extent and Severity Index (CADESI-4). They were evaluated for 82 days. The protocol recommended in this experiment was to inject 2 × 106/kg bodyweight of MSC’s in all groups by the intravenous route with intervals of applications of 21 days. The degree of pruritus was evaluated by examining the visual analog scale, the CADESI-4, the histopathology of the skin, hematological and biochemical parameters, the pyogenic effect of MSCs, and the thickness of the epidermis. Results: There was a significant difference in the reduction of epidermal thickness in the moderate and severe groups. Hematological, biochemical, and body temperature parameters remained within normal limits for the species with no side effects Conclusion: MSCs attenuated the clinical signs of AD. J. Adv. Vet. Anim. Res., 7(3): 554-565, Sep 2020 http://doi.org/10.5455/javar.2020.g453
Show more [+] Less [-]Quantitative ultrasound elastography and serum ferritin level in dogs with liver tumors Full text
2020
Huaijantug, Somkiat | Yatmark, Paranee | Phophug, Phummarin | Worapakdee, Mookrawee | Phutrakul, Alan | Julapanthong, Pruksa | Chuaychoo, Krittin
Quantitative ultrasound elastography and serum ferritin level in dogs with liver tumors Full text
2020
Huaijantug, Somkiat | Yatmark, Paranee | Phophug, Phummarin | Worapakdee, Mookrawee | Phutrakul, Alan | Julapanthong, Pruksa | Chuaychoo, Krittin
Objective: The objective of this study was to assess the serum ferritin level and quantitate ultrasound elastography as a marker to distinguish dogs with benign and malignant liver tumors. Materials and Methods: Twenty-eight dogs were determined the serum ferritin and ultrasound elastography by using fine-needle aspiration biopsy. Results: Our results demonstrated that dogs with malignant liver tumors had significantly higher mean serum ferritin concentrations than those with benign liver tumors (p = 0.004). The mean intensity of blue and red colors from elastography was greater in the malignant than those in the benign group, especially for the blue color, meaning that lesions showed more hard tissue. Additionally, histograms of blue color in the malignant tended to be higher than the benign group. Conclusion: We suggested that quantitative ultrasound elastography and serum ferritin concentration comprise an alternative and non-invasive diagnostic method that could be used to predict the type of liver tumors in dogs. J. Adv. Vet. Anim. Res., 7(4): 575-584, Dec 2020 http://doi.org/10.5455/javar.2020.g455
Show more [+] Less [-]Quantitative ultrasound elastography and serum ferritin level in dogs with liver tumors Full text
2020
Somkiat Huaijantug | Paranee Yatmark | Phummarin Phophug | Mookrawee Worapakdee | Alan Phutrakul | Pruksa Julapanthong | Krittin Chuaychoo
Objective: The objective of this study was to assess the serum ferritin level and quantitate ultra¬sound elastography as a marker to distinguish dogs with benign and malignant liver tumors. Materials and Methods: Twenty-eight dogs were determined the serum ferritin and ultrasound elastography by using fine-needle aspiration biopsy. Results: Our results demonstrated that dogs with malignant liver tumors had significantly higher mean serum ferritin concentrations than those with benign liver tumors (p = 0.004). The mean intensity of blue and red colors from elastography was greater in the malignant than those in the benign group, especially for the blue color, meaning that lesions showed more hard tissue. Additionally, histograms of blue color in the malignant tended to be higher than the benign group. Conclusion: We suggested that quantitative ultrasound elastography and serum ferritin concen¬tration comprise an alternative and non-invasive diagnostic method that could be used to predict the type of liver tumors in dogs. [J Adv Vet Anim Res 2020; 7(4.000): 575- 584]
Show more [+] Less [-]Differentiation of intraspecific phenotypic plasticity of elongate glassy perchlet, Chanda nama: Insights into landmark-based truss morphometric and meristic variations Full text
2020
Azad, Kamrun Naher | Mahfuj, Md Sarower E | Iqbal, Towsif | Azad, Khairun Naher | Shafaq, Md Arif Imtiaz
Differentiation of intraspecific phenotypic plasticity of elongate glassy perchlet, Chanda nama: Insights into landmark-based truss morphometric and meristic variations Full text
2020
Azad, Kamrun Naher | Mahfuj, Md Sarower E | Iqbal, Towsif | Azad, Khairun Naher | Shafaq, Md Arif Imtiaz
