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Protective effects of bisoprolol against cadmium-induced myocardial toxicity through inhibition of oxidative stress and NF-κΒ signalling in rats Full text
2021
Liu Jinhua | Xie Ying | Han Zhujun | Wang Hailong | Xu Wenhu
Protective effects of bisoprolol against cadmium-induced myocardial toxicity through inhibition of oxidative stress and NF-κΒ signalling in rats Full text
2021
Liu Jinhua | Xie Ying | Han Zhujun | Wang Hailong | Xu Wenhu
The aim of the study was to investigate the mitigative effects of bisoprolol (BIS) in cadmium-induced myocardial toxicity on oxidative stress and its inhibitive effect on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signalling in rats.
Show more [+] Less [-]Protective effects of bisoprolol against cadmium-induced myocardial toxicity through inhibition of oxidative stress and NF-κΒ signalling in rats Full text
2021
Liu, Jinhua | Xie, Ying | Han, Zhujun | Wang, Hailong | Xu, Wenhu
The aim of the study was to investigate the mitigative effects of bisoprolol (BIS) in cadmium-induced myocardial toxicity on oxidative stress and its inhibitive effect on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signalling in rats. Male albino Wistar rats were assigned to control, Cd, BIS 2 (2 mg/kg b.w.) and BIS 8 (8 mg/kg b.w.) groups with nine rats in each. Over four weeks, the control group was administered 1% gum acacia, all other groups received 3mg/kg b.w. CdCl₂ dissolved in distilled water, and the BIS groups were additionally given bisoprolol in gum acacia. Blood samples were collected for biochemical estimations. Blood pressure and serum biomarker (lactate dehydrogenase, aspirate transaminase, alanine transferase and creatine kinase-MB, enzyme (superoxide dismutase, lipid hydroxy peroxidase, catalase and malondialdehyde), and tumour necrosis factor alpha (TNF-α) concentrations were measured. Western blot analysis was conducted for NF-κB and glutathione S-transferase (GST). After sacrificing the rats, cardiac tissue samples were examined histopathologically. Our findings pointed to a significant decrease (P < 0.05) in the studied serum biomarkers and levels of the relevant enzymes in the BIS 8 group compared to the Cd group. A significant decrease (P < 0.05) in NF-kB p65 expression and TNF-α levels was noted in the BIS 8 group relative to the BIS 2 and Cd groups, indicating a reduction at a higher dose. In microscopy, histopathological changes in the cardiac muscles of the BIS 8 group were evident compared to those of the Cd group. BIS seemed to have protective effects against cardiac injury induced by cadmium and could be considered a novel therapeutic drug and prognostic biomarker in the pathology of the many cardiovascular diseases caused by heavy metal intake.
Show more [+] Less [-]The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice Full text
2021
Lu Nihong | Yang Yongrui | Li Xiaofei | Li Jie | Cheng Jie | Lv Zhengxuan | Du Yingrong
The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice Full text
2021
Lu Nihong | Yang Yongrui | Li Xiaofei | Li Jie | Cheng Jie | Lv Zhengxuan | Du Yingrong
Piperlongumine (PL) is a bioactive alkaloid and medicinal compound of piperamide isolated from the long pepper (Piper longum Linn). It has demonstrated bactericidal action against Mycobacterium tuberculosis (MTB), the cause of pulmonary tuberculosis; nevertheless, immunomodulatory activity had not been identified for it in MTB-triggered granulomatous inflammation. This study investigated if piperlongumine could inhibit such inflammation.
Show more [+] Less [-]The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice Full text
2021
Lu, Nihong | Yang, Yongrui | Li, Xiaofei | Li, Jie | Cheng, Jie | Lv, Zhengxuan | Du, Yingrong
Piperlongumine (PL) is a bioactive alkaloid and medicinal compound of piperamide isolated from the long pepper (Piper longum Linn). It has demonstrated bactericidal action against Mycobacterium tuberculosis (MTB), the cause of pulmonary tuberculosis; nevertheless, immunomodulatory activity had not been identified for it in MTB-triggered granulomatous inflammation. This study investigated if piperlongumine could inhibit such inflammation. Mycobacterium tuberculosis strain H37Rv was subjected to a broth microdilution assay. Piperlongumine at 5, 15, and 25 μg/mL, 0.2% dimethyl sulphoxide as control or 4 μM of dexamethasone were tested in vitro on MH-S murine alveolar macrophages. BALB/c mice were orally administered PL at 50, 100 and 150 mg/kg b.w. after trehalose-6,6-dimycolate (TDM) stimulation. Chemokine and cytokine concentrations were determined in lung supernatants. Flow cytometry and Western blot analysis were performed to determine phosphorylated spleen tyrosine kinase (Syk), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways. Piperlongumine inhibited inflammatory mediators and adherence of lymphocyte function-associated antigen 1 to MH-S cells following TDM activation. It also improved macrophage clearance of MTB. In TDM-stimulated MH-S cells, PL significantly influenced the macrophage inducible Ca²⁺-dependent lectin receptor (Mincle)-Syk-ERK signalling pathway. Oral dosing of PL effectively suppressed the development of pulmonary granulomas and inflammatory reactions in the TDM-elicited mouse granuloma model. PL as an inhibitor of MTB-triggered granulomatous inflammation may be an effective complementary treatment for mycobacterial infection.
