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Dose Dependent Cytotoxicity Effect of Doxorubicin on Breast Cancer Cell Line (AMJ13) Proliferation: in Vitro Study
2023
Eman H. Hamed | Siham A. Wadi
The purpose of the current investigation was to identify the dose-dependent effect of doxorubicin on the proliferation of the AMJ13 cell line. The AMJ13 breast cancer cell line was used to investigate the cytotoxicity of the medication doxorubicin. The median inhibitory concentration (IC50) was calculated using the Methyl thiazolyltetrazolium (MTT) assay. Doxorubicin's IC50 value, which ranged from 162.2 to 308,3, was 223.6. Doxorubicin inhibited the proliferation of AMJ13 cells to a greater or lesser extent at concentrations of 1000, 500, 250, 125, 62.5, and 31.2 g/ml (58.8%, 46.4%, 32.3%, 23.8%, 11.3%, and 0.896%). respectively. The percentage of cytotoxicity (CT) After 72 hours of treatment, doxorubicin inhibited MCF7 cell growth in a dose-dependent manner, with a CT% of 90% at a dosage of 50 M. To sum up, doxorubicin displays strong cytotoxicity against the AMJ13 breast cancer cell line. It could be concluded that the effect of doxorubicin on the proliferation of theAMJ13 is dose dependent. In addition, morphological changes and apoptosis significantly enhance the inhibition of growth.
Show more [+] Less [-]Epidemiological Investigation of Helicobacter pylori as an Emerging Zoonosis in Matrouh Province, North-West Egypt: A Community Based Cross-sectional Study
2023
Ibrahim M. Rabah | Mohamed A. Nossair | Elsayed E. Hafez | Mohamed M. Elkamshishi | Eman E. Khalifa
Helicobacter pylori is newly emerging bacteria and one of the most common infections worldwide with over one-half of the world is infected with this organism. The aim of this study was to determine the prevalence of H. pylori among sheep, camels, and humans in Matrouh Province, North-West Egypt using H. pylori stool antigen enzyme immunoassay and stool PCR. A total of 250 stool samples were collected from farm animals (sheep and Camels) and humans in Matrouh Province. Samples were examined using H. pylori Stool Antigen Enzyme Immunoassay test and H. pylori 16S rRNA PCR. Statistical analysis was applied using Chi2 and IBM SPSS Statistics 25. The overall prevalence of H. pylori infection in the study by HpSA and PCR were 27.6% and 24.4%, respectively. Based on the results of HpSA test, it was found that the prevalence was 12% and 26% in sheep and camels, respectively, with statistically significant association between the prevalence and locality or age of sheep. Moreover, the prevalence of H. pylori infection in human was 44% by HpSA test with statistically significant association between the prevalence and gender or locality being higher in males than females with greater rural prevalence than urban. On the other hand, there was a non-statistically significant differences between H. pylori prevalence and sex, breed, and health status of examined animals or age, residence, and occupation of enrolled individuals. Conclusively, H. pylori was detected in both animal and human samples is alarming in Matrouh Province. Therefore, there was an urgent need for implementing a proper control program.
Show more [+] Less [-]Lipid Profile and Obese Related Genes of Rats, Potential Therapeutic Effects of Peganum Harmala /Zinc Oxide Nanoparticles
2023
Mervat Abdelsattar | Youssef Elseady | Walaa F. Awadin | Ahmed Hendawy | Abd EL-Aziz A. Diab
The present study investigated the effects of methanolic extract of harmala nanoparticle (H/ZnONP) on the changes in serum lipid profiles, serum leptin levels and liver and kidney PPAR gene expression in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD for one month to half numbers of rats. Non-obese group had three subgroups; G1: Control fed basal diets, G2: basal diets plus H/ZnONPs (200 mg/kg/day) and G3: basal diets plus H/ZnONPs (400 mg/kg/day). However, obese group was divided into three subgroups G4: HFD, G5: HFD+H/ZnONPs (200 mg/kg/day) and G6: HFD+ H/ZnONPs (400 mg/kg/day). After 4 weeks of treatment blood and tissue samples were collected and subjected to laboratory assay of lipid profile and leptin level in addition to expression of PPAR gene in liver and kidney. We also investigated the possible protective effect of H/ZnONP 200 and 400 mg/kg BW on liver and kidney histology. The obtained results demonstrated that H/ZnONP treatment to obese rats significantly decreased serum cholesterols, triglyceride, and LDL level while relative expression of PPAR gene in liver and kidney was significantly increased. Based on the obtained results we can concluded that H/ZnONP supplementation has a potent anti-obesity effect in rats by improving lipid profile, enhance expression of PPAR gene in liver and kidney and improve liver and kidney histology particularly the high dose.
