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Results 1571-1580 of 22,132
Characterization of polypeptides synthesized and secreted by oviductal epithelial cell explants obtained from young, fertile and aged, subfertile mares.
1996
Brinsko S.P. | Ignotz G.G. | Ball B.A. | Thomas P.G.A. | Currie W.B. | Ellington J.E.
Oral administration of bovine lactoferrin for treatment of intractable stomatitis in feline immunodeficiency virus (FIV)-positive anad FIV-negative cats.
1996
Sato R. | Inanami O. | Tanaka Y. | Takase M. | Naito Y.
Pharmacokinetics, effects on renal function, and potentiation of atracurium-induced neuromuscular blockade after administration of a high dose of gentamicin in isoflurane-anesthetized dogs.
1996
Martinez E.A. | Mealey K.L. | Wooldridge A.A. | Mercer D.E. | Cooper J. | Slater M.R. | Hartsfield S.M.
Quantification of phenylbutazone in equine sera by use of high-performance liquid chromatography with a nonevaporative extraction technique.
1996
Peck K.E. | Ray A.C. | Manuel G. | Rao M.M. | Foos J.
Ultrasonography of the lungs, pleura, and mediastinum in healthy cows.
1996
Braun U. | Sicher D. | Pusterla N.
Effects of cisapride on feline colonic smooth muscle function.
1996
Washabau R.J. | Sammarco J.
Segmental variations of in vitro mechanical properties in equine superficial digital flexor tendons.
1996
Crevier N. | Pourcelot P. | Denoix J.M. | Geiger D. | Bortolussi C. | Ribot X. | Sanaa M.
Toxicity and kinetics of amitraz in dogs.
1996
Hugnet C. | Buronfosse F. | Pineau X. | Cadore J.L. | Lorgue G. | Berny P.J.
Influence of nocodazole on the development of donor blastomeres from 16-cell stage bovine embryos in nuclear transfer.
1995
Tanaka H. | Takahashi Y. | Hishinuma M. | Kanagawa H. | Kariya T.
The aim of the present study was to establish a reliable procedure with nocodazole treatment for the synchronous cleavage of blastomeres of bovine embryos used as nuclear donors for nuclear transfer. Sixteen-cell stage embryos derived from in vitro-maturation, fertilization and culture were used. In three initial experiments, embryos were incubated in mTCM-199 + FCS with various concentrations (0-20 mu-M) of nocodazole under 5% CO2 in air. The concentrations required to arrest the blastomeres in the mitotic phase were examined. The effects of 10 mu-M nocodazole were also examined by observation of the division rate of blastomeres after the removal of nocodazole. Ninety percent (90%) of the blastomeres were arrested in the mitotic phase when embryos were exposed to 10 and 20 mu-M nocodazole. Exposure to 10 mu-M nocodazole had the highest blastomere-cleavage rate (47%). When the exposure period to 10 mu-M nocodazole was prolonged to 36 hr, the division rate of the blastomeres decreased. Furthermore, the effects of 2 culture conditions (mTCM-199 under 5% CO2 in air vs modified synthetic oviduct fluid medium under 5% CO2, 5% O2 and 90% N2) were compared on the division rate of blastomeres of embryos exposed to 10 mu-M nocodazole for 12 hr. When the embryos were exposed to nocodazole in mSOF, the division rate of blastomeres was improved to about 60%. The blastomeres produced by this treatment condition were used as nuclear donors and the developmental potential of the reconstituted embryos was investigated. The developmental rate to the blastocyst stage was 30.1% (58/193). Five embryos were transferred to 5 recipient cows and 2 of the 5 recipients (40%) became pregnant. Subsequently, one normal calf was born.
Show more [+] Less [-]Adrenocortical function in neonatal and weanling Beagle pups.
1995
Randolph J.F. | Center S.A. | Reimers T.J. | Scarlett J.M. | Corbett J.R.
Adrenocortical function was assessed in 27 Beagle pups at 2, 4, 6, 8, 10, and 12 weeks of age by determination of plasma sodium, potassium, and chloride concentrations; serum aldosterone and cortisol concentrations; and plasma ACTH concentrations. Serum cortisol concentration was measured before and 1 and 2 hours after IM administration of 2.2 IU of ACTH/kg of body weight. Serum progesterone concentration also was determined for all pups at 2, 4, and 6 weeks of age. Mean baseline cortisol concentration was lower for pups 8 weeks old or younger than for mature dogs. Nevertheless, mean serum ACTH-stimulated cortisol concentration in dogs of all age groups increased into the adult reference range after administration of ACTH. For pups 4 weeks old or younger, increase in cortisol concentration was maximal at 2 hours after ACTH administration. However, in pups between 6 and 12 weeks of age, the increase in cortisol concentration was maximal 1 hour after ACTH administration in about a third of the pups, whereas the remaining pups had peak values at 2 hours. Mean plasma sodium, potassium, and chloride concentrations for each age group were within the reference ranges established for mature dogs, with the exception of lower mean plasma sodium and chloride concentrations in pups 4 weeks old or younger. Mean serum aldosterone concentration in pups of each age group was substantially higher than the range of aldosterone concentrations for clinically normal mature dogs. Median progesterone concentration was uniformly less than 0.2 ng/ml for all pups 6 weeks old or younger. The normal endogenous ACTH concentration and adequate cortisol responses to exogenous ACTH seen in our pups would support functional pituitary gland and adrenal cortex for cortisol production. The low baseline cortisol concentration observed in the pups of this study may be related to reduced binding of cortisol to plasma proteins, as exists in human infants.
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