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Study of troponin, creatine kinase biomarkers, and histopathological lesions in experimental Nerium oleander toxicity in rats and mice
2018
Khordadmehr Monireh | Nazifi Saeed
Nerium oleander is a plant of the Apocynaceae family toxic to humans, animals, and insects. This study was performed to determine the cardiac and neurotoxicity of the plant extract by oral administration in Wistar rats and Balb/c mice and to compare the susceptibility of these animal models to oleander toxicity.
Show more [+] Less [-]Plasma concentration of norepinephrine, β-endorphin, and substance P in lame dairy cows
2018
Rodriguez Alfredo Rosamel | Herzberg Daniel Eduardo | Werner Marianne Patricia | Müller Heine Yacob | Bustamante Hedie Almagro
Lameness is a painful and debilitating condition that affects dairy cows worldwide. The aim of this study was to determine the plasma concentration of norepinephrine, β-endorphin, and substance P in dairy cows with lameness and different mobility scores (MS).
Show more [+] Less [-]Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus
2018
Yasmin Farkhanda | Yaqub Tahir | Idrees Muhammad | Shahzad Wasim | Hashmi Abu Saeed | Aqil Kiran | Mukhtar Nadia | Zahoor Muhammad Yasir | Akhtar Naeem | Umar Sajid
Dengue is one of the major emerging viral diseases in the world, with dramatic increases in reported cases in the last few decades and annual worldwide occurrence of approximately 390 million infections. It is a highly important mosquito-vectored disease and is a problem in tropical and subtropical areas of the world. The major aim of this study was to clone and express the dengue NS3 gene, in service to its therapeutic importance for the development of stable cell lines.
Show more [+] Less [-]Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling
2018
Kazuń Barbara | Małaczewska Joanna | Kazuń Krzysztof | Żylińska-Urban Joanna | Siwicki Andrzej K.
Introduction: Immune-potentiating functions of Lactobacillus plantarum strains in the common carp were evaluated.
Show more [+] Less [-]Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite
2018
Samorek-Pieróg Małgorzata | Karamon Jacek | Cencek Tomasz
Taenia solium is a parasite causing porcine cysticercosis and human taeniosis and cysticercosis, parasitic zoonoses with a serious public health and economic influence. It has been globally ranked as the top foodborne parasite by the Food and Agriculture Organisation of the United Nations (FAO) and the World Health Organisation (WHO). This parasite is transmitted mainly in countryside regions where animals are free roaming, having access to human faeces, and infected pork is widely available. More developed countries eliminated cysticercosis; nonetheless, there are insufficient data about the current endemicity status of T. solium, due to increased human migration from endemic areas. Formally submitted statistics on cysticercosis in pigs are extremely inadequate. This is the result of not reporting all cases of the disease by some countries and lack of molecular verification during identification of the parasite. There is a need to develop diagnostic tests with increased sensitivity and specificity. The purpose of the present review is to summarise current knowledge about diagnostic and control methods concerning T. solium infection. The article does not address the diagnostics of human cysticercosis, since there is a distinct medical field which should be discussed separately. The paper focuses mainly on identifying the sources of T. solium infection, presenting the methods to detect and control porcine cysticercosis and taeniosis in humans.
Show more [+] Less [-]Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses
2018
Liu Ya-Li | Ding Yao-Zhong | Dai Jun-Fei | Ma Bing | He Ji-Jun | Ma Wei-Min | Lv Jian-Liang | Ma Xiao-Yuan | Ou Yun-Wen | Wang Jun | Liu Yong-Sheng | Chang Hui-Yun | Wang Yong-Lu | Zhang Qiang | Liu Xiang-Tao | Zhang Yong-Guang | Zhang Jie
Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.
Show more [+] Less [-]Ameliorative effects of Helianthus annuus against nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycaemia in albino rats
2018
Onoja Samuel Okwudili | Udem Samuel Chukwuneke | Anaga Aruh Ottah
The study evaluated the ameliorative effects of Helianthus annuus leaf extract on nephrotoxicity, cardiac, and haematologic disorders in alloxan-induced hyperglycaemic rats.
Show more [+] Less [-]Avian poxvirus infection in Polish great tits (Parus major)
2018
Kozdruń Wojciech | Styś-Fijoł Natalia | Czekaj Hanna | Indykiewicz Piotr | Sandecki Rafał | Niczyporuk Jowita Samanta
Introduction: Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (Parus major) with the avian poxvirus in Poland.
Show more [+] Less [-]Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples
2018
Kędrak-Jabłońska Agnieszka | Budniak Sylwia | Szczawińska Anna | Reksa Monika | Krupa Marek | Szulowski Krzysztof
Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2.
Show more [+] Less [-]GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum
2018
Taşçı Serap Koral | Bingöl Seyit Ali
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.
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