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Investigation of canine parvovirus occurrence in cats with clinical signs of feline panleukopenia in Slovakia – pilot study
2024
Citarová Alexandra | Mojžišová Jana | Petroušková Patrícia | Pelegrinová Andrea | Kostičák Maroš | Korytár L’uboš | Prokeš Marián | Vojtek Boris | Ondrejková Anna | Drážovská Monika
Feline panleukopenia is a contagious viral disease caused by the feline panleukopenia virus (FPV). A closely related pathogen is canine parvovirus (CPV), and amino acid substitutions in this virus allow it to acquire a feline host range. In feline hosts, the disease induced by CPV manifests with similar symptoms to those caused by FPV or milder ones, leading to its underdiagnosis. The aim of this study was to determine the presence of CPV type 2 (CPV-2) in cats with clinical symptoms of panleukopenia and to assess the use of commercial CPV antigen tests for the clinical diagnosis of FPV.
Show more [+] Less [-]Comparison of transfer of different sulphonamides from contaminated beeswax to honey
2024
Mitrowska Kamila | Antczak Maja
No maximum residue limits in honey have been legislated in the EU for antimicrobial substances such as sulphonamides, and they are not permitted, therefore, for treating honey bees unless in a cascade system. Since sulphonamides are used illegally in apiculture to treat foulbrood, their residues can be found in honey and other apiculture products, including beeswax. The study aimed to assess the contamination of honey from beeswax containing residues of 10 sulphonamides (sulphadimethoxine (SDM), sulphadoxine (SDX), sulphamonomethoxine (SMM), sulphamethoxazole (SMX), sulphameter (SMT), sulphamethazine (SMZ), sulphamerazine (SMR), sulphadiazine (SDA), sulphathiazole (STZ) and sulphacetamide (SCA)).
Show more [+] Less [-]Extracellular vesicles in cattle infected with bovine leukaemia virus: isolation and molecular analysis
2024
Szczotka Maria | Wasiak Magdalena | Kuźmak Jacek
Exosomes are nanosized lipid bilayer membranous microvesicles, extracellularly released from a variety of mammalian cells. They mediate intercellular signalling by transporting several types of RNA, lipids and proteins and participate in the intercellular exchange of DNA, RNA, micro RNA, proteins and other components. These microvesicles are present in all body fluids in physiological and pathological conditions and reflect the state of the host organism. The aim of the study was the isolation and molecular determination of exosomes in blood and supernatant fluids of bovine dendritic cell cultures infected with bovine leukaemia virus (BLV).
Show more [+] Less [-]The efficiency of mycotoxin binding by sorbents in the in vitro model using a naturally contaminated animal feed
2024
Żybura Adrianna | Jedziniak Piotr
The productivity of domestic animals and the safety of food products derived from them are jeopardised by mycotoxins in animal feed. To control them, feed additives are used, which limit the absorption of mycotoxins in the gastrointestinal tract of animals by binding to them. The study aimed to evaluate the effectiveness of a new in vitro model in experiments on the binding of mycotoxins from buffers and contaminated feed and to confirm the effect of a single sorbent or mixture in binding them.
Show more [+] Less [-]Molecular characteristics of fowl adenovirus strains detected in broiler chickens on diets without immunostimulant supplements
2024
Niczyporuk Jowita Samanta | Kozdruń Wojciech | Tomczyk Grzegorz | Piekarska Karolina | Barabasz Marcin | Michalski Marcin
Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds’ health and lives and are rising in frequency. The risk of these infections in immunocompromised poultry flocks with developed clinical symptoms was analysed through virus detection in broiler chicks and correlation of cases with the birds’ immune strength.
Show more [+] Less [-]Caesium-137 in the muscles of game animals in 2015-2022 – levels and time trend
2024
Czerski Paweł | Gembal Magdalena | Warenik-Bany Małgorzata
Radioactive caesium-137 occurring in the environment may be taken up by plants and animals and pose a trophic threat to humans. Game animals living in forest ecosystems are very good bioindicators of the level of environmental contamination by ionising radiation. The main species measurably exposed to caesium-137 are the wild boar (Sus scrofa), the roe deer (Capreolus capreolus), and the red deer (Cervus elaphus). The study determined the levels of Cs-137 in muscle samples of these game animals in 2015–2022.
Show more [+] Less [-]Vector-borne diseases imported to Poland between 2021 and 2023
2024
Adaszek Łukasz | Staniec Marta | Dokuzeylül Banu | Pisarek Maria | Skrzypczak Maciej | Żółkiewski Paweł | Rutkowska-Szulczyk Małgorzata | Deneka Łukasz | Or Mehmet Erman | Winiarczyk Stanisław
The aim of the study was to monitor the occurrence of selected vector-borne diseases in anaemic dogs arriving in or returning to Poland from areas endemic for these diseases.
Show more [+] Less [-]Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses
2024
Olech Monika | Kuźmak Jacek
Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat–gag (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.
Show more [+] Less [-]Effect of chokeberry pomace as a feed additive for high-producing dairy goats on oxidative stress parameters and quality of milk
2024
Szymańska-Czerwińska Monika | Matin Maima | Niemczuk Krzysztof | Strzałkowska Nina | Osiński Zbigniew | Horbańczuk Karina | Wierzbicka Agnieszka | Horbańczuk Jarosław Olav | Atanasov Atanas Georgiev | Jóźwik Artur
Chokeberry pomace, rich in polyphenolic compounds, holds potential to be a valuable feed additive for enhancing the antioxidative capacity and overall quality of milk. This study explores the impact of dietary inclusion of chokeberry pomace on oxidative stress parameters and other milk quality parameters in high-producing dairy goats.
Show more [+] Less [-]How does adulteration of wax foundation affect phenoloxidase and lysozyme activities as selected parameters of immunity in Apis mellifera?
2024
Strachecka Aneta | Chęć Magdalena | Olszewski Krzysztof | Staniszewska Patrycja | Dziechciarz Piotr | Gagoś Mariusz
The adulteration of wax foundation is, for many reasons, a growing problem of modern beekeeping not only in Europe but also around the world. Wax foundation contaminated with stearin addition leads to a brood die-off, while paraffin addition negatively affects the strength of combs. It is tenable that such adulterated wax foundation reduces bees’ immunity. The aim of the study was to determine the activities of two bee immune enzymes, lysozyme and phenoloxidase, in the haemolymph of worker bees which had emerged from combs with wax foundations contaminated with stearin or paraffin.
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