Refine search
Results 171-180 of 447
Diagnostic imaging of isolated splenic torsion in two dogs
2007
Choi, J.H. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, H.W. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, J.K. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Jang, J.Y. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, J.Y. (Seoul National University, Seoul, Republic of Korea) | Yoon, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
Isolated splenic torsion is a rare disease, which is usually produced in deep-chest large breed dogs. A five-year old Pitbull terrier and a four-year old Yorkshire terrier were diagnosed as isolated splenic torsion with chronic form. Leukocytosis, anemia and elevated hepatic enzyme level were found in blood test and a large amount of ascites was observed. Abdominal radiography revealed splenomegaly and ascites and ultrasonography showed diffuse hypoechoic change of spleen in case 1 and hyperechoic change of spleen with focal hypoechoic regions in case 2, thromboembolism of splenic vein and abnormal direction of spleen.
Show more [+] Less [-]Upregulation of aquaporin 2 and aquaporin 4 in the water-deprived mongolian gerbil (Meriones unguiculatus) kidney
2007
Song, J.H. (Chungnam National University, Daejeon, Republic of Korea) | Kwon, J.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, Y.H. (Chungnam National University, Daejeon, Republic of Korea) | Park, Y.D. (Youngdong University, Youngdong, Republic of Korea) | Han, K.H. (Ewha Womans University, Seoul, Republic of Korea) | Ryu, S.Y. (Chungnam National University, Daejeon, Republic of Korea) | Jung, J.Y. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected]
Mongolian gerbil (Meriones unguiculatus) has been as an model animal for studing the neurological disease such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animal. Aquaporin 2 (AQP2) expressed at the surface of principal cells in collecting duct results from an equilibrium between the AQP2 in intracellular vesicles and the AQP2 on the plasma membrane.
Show more [+] Less [-]Functional characterization of P∧2x/P∧2Y receptor in isolated swine renal artery
2007
Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Jeon, J.C. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, S.K. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, S.J. (Inje University, Gimhae, Republic of Korea) | Lee, Y.G. (Inje University, Gimhae, Republic of Korea) | Won, J.Y. (Inje University, Gimhae, Republic of Korea) | Kang, J.S. (Inje University, Gimhae, Republic of Korea) | Hong, Y.G. (Inje University, Gimhae, Republic of Korea), E-mail: [email protected]
To understand the role of P∧2x/P∧2Y receptor in cortex region of kidney and renal artery, molecular and functional analysis of P∧2x/P∧2Y receptor by pharmacophysiological skill in conventional swine tissues were performed. In functional analysis of P∧2Y receptor for vascular relaxation, 2-methylthio adenosine triphosphate, a strong agonist of P∧2Y receptor, induced relaxation of noradrenaline (NA)-precontracted renal artery in a dose-dependent manner. Strikingly, relaxative effect of ATP, 2-msATP, agonists of P∧2Y receptor, abolished by treatment of reactive blue 2, a putative P∧2Y receptor antagonist.
Show more [+] Less [-]Development of Ultra-rapid Multiplex Real-time PCR for the Detection of Genes from Avian Influenza Virus subtype H5N1
2007
Kim, E.H. (Kyonggi University, Suwon, Republic of Korea) | Lee, D.W. (Kyonggi University, Suwon, Republic of Korea) | Han, S.H. (Kyonggi University, Suwon, Republic of Korea) | Lim, Y.K. (Cheju National University, Jeju, Republic of Korea) | Yoon, B.S. (Kyonggi University, Suwon, Republic of Korea), E-mail: [email protected]
Cause of high lethality and dissemination to human being, new development of rapid method for the detection of highly pathogenic Avian Influenza Virus (AIV) is still necessary. For the detection of AIV subtype H5N1, typical pathogenic AIV, new method to confirm sub-typing of this virus is also needed. For the purpose of ultra-rapid detection and sub-typing of hemagglutinin and neuraminidase of AIV, this study was planned. As the results we could demonstrate an ultra-rapid multiplex real-time PCR (URMRT-PCR) for the detection of AIV.