Show more [+] Less [-]Effect of FSH Stimulation with Pluronic® F127 on the Quality of Oocytes Collected by Follicular Aspiration in Bos taurus indicus Heifers
2023
Héctor Javier Narváez | Deicy Villalba Rey | Ricardo Lopes Dias da Costa
In bovine in vitro embryo production (IVP), FSH has been used to improve the quality and developmental capacity of oocytes to reach the blastocyst stage. The objective of this study was to evaluate in Bos taurus indicus heifers the effect of FSH incorporated in Pluronic® F127 on the quality of oocytes collected by follicular aspiration. A total of 30 cyclic Brahman heifers were selected and assigned into one of three experimental groups (control group, FSH-SC and FSH-PF127). Heifers in the FSH-SC group received a single dose of 50 mg FSH subcutaneously and heifers in the FSH-PF127 group were treated with 50 mg FSH incorporated in Pluronic® F127. The rate of viable oocytes was higher for the FSH-PF127 group compared to the control and FSH-SC groups. The administration of a single dose of 50 mg of FSH in Pluronic® F127 influenced the oocyte quality and the rate of viable oocytes of Brahman breed heifers.
Show more [+] Less [-]Evaluation of Locomotor and Morpho-histological Effect for Platelet Rich Plasma and Silver Nanoparticles on Healing Process of Achilles Tendon (Comparative Experimental Study in Rabbits)
2023
Mohamed H. Elrashidy | Mohamed M.A. Salama | Bahaa Eldeen A. Abedellaah | Fatma Abo Zakaib Ali | Sayed F. El-Hawari
Tissue engineering is a new treatment option provided encouraging results in orthopedic surgery. In the present study, we compare between platelet rich plasma (PRP) and silver nanoparticles (AgNPs) in the healing process of achilles tendon. This comparison depending upon the evaluation of locomotor and morpho-histological parameters for each treatment. The current study was carried out on 45 apparently healthy adult female rabbits. Locomotor evaluation was done through numerical score at intervals of 2, 4, 6 and 8 weeks postoperatively. Morpho-histological evaluation was performed through numerical score at intervals of 2, 3 and 6 weeks postoperatively. Results of the current study revealed that PRP and AgNPs were better than control group regarding to lameness, pain on complete flexion of tarsal joint, adhesion of tendon to the skin and signs of inflammation. However, PRP is better than both control and AgNPs groups in decreasing lameness and adhesion of tendon to the skin. Morpho-histological examination revealed that PRP is better than both control and AgNPs in improving color of the tendon. PRP and AgNPs were better than control group concerning collagen maturation, fibroblast number, angiogenesis, and surgical gap reduction. However, PRP is better than both control and AgNPs in decreasing signs of inflammation and increasing collagen maturation. We concluded that using PRP for treatment of ruptured achilles tendon provides better prognosis than AgNPs treatment.
Show more [+] Less [-]Unilateral Uterine Torsion in a Pregnant Cat
2023
Eslam Ahmed Abdullah Hendy | Bardees Khaled Elgohary
A 7-year-old female Shirazi cat was referred by a private clinic to the Department clinic of Veterinary Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University with a history of lethargy, anorexia and confirmed pregnancy by ultrasonography. Clinical examination of the case showed acute abdominal pain and abdominal distention with a bloody vaginal discharge. The radiographic image revealed a tubular soft tissue opacity. Upon stabilization of the case surgical approach by exploratory laparotomy to observe a 90o degree twisted left horn, congested blood vessels and dark red, soft, edematous uterine horn. ovariohysterectomy was performed. Fluid therapy and antibiotics were administered, the case survived and regained appetite a few days post-operative with no need for blood transfusion.