Show more [+] Less [-]Occurrence and antimicrobial resistance of enterococci isolated from goat’s milk Full text
2021
Gołaś-Prądzyńska Marlena | Rola Jolanta G.
Occurrence and antimicrobial resistance of enterococci isolated from goat’s milk Full text
2021
Gołaś-Prądzyńska Marlena | Rola Jolanta G.
Enterococci are widespread, being part of the bacterial flora of humans and animals. The food chain can be therefore considered as the main route of transmission of antibiotic resistant bacteria between the animal and human populations. Milk in particular represents a source from which resistant bacteria can enter the human food chain. The aim of the study was to determine the occurrence and resistance to antimicrobial agents of Enterococcus spp. strains isolated from raw goat’s milk samples.
Show more [+] Less [-]Occurrence and antimicrobial resistance of enterococci isolated from goat’s milk Full text
2021
Gołaś-Prądzyńska, Marlena | Rola, Jolanta G.
Enterococci are widespread, being part of the bacterial flora of humans and animals. The food chain can be therefore considered as the main route of transmission of antibiotic resistant bacteria between the animal and human populations. Milk in particular represents a source from which resistant bacteria can enter the human food chain. The aim of the study was to determine the occurrence and resistance to antimicrobial agents of Enterococcus spp. strains isolated from raw goat’s milk samples. A total of 207 goat’s milk samples were collected. Samples were cultivated on selective media and confirmed as E. faecium or E. faecalis and screened for selected resistance genes by PCR. Drug susceptibility determination was performed by microdilution on Sensititre EU Surveillance Enterococcus EUVENC Antimicrobial Susceptibility Testing (AST) Plates and Sensititre US National Antimicrobial Resistance Monitoring System Gram Positive CMV3AGPF AST Plates. Enterococcal strains totalling 196 were isolated, of which 40.8% were E. faecalis and 15.3% were E. faecium. All tested isolates were susceptible to linezolid, penicillin and tigecycline. For most other antimicrobials the prevalence of resistance was 0.5–6.6% while high prevalence of quinupristin/dalfopristin (51.5%), tetracycline (30%) and lincomycin (52%) resistance was observed. This study affords better knowledge concerning the safety of raw goat’s milk in terms of the enterococci possible to isolate from this foodstuff. It seems that enterococci in milk are still mostly susceptible to antimicrobials of major concern as multiply resisted drugs, such as gentamycin and vancomycin. However, the presence of multi-resistant strains in goat milk is cause for apprehension.
Show more [+] Less [-]Efficacy of Amoxicillin-Clavulanic Acid, Ceftiofur Sodium and Florphenicol in Purebred Arabian Mares Susceptible to Post-Breeding Endometritis Full text
2021
Atasever, Merve | Emre, Birten
Post-breeding endometritis is among the most important causes of infertility in purebred Arabian mares. In this study, it was aimed to evaluate the diagnosis of post-breeding endometritis by various intrauterine antibiotics administrations and to determine the most successful treatment protocol in terms of achieving pregnancy. For this purpose, a total of 80 purebred Arabian mares, that had no reproductive problems in the previous season but were susceptible to post-breeding endometritis in the season of the study were included. During the ovulation follow up performed 24 and 48 hours after the insemination, mares with an intrauterine fluid accumulation larger than 2 cm in diameter were considered susceptible to post-breeding endometritis. These mares were divided into 3 different intrauterine treatment groups: Group I (Amoxicillin-Clavulanic Acid, n=20), Group II (Ceftiofur Sodium, n=20) and Group III (Florfenicol, n=20). The mares in the control group were not given any treatment other than placebo (0.09% NaCl) (Group IV, n=20). Oxytocin (IM) was administered to all mares in the treatment and control groups 2-3 hours after the application, in 3 doses at 6 hour intervals. An examination was performed at the 24th and 48th hours following the ovulation to determine ovulation. Pregnancy examinations were carried out by ultrasound on the 15th, 30th and 45th days after the ovulation. As a result of the treatments, pregnancy rates among the groups were found as 40%, 60%, 55% and 20%, respectively. As a result, it was concluded that Ceftiofur sodium and Florfenicol applications are effective (p<0.05) in terms of pregnancy rates in mares susceptible to post-breeding endometritis.