Show more [+] Less [-]Streptococcus equi subsp. zooepidemicus infection in barbary sheep (Ammotragus lervia)
2007
Koh, B.R.D. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea), E-mail: [email protected] | Park, S.D. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea) | Kim, J.I. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea) | Park, J.T. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea)
An eight years old female barbary sheep (Ammotragus lervia), which bred at the Gwangju Uchi Park Zoo had shown anorexia, depression,. respiratory problem for several weeks after parturition. In necropsy, extensive necrotizing pneumonia was found with severe immunocytes infiltration in the alveolar spaces and bronchioles. Pulmonary pleura were thickened with fibrin and inflammatory cells. Bacteria were isolated from lung and identified as Streptococcus equi subsp. zooepidemicus (SEZ) by biochemical tests and PCR on sodA and gusA genes, though seeⅠgene was not detected.
Show more [+] Less [-]Hemagglutinating encephalomyelitis virus infection in Korean suckling pigs
2007
Kim, E.M. (Seoul National University, Seoul, Republic of Korea) | Kim, H.K. (Seoul National University, Seoul, Republic of Korea) | Park, S.J. (Seoul National University, Seoul, Republic of Korea) | Lee, C.S. (Green Cross Veterinary Products, Yongin, Republic of Korea) | Luo, Yuzi (Seoul National University, Seoul, Republic of Korea) | Moon, H.J. (Seoul National University, Seoul, Republic of Korea) | Yang, J.S. (Seoul National University, Seoul, Republic of Korea) | Park, B.K. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
From January to June 2006, 54 suckling pigs had been submitted in virology lab., College of Veterinary Medicine, Seoul National University. All pigs had suffered from various symptoms such as respiratory, sign, enteric signs, neurologic signs, etc. Among 54 pigs, 24 pigs (44.4%) were positive for porcine hemagglutinating encephalomyelitis virus (HEV) through reverse transcription-nested polymerase chain reaction. According to this result, HEV infections seemed to be prevalent and widespread in Korean swine farms, and the infection is associated with respiratory signs and neurologic signs more than enteric signs.
Show more [+] Less [-]Beagle dogs parentage testing by using 22 ISAG microsatellite markers
2007
Ji, H.J. (Cheju National University, Jeju, Republic of Korea) | Kim, E.H. (Cheju National University, Jeju, Republic of Korea) | Lee, K.K. (Cheju National University, Jeju, Republic of Korea) | Kang, T.Y. (Cheju National University, Jeju, Republic of Korea) | Lee, J.M. (Cheju National University, Jeju, Republic of Korea) | Shin, H.D. (SNP Genetics, Inc., Seoul, Republic of Korea) | Kim, L.H. (SNP Genetics, Inc., Seoul, Republic of Korea) | Yun, Y.M. (Cheju National University, Jeju, Republic of Korea), E-mail: [email protected]
The objective of the study was to establish routine parentage testing system in Beagle dogs using 22 ISAG (International Society for Animal Genetics) canine microsatellite markers (2005). Blood collections were obtained from a mother dog, 4 candidate father dogs and 3 offspring (n = 8). Genomic DNA samples were extracted from 8 Beagle dogs blood for PCR analysis. PCR products for the allele were analyzed by ABI 3130 DNA Sequencer and GeneScan (Ver 3.0) analysis and Genotyper (Ver. 2.1) software.
Show more [+] Less [-]<i>Culicoides</i> biting midges at the National Zoological Gardens of South Africa : research communication Full text
2007
K. Labuschagne | L.J. Gerber | I. Espie | S. Carpenter
<i>Culicoides</i> biting midges at the National Zoological Gardens of South Africa : research communication Full text
2007
K. Labuschagne | L.J. Gerber | I. Espie | S. Carpenter
Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the transmission of a large number of pathogens to livestock and wild animals. In this study the presence of the genus, using light traps based at four different sites within the National Zoological Gardens of South Africa, was investigated during 2002-2004. In total, 37 species were recorded, including large numbers of Culicoides imicola Kieffer, 1913, which is responsible for the transmission of economically important arboviruses in South Africa, Europe, Middle and Far East. These results are discussed with reference to the wider Culicoides fauna in the Onderstepoort area of South Africa, their vector competence as well as biosecurity at the National Zoological Gardens.
Show more [+] Less [-]Culicoides biting midges at the National Zoological Gardens of South Africa : research communication Full text
2007
Labuschagne, K. | Gerber, L.J. | Espie, I. | Carpenter, S.
Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the transmission of a large number of pathogens to livestock and wild animals. In this study the presence of the genus, using light traps based at four different sites within the National Zoological Gardens of South Africa, was investigated during 2002-2004. In total, 37 species were recorded, including large numbers of Culicoides imicola Kieffer, 1913, which is responsible for the transmission of economically important arboviruses in South Africa, Europe, Middle and Far East. These results are discussed with reference to the wider Culicoides fauna in the Onderstepoort area of South Africa, their vector competence as well as biosecurity at the National Zoological Gardens.