Show more [+] Less [-]Newcastle Disease Virus as Virotherapy Agent Targeting p53 in Rat Fibrosarcoma Models
2023
I Putu Krisna Ardhia Pradnyandika | I Nyoman Mantik Astawa | Anak Agung Ayu Mirah Adi
Repairing wild-type p53 or destroying of mutant p53 is one of the therapeutic targets in cancer. Newcastle disease virus (NDV) is a natural oncolytic virus that has potential as a virotherapy agent in cancer. This virus has been shown to induce cancer cell death. The aim of this study was to determine the expression of p53 in cytoplasm and nucleus of cancer cells and its correlation to the grade of cancer malignancy after NDV therapy in rat fibrosarcoma model. Rat fibrosarcoma model were divided into two groups, i.e., the control group (P0) and the treatment group (P1), each consist of 3 rats. The control group (P0) was injected with 0.5 mL phosphate buffered saline and treatment group (P1) was injected with 0.5 mL NDV Tabanan-1/ARP/2017 intratumorally once a day for four consecutive days. At the end of the study, 15 days post-treatment, all rats were euthanized and fibrosarcoma tissue was collected. Fibrosarcoma tissue was examined using immunohistochemistry to determine p53 expression and histopathological examination with hematoxylin-eosin staining to determine the grade of malignancy. The results of this study, the mutant p53 were more expressed in the control group (P0) than the treatment group (P1). It showed that NDV was significant (P<0.05) to the decrease of mutant p53 expression and positively correlated (P<0.05) to the cancer malignancy in rat fibrosarcoma model. In conclusion, NDV has potential as a virotherapy agent targeting mutant p53 in rat fibrosarcoma models.
Show more [+] Less [-]Transrectal Doppler Ultrasound to Study the Uterine Blood Flow Changes During the Puerperium in the Egyptian Buffaloes
2023
Mohamed Gohar | Samy Zaabel | Wael Eldomany | Asharaf Eldosouky | Walid Tawfik | Heba Sharawy | Mohammed Elmetwally
The aim of the current study was to determine the changes in uterine blood flow as well as uterine biometry during the first 5 weeks after parturition in Egyptian buffaloes. The transrectal noninvasive color Doppler ultrasound and uterine biometry were measured at different time points -7, 0 (day of parturition), 7, 14, 21, 28, 35 after the postpartum period. The mean diameter of the uterine horn decreased significantly from day 0 to day 35 after parturition (P < 0.0001). The uterine involution was completed on day 28, as demonstrated by transrectal palpation and B-mode sonography. Peak systolic velocity (PSV), timed average maximum velocity (TAMV), blood flow volume (BFV), pulsatility index (PI), resistance index (RI), systolic /diastolic (S/D) as well as the diameter of uterine arteries were the Doppler indices for determination of changes in uterine artery blood flow during different time points after parturition. BFV of uterine arteries decreased linearly during the postpartum period in Egyptian buffaloes. The BFV decreased from 3029.21 ml/min (-7d) to 343.84 ml/min (35 d pp) and moderately (P < 0.01) to 731 ml/min on day 28. The TAMV and PSV showed fluctuation in changes during different time points postpartum (P < 0.05). But PI, RI, and SD showed significant increases during the different time points postpartum (P < 0.01). Altogether, the results show that transrectal color Doppler ultrasound is a successful tool for examining uterine changes during the first 5 weeks after parturition in Egyptian buffaloes. The robust changes in uterine blood flow were demonstrated during the first week of the puerperium, The PI, RI, and S/D were also suitable to investigate alterations in uterine perfusion during the next 8 weeks after parturition.
