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Proteins specified by bovine herpesvirus-2.
1988
Adams S.W. | Balachandran N. | Hutt Fletcher L.M.
Fixation procedures for retention of cellular morphologic features and for preservation of immunoreactivity of canine paramyxovirus antigens.
1988
Baumgartner W. | Krakowka S.
Pyrrolizidine alkaloid-induced liver disease in horses: an early diagnosis.
1988
Mendel V.E. | Witt M.R. | Gitchell B.S. | Gribble D.N. | Rogers Q.R. | Segall H.J. | Knight H.D.
Influence of alterations in heart rate on echocardiographic measurements in the dog.
1988
Jacobs G. | Mahjoob K.
Sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood.
1988
Paape M.J. | Miller R.H.
A study was conducted to examine sources of variation introduced into a phagocytosis assay as a result of the isolation of neutrophils from bovine blood, including variation attributable to isolation of neutrophils from blood, variation between duplicate determinations of percentage phagocytosis, and the variation in the ability of neutrophils isolated from blood (over repeated collections from the jugular vein) to phagocytose. For the phagocytosis assay, jugular venous blood from each of 4 cows was divided into 2 equal portions. The neutrophils were isolated by lysis of red blood cells with 0.2% sodium chloride. The neutrophils (2 X 10(7)) were incubated in duplicate with 32P-labeled Staphylococcus aureus ([32P]SA; 2 X 10(8)) inskimmed milk samples (2.5% final concentration) prepared from 4 cows. This process was repeated thrice on neutrophils isolated from 4 cows at 2-week intervals. The proportions of variation in percentage of 32P-labeled S aureus phagocytosed between duplicate neutrophil isolations and between duplicate assay determinations were 0 and 1%. Differences among skimmed milk sources and among runs, using blood neutrophils taken at different times from the same donor cow, accounted for 62 and 36% of the total variation. The results indicated that variation arising from blood neutrophil isolation introduced into a phagocytosis assay within a single-day trial is of no concern. The large variation among skimmed milk sample sources indicated differences among cows in the ability of their milk to support phagocytosis. The variation in neutrophil isolations over time for any cow was considered too large to allow for evaluation of physiologic and environmental effects on phagocytosis of neutrophils isolated from blood.
Show more [+] Less [-]Prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle.
1988
Collins J.K. | Teegarden R.M. | MacVean D.W. | Smith G.H. | Frank G.R. | Salman M.D.
The specificity of serum antibodies for the polypeptides of bovine respiratory syncytial virus (BRSV) was examined, using sera obtained from feedlot and range cattle. Test results in sera from feedlot cattle indicated a 60% rate of seroconversion and 95% seropositivity to BRSV, associated with lack of clinical signs of indicative respiratory tract disease. Exposure to other common respiratory tract viruses also was high (greater than 92% to bovine herpesvirus type 1, bovine viral diarrhea virus, and para-influenza virus type 3). Test results in sera from range cattle indicated BRSV serpositive rates of 28% in calves, 49% in yearling cattle, and 70% in mature cows; clinical signs of respiratory tract disease were not observed in these cattle. Antibodies to BRSV in sera from cattle in both environments reacted predominantly with polypeptides of molecular weight 80,000 through 85,000, 40,000 and 28,000. Reactivity to a glycoprotein of molecular weight between 43,000 and 44,000 and to several glycopolypeptides of smaller molecular weight increased in serum specimens obtained from feedlot cattle between time of entry into the feedlot and slaughter.
Show more [+] Less [-]Fiber type, fiber size, and capillary geometric features of the semitendinosus muscle in three types of dogs.
1988
Rosenblatt J.D. | Kuzon W.M. Jr. | Pynn B.R. | Plyley M.J. | McKee N.H.
The fiber type, fiber size, and capillary geometric features were determined from the center of the proximal half of the left and right semitendinosus muscles in 5 mixed-breed dogs, 5 hound-type dogs, and 5 Beagles. There were no significant differences between the left and right muscles of each dog. Comparisons among the 3 groups of dogs revealed that the hound-type dogs had the largest fibers (type I and type II); however, the 3 groups were similar in their fiber-type percentages and their capillary geometric features.
Show more [+] Less [-]Campylobacter jejuni infections in gnotobiotic pigs.
1988
Boosinger T.R. | Powe T.A.
Pharmacokinetics and bioavailability of ceftriaxone administered intravenously and intramuscularly to calves.
1988
Soback S. | Ziv G.
Partial purification and characterization of bovine fibroblast interferon.
1988
Allen G.K. | Grothaus G.D. | Rosenquist B.D.
Bovine fibroblast interferon (BoF-IFN), produced in primary bovine embryonic kidney cell cultures after priming and infection with bluetongue virus, was purified by controlled pore glass (CPG) chromatography to a specific activity of 10(6) U/mg of protein, with 40% recovery of the original activity. The crude IFN was concentrated more than sevenfold during purification. This proved to be a relatively simple, practical method of obtaining sufficient quantities of partially purified natural BoF-IFN for further studies. The CPG-purified BoF-IFN was further concentrated by sequential ultrafiltration and was analyzed by sodium dodecyl sulfate/polyacrylamide-gel electrophoresis (SDS-PAGE). Interferon, recovered from denaturing conditions either by dialysis against phosphate-buffered saline solution or by dilution in cell culture medium containing 10% fetal bovine serum, migrated as a single stainable protein with molecular weight of 21,000 on analytic SDS-PAGE gels. Recovered IFN activity from preparative SDS-PAGE totalled 8.7% of that applied. Attempts to further purify CPG-purified BoF-IFN by zinc chelate affinity chromatography were unsuccessful.
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