Show more [+] Less [-]Intramammary antibiotics in dairy goats : withdrawal periods of three intramammary antibiotics compared to recommended withdrawal periods for cows Full text
2007
J. Karzis | E.F. Donkin | I.M. Petzer
Intramammary antibiotics in dairy goats : withdrawal periods of three intramammary antibiotics compared to recommended withdrawal periods for cows Full text
2007
J. Karzis | E.F. Donkin | I.M. Petzer
Intramammary antibiotics are registered and tested for use in dairy cattle. This study investigated withdrawal periods of three intramammary antibiotics (Curaclox LC [Norbrook Pharmacia AH]), Spectrazol Milking Cow (Schering-Plough Animal Health) and Rilexine 200 LC (Logos Agvet [Virbac]) in dairy goats and compared them to withdrawal periods recommended for use in cattle. Three trials were carried out in two different herds. The withdrawal periods for Curaclox LC in eight relatively low producing Saanen goats were 74.3 h (+ 19.21) measured by Thermo-Resistant Inhibitory Substances (TRIS), 90.0 h (+ 16.97) measured by colour dye, 99.4 h (+ 9.07) for cloxacillin measured by Parallux Beta Lactam Assay test (IDEXX distributors), and 92.6 h (+ 11.41) for ampicillin measured by Parallux. The withdrawal period for Curaclox LC recommended for use in cattle (72 h) was significantly shorter than the withdrawal periods as measured by colour dye (P < 0.001), Parallux testing for cloxacillin (P < 0.001) and ampicillin (P = 0.003). There was a significant difference in withdrawal periods as measured by TRIS (P = 0.009) and colour dye (P = 0.036). The mean withdrawal periods measured on 12 relatively high producing Saanen and Saanen-Toggenburg crossbreed dairy goats measured by TRIS, colour dye and Parallux for ampicillin and cloxacillin were, however, shorter at 42.0 h (+ 7.077), 64.5 h (+ 60.26), 77.3 h (+ 13.56) and 70.7 h (+ 12.65), respectively. These withdrawal periods for Curaclox LC were significantly longer than the withdrawal periods recommended for cattle as measured by TRIS (P < 0.001) and colour dye (P < 0.001). The combined withdrawal periods for Curaclox LC in the two trials as measured by TRIS, colour dye, Parallux testing for Cloxacillin and Ampicillin were 58.64h (+ 24.31), 75.8 h (+ 17.70), 87.0 h (+ 16.10) and 80.3 h (+ 16.23), respectively. The withdrawal period, when data of the two trials were combined, measured by TRIS (P < 0.001) was significantly longer than the withdrawal period recommended for use in cattle. The mean withdrawal period as measured by TRIS for Spectrazol Milking Cow for seven relatively low producing Saanen dairy goats was 95.3 h (+ 17.23). This was significantly (P < 0.001) longer than the withdrawal period recommended for use in cattle (60 h) for Spectrazol Milking Cow. The mean withdrawal period as measured by TRIS for Rilexine 200 LC for 20 relatively high producing Saanen and Saanen-Toggenburg crossbreed dairy goats was 36.9 h (+ 9.943) and was significantly (P < 0.001) shorter than the withdrawal period as recommended for use in cattle (96 h). There were also significant differences in all the one-sample t-tests between withdrawal periods for goats and recommended withdrawal periods for cattle without the 24 h safety margin.
Show more [+] Less [-]Intramammary antibiotics in dairy goats : withdrawal periods of three intramammary antibiotics compared to recommended withdrawal periods for cows Full text
2007
Karzis, J. | Donkin, E.F. | Petzer, I.M.
