Bacterial infection followed by implant fixation is a common complication after surgery. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity of magnetron sputtered silver nanoparticles was studied. Sputtering was done using radiofrequency magnetron sputtering technology. Film applicator coating assay was used to assess the antibacterial effect of the coated titanium implants. SEM examination revealed successful deposition of silver nanoparticles on the titanium surface. The average diameter of the nanoparticles was 40-60 nm. SEM examination before incubation and after incubation of bacteria was done. The bactericidal ratio between the uncoated and coated implant was determined.

Bacteriological quality of khoa based sweets marketed through different retail outlets of Thrissur city was evaluated based on total viable count, coliform count, yeast & mould count and staphylococcal count. Altogether 72 samples of khoa based sweets such as peda, burfi and gulabjamun were evaluated for their bacteriological quality. Out of the total peda samples tested, coliforms were prevalent in 37.5%, yeast and mould in 91.7% and staphylococcal organisms in 41.7% of the samples. In burfi, coliforms were prevalent in 66.6%, yeast and mold in 95.8% and staphylococcal organisms in 83.3 % of the samples tested. In gulabjamun, coliforms, yeast and mould and staphylococcal organisms were present in 45.8%, 91.6% and 70.8% respectively of the samples tested. The highest rate of contamination was recorded in burfi while the lowest was recorded in peda. Strict hygienic measures during preparation and storage are recommended to ensure the quality of the products. 

Nanotechnology has been used in a variety of medical applications, including diagnostics, immunisation, medication and gene delivery. Nanotechnology has opened up new ways for molecular biology and biotechnology applications, and it has revolutionised practically every area of veterinary and animal sciences by introducing new, small-scale instruments and materials that are advantageous to living species. Metallic nanoparticles, quantum dots, carbon nanotubes, magnetic nanoparticles, fullerenes,  liposomes, and dendrimers are among the several forms of nanomaterials employed in detection of various diseases, therapy, drug administration, animal nutrition and breeding. It is concluded that, before making nano-products available for animal use, extensive risk evaluations should be carried out to ensure their safety. So, this article provides an overview of existing and promising nano applications in veterinary research that may lead to increased efficiency and production.

Oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage in a variety of liver disorders. Hence, there is a great demand for the development of agents with potent antioxidant effect. The aim of the present investigation is to evaluate the efficacy of raw and nanoforms of resveratrol and silibinin as a hepatoprotective and an antioxidant against diethylnitrosamine induced hepatocellular damage. 0.01% diethylnitrosamine administration to rats resulted in significantly elevated levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GSH) and total protein (TP) in the liver tissue. Post-treatment with the nano resveratrol (50 mg/kg), nano silibinin (50 mg/kg), raw resveratrol (50 mg/kg), raw silibinin (50 mg/kg) orally for 30 days significantly reversed the diethylnitrosamine induced alterations in the liver tissue and offered almost complete protection. The results from the present study indicate that both nano forms exhibit good hepatoprotective and antioxidant potential against diethylnitrosamine induced hepatocellular damage in rats as compared to raw forms.