Objective: Understanding intraspecific phenotypic plasticity is a prerequisite to fish stock identification and sustainable fisheries management. In this study, we assessed intraspecific phenotypic plasticity in terms of meristic and morphometric characters of wild populations of elongate glassy perchlet, Chanda nama from two different rivers, namely Madhumati River – Narail (MRN) and Tulshiganga River – Jaypurhat (TRJ), and an ox-bow lake, Jhapa Baor – Jashore (JBJ) in Bangladesh. Materials and Methods: In this study, six meristics, 15 conventional morphometrics, and 23-truss-based morphometrics were subjected to a one-way analysis of variance (ANOVA), followed by the Tukey-HSD test. The mean values of three meristic counts and nine conventional and 12 truss-based morphometrics demonstrated significant differences in the ANOVA test. Furthermore, principal component analysis (PCA) and discrimination function analysis (DFA) were performed separately using conventional and truss-based morphometric data. Results: In PCA analysis, four principal components were extracted and cumulatively demonstrated 51.848%. On the contrary, two discriminant functions (DF1, 63.5%; DF2, 36.5%) resulted from DFA analysis. In the bi-plot alignment from the discriminant space, all individuals were exceedingly separated among the three inhabitants. A dendrogram developed using conventional and truss morphometric characters confirmed that two clusters were formed among three populations. The TRJ population formed a distinct cluster, and the JBJ population formed a different cluster with a subcluster of MRN. In the discriminant function analysis, precise classification outcomes displayed 82% of individuals into their unique populations, whereas 66.9% of individuals were categorized as a cross-validated assemblage. Conclusion: The baseline information resulting from the current study would be useful for environmental studies and further conservation of glassy perchlet populations in Bangladesh. J. Adv. Vet. Anim. Res., 7(4): 585-596, Dec 2020 http://doi.org/10.5455/javar.2020.g456
Show more [+] Less [-]Differentiation of intraspecific phenotypic plasticity of elongate glassy perchlet, Chanda nama: Insights into landmark-based truss morphometric and meristic variations Full text
2020
Kamrun Naher Azad | Md. Sarower-E- Mahfuj | Towsif Iqbal | Khairun Naher Azad | Md. Arif Imtiaz Shafaq
Objective: Understanding intraspecific phenotypic plasticity is a prerequisite to fish stock identifi¬cation and sustainable fisheries management. In this study, we assessed intraspecific phenotypic plasticity in terms of meristic and morphometric characters of wild populations of elongate glassy perchlet, Chanda nama from two different rivers, namely Madhumati River Narail (MRN) and Tulshiganga River Jaypurhat (TRJ), and an ox-bow lake, Jhapa Baor Jashore (JBJ) in Bangladesh. Materials and Methods: In this study, six meristics, 15 conventional morphometrics, and 23-truss-based morphometrics were subjected to a one-way analysis of variance (ANOVA), followed by the Tukey-HSD test. The mean values of three meristic counts and nine conventional and 12 truss-based morphometrics demonstrated significant differences in the ANOVA test. Furthermore, principal component analysis (PCA) and discrimination function analysis (DFA) were performed separately using conventional and truss-based morphometric data. Results: In PCA analysis, four principal components were extracted and cumulatively demon¬strated 51.848%. On the contrary, two discriminant functions (DF1, 63.5%; DF2, 36.5%) resulted from DFA analysis. In the bi-plot alignment from the discriminant space, all individuals were exceedingly separated among the three inhabitants. A dendrogram developed using conventional and truss morphometric characters confirmed that two clusters were formed among three pop¬ulations. The TRJ population formed a distinct cluster, and the JBJ population formed a different cluster with a subcluster of MRN. In the discriminant function analysis, precise classification out¬comes displayed 82% of individuals into their unique populations, whereas 66.9% of individuals were categorized as a cross-validated assemblage. Conclusion: The baseline information resulting from the current study would be useful for envi¬ronmental studies and further conservation of glassy perchlet populations in Bangladesh. [J Adv Vet Anim Res 2020; 7(4.000): 585-596]
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