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Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea
2011
Park, H.S., Kyungpook National University, Daegu, Republic of Korea | Lim, I.S., Kyungpook National University, Daegu, Republic of Korea | Kim, S.K., Kyungpook National University, Daegu, Republic of Korea | Kim, T.K., Gyeongnam Livestock Promotion Institute, Jinju, Republic of Korea | Yeo, S.G., Kyungpook National University, Daegu, Republic of Korea
Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at 50℃ for 30 min, but labile to 100% ethanol, heat at 52~60℃ for 30 min, 1 M MgCl₂ at 50℃ for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Show more [+] Less [-]Glatiramer acetate inhibits the activation of NFκB in the CNS of experimental autoimmune encephalomyelitis
2011
Hwang, I.S., Jeju National University, Jeju, Republic of Korea | Ha, D.B., Jeju National University, Jeju, Republic of Korea | Kim, D.S., Jeju National University, Jeju, Republic of Korea | Joo, H.J., Jeju National University, Jeju, Republic of Korea | Jee, Y.H., Jeju National University, Jeju, Republic of Korea
Glatiramer acetate (GA; Copaxone) has been shown to be effective in preventing and suppressing experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis (MS). It has been recently shown that GA-reactive T cells migrate through the blood-brain barrier, accumulate in the central nervous system (CNS), secrete antiinflammatory cytokines and suppress production of proinflammatory cytokines of EAE and MS. Development of EAE requires coordinated expression of a number of genes involved in the activation and effector functions of inflammatory cells. Activation of inflammatory cells is regulated at the transcriptional level by several families of transcription factors. One of these is the nuclear factor kappa B (NFκB) family which is present in a variety of cell types and involved in the activation of immune-relative genes during inflammatory process. Since it is highly activated at site of inflammation, NFκB activation is also implicated in the pathogenesis of EAE. In this study, we examined whether the inhibition of NFκB activation induced by GA can have suppressive therapeutic effects in EAE mice. We observed the expression of NFκB and phospho-IκB proteins increased in GA-treated EAE mice compared to EAE control groups. The immunoreactivity in inflammatory cells and glial cells of NFκB and phospho-IκB significantly decreased at the GA-treated EAE mice. These results suggest that treatment of GA in EAE inhibits the activation of NFκB and phophorylation of IκB in the CNS. Subsequently, the inhibition of NFκB activation and IκB phosphorylation leads to the anti-inflammatory effects thereby to reduce the progression and severity of EAE.
Show more [+] Less [-]The impact of the duration of retained placenta on postpartum diseases and culling rates in dairy cows
2011
Hur, T.Y., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Jung, Y.H., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Kang, S.J., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Choe, C.Y., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Kim, U.H., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Ryu, I.S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Son, D.S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Park, S.J., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Kim, I.H., Chungbuk National University, Cheongju, Republic of Korea
The objective of this study was to evaluate the duration of retained placenta (RP) on postpartum diseases and culling in dairy cows. Data were collected from 456 Holstein cows between 2008 and 2010. RP (greater-than or equal to 24 h after parturition) and postpartum diseases were diagnosed by farm managers and a veterinarian according to standardized definitions. The overall incidence rate of RP was 14.0%, and the incidence rate of RP with fourth and higher parity was two times that of cows having offspring for the first time. The duration of RP was 2 to 15 days with a mean of 6.8 days, except for cases of fourth and higher parity which had a mean of 10.5 days. A total of 63% of cows with RP had postpartum diseases. Among the cows diagnosed with RP, 23.4% developed metritis, and of those, 35.3% developed endometritis or pyometra. A total of 25% (n = 16) cows with RP were culled within 60 days in milk (DIM) and of those culled, 75% (n = 12) had postpartum diseases. These results suggest that RP increases the risk of postpartum diseases such as metritis and mastitis and is a culling hazard up to 60 DIM.
Show more [+] Less [-]Aerobic bacteria from oral cavities and cloaca of snakes in a petting zoo
2011
Jho, Y.S., Snoopy Animal Hospital, Gunpo, Republic of Korea | Park, D.H., Toxicology Center, Korea Institute of Toxicology, Daejeon, Republic of Korea | Lee, J.H., Toxicology Center, Korea Institute of Toxicology, Daejeon, Republic of Korea | Lyoo, Y.S., Konkuk University, Seoul, Republic of Korea
It is important to identify the bacteria in snakes because they can cause disease; importantly, bacteria such as Stenotrophomonas maltophilia, Escherichia coli, Proteus vulgaris etc. could be pathogens especially in hospitalized, debilitated hosts, and immunocompromised patients. To analyze the distribution of snakes' bacteria in petting zoo, samples from 20 snakes were collected from 2002 to 2008. Nine bacteria species were isolated from both oral and cloaca while four and six species were identified only from oral and cloaca, respectively. Except for Actinobacter sp., all of the identified strains are opportunistic pathogens, and most of them can cause nosocomial infections in humans. Present results indicate that prevalence of various zoonotic bacterial strains in snakes could be involved in potential transfer of these bacteria into caretakers and other animals. Therefore, it needs to examine the antibiotic resistance of these pathogens to prevent outbreaks.