Show more [+] Less [-]Effects of Age and Gender on The Concentrations of Plasma Homocysteine, Vitamin B12 and Folic Acid in Angora Cats Full text
2021
Kabakci, Ruhi | Bülbül, Selda Fatma
Homocysteine (Hcy) is an amino acid containing sulfur group in methionine metabolism regulating by vitamin B12 and folic acid. In recent years, it has been used as a biomarker for the diagnosis of several diseases. Therefore, in this study, it was aimed to investigate normal values of plasma Hcy, vitamin B12 and folic acid in Angora cats considering the age and gender. Thirty-two blood samples were collected from healthy Angora cats. They were classified as young (5.8±2.6 months, n=16) and adults (76.6±52.6 months, n=16) according to age, and as male (n=16) and female (n=16) according to gender as well. The findings of this study showed that plasma Hcy, vitamin B12 and folate concentrations of all cats were totally 29.94±3.20 nmol/mL, 1179.25±156.96 pg/mL, 28.10±1.50 ng/mL, respectively. These parameters were not differed statistically according to gender (P>0.05). On the other hand, it was observed that plasma concentrations of Hcy, and vitamin B12 were significantly (P<0.05) increased by ageing, except folate levels. In conclusion, this is the first study represents the normal values of plasma Hcy, vitamin B12 and folic acid in Angora cats considering age and gender It is supposed that findings of this study may offer scientific data for researchers, veterinarians, or further comprehensive studies.
Show more [+] Less [-]Trace Element Levels in Naturally Infected Dogs with Giardiasis Full text
2021
Ural, Kerem | Erdogan, Songul | Tarhan, Duygu | Erdogan, Hasan | Ayan, Adnan | Bayrakal, Alper | Parkan Yaramıs, Cagla | Ercan, Alev Meltem | Or, Erman
As a well-known protozoan zoonotic giardiasis has manifested itself with various clinical findings in mammal species including human and dogs. Absorption of some essential biochemical elements required to growth has interrupted in giardiasis being tight relationship with malnutrition. Thus, the aim of the present study was to determine the serum trace element levels in dogs infected with giardiasis. For this purpose, totally 16 dogs enrolled in the study and 8 of these had diarrhoea compatible with giardia (infected group) and others were grouped into healthy. Diagnosis of giardia was performed by microscopical evaluation of cysts and supported by rapid test from faecal samples. In the infected group, copper (Cu), magnesium (Mg) and zinc (Zn) levels had significant differences to healthy control. Giardia infected dogs had a significant increase in Cu and significantly reduces in Mg and Zn levels. It was thought that unclear mechanism of decreased Zn levels might be related to elevated Cu involving enzyme systems. And also, malabsorption and diarrhoea were proposed mechanism for altered Mg levels, which could counteract by blocking of intestinal absorption. Consequently, Cu, Mg and Zn levels were affected in giardiasis and the addable of these elements to the treatment should be revealed by further studies.
Show more [+] Less [-]Determination of Tear Volume and Intraocular Pressure in Saanen Goat and Sakiz Sheep in Similar Environmental Conditions Full text
2021
Kibar Kurt, Büşra | Bulut, Osman | Bozkan, Zeynep | Bilgen Şen, Zeynep | Belge, Ali
The aim of this study is to identify physiological reference values for Schirmer tear test (STT) and intraocular pressure (IOP) results, in healthy sheep and goats. The study included 56 adult Sakiz sheep and 38 Saanen goats with no ocular disease. Tear volume was measured by a sterile STT strip. IOP was measured with Tono-Vet. IOP and STT readings were carried out in July, at 12.30-14.30 hrs. Measurements were write down in similar conditions in sheep and goats (at 41.8°C-30% humidity and 40.5°C and 32% humidity, respectively). No statistically significant difference was determined between the mean IOP and STT values of the left eye and the right eye in the statistical analysis of goats and sheep (P>0.05). The mean STT values were 10.6±3.03 mm/min in Saanen goats and 11.8±3.35 mm/min in Sakiz sheep. The mean IOP values were 16.5±5.46 mmHg and 17.7±4.57 mmHg for Saanen goats and Sakiz sheep, respectively. A statistically significant difference was determined in the STT (P<0.05) and IOP (P<0.01) values between goats and sheep. In the literature review for reference IOP and STT levels, there is no report for Sakız sheep. Reference values of STT and IOP in summer conditions in Saanen goats and Sakiz sheep were revealed and provide more information for experimental and clinical studies.