Show more [+] Less [-]Antimicrobial Activity of Starch-based Biodegradable Antimicrobial Films Incorporated with Biosynthesized Silver Nanoparticles Against Multiple Drug-resistant Staphylococcus aureus Food Isolates
2023
Omnia A.M. Ahmed | Fathy Khalafalla | Fatma H.M. Ali | Abdelrahim H.A. Hassan
This study was conducted to determine the antimicrobial activity of starch-based biodegradable antimicrobial films incorporated with biosynthesized silver nanoparticles (Ag-NPs) against multiple drug-resistant Staphylococcus aureus food isolates. Herein, the in-vitro antimicrobial activities of Origanum marjorana (OM) leaf extract, OM essential oil, OM nano-emulsion, chemically synthesized Ag-NPs (chem-Ag-NPs), and OM-based biosynthesized Ag-NPs (bio-Ag-NPs) using OM extracts were determined against a cocktail of three pathogenic Staphylococcus (S.) aureus strains isolated from meat products, using the agar well diffusion assay (AWDA). Afterward, homemade starch-based biodegradable antimicrobial films (SBAF) were incorporated with the suitable antimicrobials, based on AWDA and preliminary experiments, and investigated for their antimicrobial properties against S. aureus cocktail through the disc diffusion assay (DDA). The obtained results showed that in WDA, bio-Ag-NPs (1mM) had a significantly higher antimicrobial activity than chem-Ag-NPs (1mM), with inhibition zones accounting for 23 and 19mm, respectively. Whereas both types of nanoparticles were significantly more potent in their antimicrobial properties than different concentrations of OM extract, essential oil, and nano-emulsion (p<0.05). In concern to SBAF incorporated with antimicrobials, SBAF incorporated with chem-Ag-NPs (SBAF/chem-Ag-NPs) showed a significantly stronger antimicrobial effect than SBAF incorporated with bio-Ag-NPs (SBAF/bio-Ag-NPs) in the DDA, while both types of films produced significantly larger zones of inhibition than other antimicrobials (p<0.05). These homemade biodegradable films incorporated with bio-Ag-NPs could be a good alternative to petroleum-based packaging (plastic) in food packaging applications and meanwhile improve food safety and quality. Further studies investigating the effectiveness of these films on bacterial isolates inoculated in real food samples are suggested.
Show more [+] Less [-]Influence of Long and Short-term Progesterone Administration on Estrous Synchronization and Reproductive Performance in Ewes During May Season
2023
Wael Eldomany | Abdel-Khalek Abdel-Khalek | Wael Khalil | Ahmed Yousif | Bedir El-Saidy | Mervat Hassan
Estrous synchronization is a promising management device to get better reproductive proficiency, particularly in ruminants. However, limited information is available about the influence of long and short-term progesterone administration on estrous synchronization and reproductive performance in ewes during May season, particularly in Egypt. In this study, a total of 144 ewes have been utilized for estrous synchronization and reproductive performance in ewes during May season. Ewes were separated into six groups with different treatments as follows: G1, control (natural mating), G2, ewes were treated with 20 mg Cronolone vaginal impregnated sponges (Flurgestone acetate, FGA). The sponge was remained intravaginal for 11 days. Each ewe was intramuscularly (i.m.) injected with 500 international unit (IU) Pregnant mare serum gonadotrophin (PMSG) on the day of sponge withdrawal (day 11), G3, ewes were treated with intra-vaginal sponges. The sponge was remained intra-vaginal for 11 days, on day 10, each ewe was injected with 25 mg ultra-short progesterone (P4) (1 ml Lutone). Each ewe was injected i.m. with PMSG (500 IU) on day 11. G4 ewes were treated with intra-vaginal sponges. The sponge was remained for 11 days. Teaser ram was introduced to treated ewes after sponge withdrawal. The 5th group (G5), ewes were treated with intra-vaginal sponges. The sponge was remained intravaginal for 11 days. On day 10, each ewe was injected with 25 mg P4 (1 ml Lutone). Teaser ram was introduced to treated ewes after sponge withdrawal. The 6th group (G6), the same treatment of G2, but the sponge was inserted intra-vaginal only for 6 days. Each ewe was i.m. injected with 500 IU pregnant mare's serum gonadotropin (PMSG) on day 6. Synchronization was better in G2, G3 and G6, respectively and all short-term group animals came into estrus. In addition, onset of estrus and its duration differs significantly among groups. Reproductive performance also showed a significant difference among groups and the short-term group has the highest fertility rate. Given the above information, our data concluded that progestagens synchronized estrus (long term or short term) and administration of PMSG (500 IU) at sponge withdrawal improves the reproductive efficiency of artificially inseminated crossbred ewes.
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