Intramammary antibiotics are registered and tested for use in dairy cattle. This study investigated withdrawal periods of three intramammary antibiotics (Curaclox LC [Norbrook Pharmacia AH]), Spectrazol Milking Cow (Schering-Plough Animal Health) and Rilexine 200 LC (Logos Agvet [Virbac]) in dairy goats and compared them to withdrawal periods recommended for use in cattle. Three trials were carried out in two different herds. The withdrawal periods for Curaclox LC in eight relatively low producing Saanen goats were 74.3 h (+ 19.21) measured by Thermo-Resistant Inhibitory Substances (TRIS), 90.0 h (+ 16.97) measured by colour dye, 99.4 h (+ 9.07) for cloxacillin measured by Parallux Beta Lactam Assay test (IDEXX distributors), and 92.6 h (+ 11.41) for ampicillin measured by Parallux. The withdrawal period for Curaclox LC recommended for use in cattle (72 h) was significantly shorter than the withdrawal periods as measured by colour dye (P 0.001), Parallux testing for cloxacillin (P 0.001) and ampicillin (P = 0.003). There was a significant difference in withdrawal periods as measured by TRIS (P = 0.009) and colour dye (P = 0.036). The mean withdrawal periods measured on 12 relatively high producing Saanen and Saanen-Toggenburg crossbreed dairy goats measured by TRIS, colour dye and Parallux for ampicillin and cloxacillin were, however, shorter at 42.0 h (+ 7.077), 64.5 h (+ 60.26), 77.3 h (+ 13.56) and 70.7 h (+ 12.65), respectively. These withdrawal periods for Curaclox LC were significantly longer than the withdrawal periods recommended for cattle as measured by TRIS (P 0.001) and colour dye (P 0.001). The combined withdrawal periods for Curaclox LC in the two trials as measured by TRIS, colour dye, Parallux testing for Cloxacillin and Ampicillin were 58.64h (+ 24.31), 75.8 h (+ 17.70), 87.0 h (+ 16.10) and 80.3 h (+ 16.23), respectively. The withdrawal period, when data of the two trials were combined, measured by TRIS (P 0.001) was significantly longer than the withdrawal period recommended for use in cattle. The mean withdrawal period as measured by TRIS for Spectrazol Milking Cow for seven relatively low producing Saanen dairy goats was 95.3 h (+ 17.23). This was significantly (P 0.001) longer than the withdrawal period recommended for use in cattle (60 h) for Spectrazol Milking Cow.The mean withdrawal period as measured by TRIS for Rilexine 200 LC for 20 relatively high producing Saanen and Saanen-Toggenburg crossbreed dairy goats was 36.9 h (+ 9.943) and was significantly (P 0.001) shorter than the withdrawal period as recommended for use in cattle (96 h). There were also significant differences in all the one-sample t-tests between withdrawal periods for goats and recommended withdrawal periods for cattle without the 24 h safety margin.
Show more [+] Less [-]Red blood cell volume as a predictor of fatal reactions in cattle infected with <i>Theileria parva</i> Katete Full text
2007
P. Fandamu | T. Marcotty | J.R.A. Brandt | L. Duchateau | N. Speybroeck | T.T. Dolan | D. Berkvens
Red blood cell volume as a predictor of fatal reactions in cattle infected with <i>Theileria parva</i> Katete Full text
2007
P. Fandamu | T. Marcotty | J.R.A. Brandt | L. Duchateau | N. Speybroeck | T.T. Dolan | D. Berkvens
A comparison of mean corpuscular volume (MCV) and packed cell volume (PCV) was made between cattle undergoing lethal and non-lethal reactions following experimental infections with the apicomplexan protozoa, Theileria parva Katete. This work confirmed that anaemia occurs in infected animals. However, the fall in PCV was steeper in lethal reactions compared to non-lethal reactions. Our results show that animals with initially lower MCV values are more prone to fatal reaction, despite having normal PCV profiles. The study also found that small red blood cells are more likely to be infected with T. parva. These findings suggest that animals with a higher proportion of small red blood cells in circulation will be more likely to succumb to T. parva infections. The potential for using MCV as a predictor of the outcome of infection challenge is discussed.
Show more [+] Less [-]Red blood cell volume as a predictor of fatal reactions in cattle infected with Theileria parva Katete Full text
2007
Fandamu, P. | Marcotty, T. | Brandt, J.R.A. | Duchateau, L. | Speybroeck, N. | Dolan, T.T. | Berkvens, D.
A comparison of mean corpuscular volume (MCV) and packed cell volume (PCV) was made between cattle undergoing lethal and non-lethal reactions following experimental infections with the apicomplexan protozoa, Theileria parva Katete. This work confirmed that anaemia occurs in infected animals. However, the fall in PCV was steeper in lethal reactions compared to non-lethal reactions. Our results show that animals with initially lower MCV values are more prone to fatal reaction, despite having normal PCV profiles. The study also found that small red blood cells are more likely to be infected with T. parva. These findings suggest that animals with a higher proportion of small red blood cells in circulation will be more likely to succumb to T. parva infections. The potential for using MCV as a predictor of the outcome of infection challenge is discussed.
Show more [+] Less [-]