Show more [+] Less [-]An animal model using Eimeria live vaccine and to study coccidiosis protozoa pathogenesis
2011
Lee, H.A., Wonkwang University, Iksan, Republic of Korea | Hong, S.H., Wonkwang University, Iksan, Republic of Korea | Choe, O.M., Wonkwang University, Iksan, Republic of Korea | Kim, O.J., Wonkwang University, Iksan, Republic of Korea
Cell culture systems for the protozoan Eimeria are not yet available. The present study was conducted to develop an animal model system by inoculating animals with a live Eimeria vaccine. This study was conducted on 3-day-old chickens (n = 20) pretreated with cyclophosphamide. The chickens were divided into 2 groups: the control group (n = 10) and the inoculated group that received the live Eimeria vaccine (n = 10). During the study period, we compared the clinical signs, changes in body weight, and number of oocysts shed in the feces of the control and inoculated group. This study showed that oocyst shedding was significantly higher in the chickens inoculated with live Eimeria oocysts than in the control chickens. Moreover, body weight gain was lesser in the animals in the inoculated group than in the control animals. Fecal oocyst shedding was observed in the inoculated animals. On the basis of these findings, we suggest that live Eimeria vaccination with cyclophosphamide pretreatment may be used to obtain an effective animal model for studying protozoan infections. This animal study model may eliminate the need for a tedious continuous animal inoculation process every 6 months because the live coccidiosis vaccine contains live oocysts.
Show more [+] Less [-]Protective effect of ethyl acetate extract of Ishige okamurae against carbon tetrachloride-induced acute liver injury in rats
2011
Kang, S.H., Jeju National University, Jeju, Republic of Korea | Yang, W.J., Jeju National University, Jeju, Republic of Korea | Oh, H.S., Jeju National University, Jeju, Republic of Korea | Bae, Y.J., Jeju National University, Jeju, Republic of Korea | Ahn, M.J., Jeju National University, Jeju, Republic of Korea | Kang, M.C., Jeju Technopark, Jeju, Republic of Korea | Ko, R.K., Jeju Technopark, Jeju, Republic of Korea | Kim, G.O., Jeju Technopark, Jeju, Republic of Korea | Lee, J.H., Samsung Changwon Hospital, School of Medicine, Sungkyunkwan University, Changwon, Republic of Korea | Hyun, J.W., Jeju National University, Jeju, Republic of Korea | Moon, C.J., Chonnam National University, Gwangju, Republic of Korea | Shin, T.K., Jeju National University, Jeju, Republic of Korea
Several compounds and extracts isolated from a brown alga, Ishige (I.) okamurae, exhibit anti-oxidant and anti-inflammatory effects. The present study investigated whether the ethyl acetate (EtOAc) fraction of I. okamurae (EFIO) could ameliorate carbon tetrachloride (CCl₄)-induced hepatotoxicity in rats. Sprague-Dawley rats were intraperitoneally (i.p.) administered with EFIO at 10 or 50 mg/kg per day for 2 consecutive days before CCl₄ injection (3.3 mL/kg, i.p.). Twenty four hours later, the rats were anesthesized with diethyl ether and dissected. Pretreatment with EFIO significantly reduced the increased serum levels of alanine aminotransferase and aspartate aminotransferase in CCl₄-treated rats. Pretreatment with EFIO also significantly inhibited the reduced activities of superoxide dismutase and catalase in the CCl₄-injured liver. Histopathological evaluations showed that hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration induced by CCl₄ treatment were ameliorated by the administration of EFIO. Additionally, liver immunohistochemical analyses revealed the marked reduction in ED1-positive monocyte-like macrophages in EFIO-pretreated rats given CCl₄. These results suggest that EFIO ameliorates CCl₄-induced liver injury, possibly through the inhibition of oxidative stress.