Show more [+] Less [-]Direct Detection of Cryptosporidium spp. In Cattle In Karbala Province and its Environs, Iraq Full text
2021
Alali, Firas | Jawad, Marwa | kh.k.Al-khayat, Kadhim
Gastrointestinal diseases are caused by many of the apicomplexan protozoan parasites and cause significant clinical diseases. Cryptosporidiosis is one of the most important diseases of young ruminant livestock, particularly neonatal calves. This study investigates the prevalence of Cryptosporidium infection in Karbala farm cattle. Totally, 1360 fecal samples were collected from cows and calves between (1 week to 5years old) from both genders, from different regions of Karbala. Different fecal examination methods (direct fecal smear, fecal flotation with saturated salt solution and faecal smear stained by modified Ziehl-Neelsen were performed to determine Cryptosporidium spp. oocysts and other mixed parasites in the fecal samples. The results showed that 29.4% of the fecal samples were detected positive for cryptosporidiosis (400/1360). The highest infection rate appeared (41.9%) in the age group from 3-6 months old, and less rate was (15.1%) in 2 years (P≤0.05). Additionally, there was no significant variation found in infection rates between males (33.8%) and females (25.6%). Mixed infections have been reported with Eimeria spp. oocysts (7.3%), Giardia lamblia cysts (1.4%) and Trichostrongylus spp. eggs (5.1%) (P≤0.05). The results indicated the requirement for the eradication of the gastrointestinal parasites by deworming and a good management system in the sampled region.
Show more [+] Less [-]Alterations in Gut Integrity Due to Heat Stress Among Dairy Cattle of Aydin City: Analytical Interpretation of Zonulin Levels Within Repetitive Measurements Full text
2021
Alıc Ural, Deniz | Ural, Kerem | Erdogan, Hasan | Erdogan, Songul
Animal health and production are impaired with heat stress inducing dysfunction of intestinal barrier. In this study, it was aimed to determine that the effect of heat stress on gut health via circulating zonulin concentrations. Holstein dairy cows (n=7, age of 2-5 years) were housed in Aydin municipality with higher temperatures in summer. Blood sample for serum zonulin levels were collected at 12 pm and 00 am with temperature records of 44°C and 31°C respectively. Serum zonulin levels were found to be statistically significant (p=0,012) at night in contrast to daylight analytes. With this study it was appeared that heat stress had a negatively impact on intestinal integrity among cows.
Show more [+] Less [-]Problems Encountered by Clinician Female Veterinarians and Their Ethical Approaches in Turkey (II) Full text
2021
Koc, Aysun | Ayvazoglu Demir, Pınar
Bu çalışmada, klinik yöneticisi kadın veteriner hekimlerin cinsiyete dayalı olarak karşılaştıkları zorluk ve avantajların ortaya çıkarılması ve deontolojik / etik yaklaşımlarının belirlenmesi amaçlanmıştır. Yapılan anket sonuçlarında, kadın veteriner hekimlerin% 90,8'inin işini tatmin ederek yaptığı; Katılımcıların% 33,8'i kadın veteriner olmanın avantajlı yönleri olduğunu düşünürken, dezavantajlarının daha fazla olduğunu düşünenlerin oranı% 66,2 olarak tespit edilmiştir. Kadın veteriner hekimlerle yapılan görüşmelerde katılımcıların% 58,2'sinin veteriner istihdamında seçimin tamamen erkekler lehine yapıldığını düşündüğü,% 18,2'sinin ise kısmi olduğu belirtilmiştir. Ayrıca meslektaşların uygulama sırasında etik ve deontolojik ilkelere uygun davrandıkları tespit edilmiştir. tamamen katılımcıların% 47,3'üne ve kısmen katılımcıların% 28,2'sine göre. Sonuç olarak, çeşitli mesleklerden kadın işçilerin karşılaştıkları sorunlar arasında benzerliklerin tespit edilmesine katkı sağlamayı amaçlayan bu araştırma, yönetici sahibi kadın veteriner hekimlerin cinsiyete dayalı bir yaklaşımla farklı oranlarda karşı karşıya olduğunu tespit etmektedir.
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