Nutritional evaluation of different pulse chunnies was carried using Near Infrared Reflectance Spectroscopy (NIRS) and validated with wet analysis for inclusion in livestock feeds. The portable NIRS spectrophotometer FOSS XDS RCA and FOSS 6500 chemometrics software were used for the study. A total of 324 samples of four pulse chunnies (red gram (RGC), green gram (GGC), black gram (BLGC) and bengal gram (BGC)) were assessed using NIRS. It revealed that RGC had significantly (P<0.01) highest DM content than other three samples. The mean CP content was highest (P<0.01) in GGC and lowest in BGC and significantly (P<0.01) higher ether extract (EE) was recorded in BLGC and RGC and lowest in BGC samples. The ash content was significantly (P<0.01) highest in BLGC and lowest in RGC samples. Neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) contents were higher (P<0.01) in BGC and lower in GGC samples. Wet analysis shown, the DM content did not differ (P>0.05) among pulse chunni samples, while significantly (P<0.01) higher organic matter was recorded in RGC and lowest in BLGC samples. The CP was higher (P<0.01) in BLGC and lower in BGC and significantly (P<0.01) highest crude fibre recorded for BGC, RGC and was lowest in BLGC and GGC samples. Highest (P<0.05) EE was observed in BLGC while lowest in BGC. There was no significant (P>0.05) difference in nitrogen free extract among pulse chunnies, while ash content was significantly (P<0.01) higher in BLGC and lower in RGC samples. NDF, ADF and ADL were significantly (P<0.01) higher in BGC and lower in GGC samples. The study concludes that the NIRS technique can be used to screen large number of samples in lesser time for using them in livestock feed formulations as there is a high correlation between NIRS and conventional feed analysis.

Leptospirosis is one of the most important zoonotic disease caused by the pathogenic leptospires Leptospira interrogans. In this study, four pathogenic Leptospira serovars were selected based on the seroprevalence data, their LPS extracted, assessed for its purity and seroreactivity and processed for the determining the fatty acid profiles. The average concentration of the LPS extracted from the cultures across the serovars was 742.75+19.4 µg/ml with the polysaccharides contributing to 77.2 % of the LPS (573.6+18.01 µg/ml). The common fatty acids that were present in all the four serovars included butyric acid (C4:0), capric acid (C10:0), palmitoleic acid (C16:0),  eicosapentaenoic acid (C20:5n3) and docosapentaenoic acid (C22:6n3). The heptadecanoic acid (C17:1) was present in serovars australis and autumnalis; pentadecanoic acid (C15:0) in canicola and hardjo serovars and caproic acid (C6:0) in the serovars autumnalis and canicola. The fatty acid eicosatrinoic acid (C20:3n3) was unique to the serovar autumnalis and the myristic (C13:0) and docosadienoic acid (C22:2) being unique to the serovar hardjo. Future studies to understand the fatty acid profile of the other common pathogenic circulating Leptospira LPS will add scientific evidence to the minor cross-reactivity observed across the serovars in MAT.

A pregnant graded Murrah buffalo at eight months of gestation in third parity having bilateral distension of abdomen was presented. The case was diagnosed as hydramnios and treated with dinoprost tromethamine, diethyl stilbesterol, valethamate bromide and dexamethasone. After 76 hours, cervix was dilated fully and trans-cervical amniocentesis was performed and approximately 60-70 litres of amniotic fluid was drained and a dead male fetus with cleft palate was delivered.

A one year old, non descript doe presented with the history of straining to deliver and hanging of ruptured water bag from the vulva was diagnosed as dystocia. The attempts for manual extraction of foetus were failed and a dead emphysematous male foetus was removed by caesarean section. Hydrocephalus and prognathism were observed in the dead foetus. The doe uneventfully recovered postoperatively.

A pluriparous full-term pregnant buffalo was presented with a history of unproductive straining with improper relaxation of the vulva. Detailed obstetrical examination revealed the presence of two fetuses that were conjoined twins with attachment present at the sternal region of the thorax of the fetuses. The conjoined twins (Ziphopagus monster) were delivered per-vaginal by episiotomy technique.

A postpartum graded Murrah buffalo that calved 25 days before was presented with a history of swelling of the perianal region immediately after parturition. On examination, ventral anal region was found to be enlarged with a pouch that contained around 1.5 kg of dung. The buffalo was given caudal epidural anaesthesia and the perianal mucous membrane was scarified and sutured. Postoperatively antibiotic, anti-inflammatory and anti-histamine were given for 5 days and animal exhibited normal defecation without straining. The correction of unusual case of peri-partum third degree perineal laceration associated with rectal-vaginal fistula and rectal pouch formation in a graded Murrah buffalo, its management and successful recovery is reported.