Show more [+] Less [-]Awareness effects by aquapuncture with dexamethasone in tiletamine/zolazepam anesthetized dogs
2011
Park, H.J., Chungnam National University, Daejeon, Republic of Korea | Song, K.H., Chungnam National University, Daejeon, Republic of Korea | Kim, D.H., Chungnam National University, Daejeon, Republic of Korea
Awareness effect of aquapuncture with dexamethasone on GV-26 was studied in tiletamine/zolazapam anesthetized six healthy crossbred dogs using a randomized cross-over experimental study design. After anesthesia by tiletamine/zolazapam, 0.1 mg dexamethasone was injected on GV-26 at 20 min in the experimental group. For the control group, 0.1 mg dexamethasone was injected on the quadriceps femoralis. A significant difference was evident in the sedation score between groups at 60 and 90 min (p less than 0.05). A significant difference was also evident in the analgesia score at 50 and 60 min (p less than 0.05). At 90 min, all dogs in the experimental group responded strongly to pain (p less than 0.01). Head up time, sternal recumbent time, standing time, and walking time was shorter in the experimental group, but the difference from the control group was statistically significant only for head up time and walking time (p less than 0.05). GV-26 acupuncture with dexamethasone is useful for awareness effects after anesthesia.
Show more [+] Less [-]Central diabetic insipidus associated with suspected pituitary gland tumor in a dog
2011
Lee, K.I., Konkuk University, Seoul, Republic of Korea | Park, H.M., Konkuk University, Seoul, Republic of Korea
A 12-year-old spayed female Yorkshire terrier dog was presented with two-weeks history of lethargy, mental dullness and polydipsia. Neurologic examination revealed proprioceptive defect of all limbs and loss of swallowing gag reflex. The dog revealed persistent dehydration, hypernatremia, hyperosmolarity and hyposthenuria. On magnetic resornance imaging (MRI), the mass were heterogeneous signality on T1 weighted images, hyperintense signality on T2 weighted image with contrast enhancement on hypothalamohypophyseal lesion. Based on these findings, the dog was suspected as having pituitary gland tumor. Through water deprivation test and response to desmopressin acetate (1-deamino-8-Darginine, DDAVP), this case was diagnosed by central diabetes insipidus (CDI). This paper reports the clinical sign, MRI, response to the exogenous antidiuretic hormone of CDI due to suspected pituitary tumor in a dog and DDAVP administration was evaluated effective therapy to correct hypernatremia induced by CDI.
Show more [+] Less [-]Comparison of bacterial culture, polymerase chain reaction, and a mix-enzyme-linked immunosorbent assay for the detection of Salmonella status in grow-to-finish pigs in western Canada with a Bayesian approach
2011
Wilkins, Wendy | Waldner, Cheryl | Rajić, Andrijana | McFall, Margaret | Chow, Eva | Muckle, Anne
Among grow-to-finish pigs from 10 herds in Alberta and Saskatchewan, 23 (16%) of 144 fecal samples were culture-positive and 40 (28%) of 144 pigs were seropositive for Salmonella. With a Bayesian model specifying dependence between the 2 tests, the sensitivity (Se) of culture and real-time polymerase chain reaction (RT-PCR) was 79% to 86%, depending on the cut-off value for the enzyme-linked immunosorbent assay (ELISA). Culture specificity (Sp) was assumed to be 100%; RT-PCR Sp was found to be 94%. The ELISA Se was 76% and 51% at optical density cut-off values ≥ 20% and ≥ 40%, respectively; the Sp was 94% at each cut-off value. The model showed some sensitivity to ELISA prior information, the ELISA Se being approximately 8% lower when informative prior information was specified in the model. When there was no adjustment for dependence between culture and RT-PCR, the posterior estimates for both culture and RT-PCR Se were 11% higher than with the conditional-dependence model and had considerably narrower probability intervals, which suggests that correlation between culture and PCR is important and should be adjusted for in future studies.
Show more [+] Less [-]Effects of hyperbaric oxygen treatment on horses with experimentally induced endotoxemia
2011
Baumwart, Chad A. | Doherty, Tom J. | Schumacher, James | Willis, Rebekah S. | Adair, Henry S III | Rohrbach, Barton W.
Objective: To determine the effectiveness of preinduction hyperbaric oxygen treatment (HBOT) in ameliorating signs of experimentally induced endotoxemia in horses. Animals: 18 healthy adult horses. Procedures: Horses were randomly assigned to 1 of 3 equal-sized treatment groups to receive normobaric ambient air and lipopolysaccharide (LPS), HBOT and LPS, or HBOT and physiologic saline (0.9% NaCl) solution. Horses were physically examined, and blood was obtained for a CBC and to determine concentration or activity of plasma tissue necrosis factor-α, blood lactate, and blood glucose before the horses were treated with HBOT and then intermittently for 6 hours after administration of LPS or physiologic saline solution. Results: All LPS-treated horses developed signs and biochemical and hematologic changes consistent with endotoxemia. Treatment with HBOT significantly ameliorated the effect of LPS on clinical endotoxemia score but did not significantly improve other abnormalities associated with endotoxemia. Conclusions and Clinical Relevance: The protective effect of HBOT was minimal, and results did not support its use as a treatment for horses prior to development of endotoxemia.
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