This study aimed to cultivate PK-15 cells under serum-deprived conditions and subsequently adapt a field-isolated PCV2b genotype in these adapted cells to investigate both cellular and viral changes. PK-15 cells were grown in three different serum-supplemented media: 0%, 2%, and 10%. The cells adapted to these varying serum conditions were then infected with genotyped field PCV2b and propagated for up to three blind passages. Haematoxylin and eosin (H&E) staining of PK-15 cells grown in 0% and 2% serum-supplemented media, with and without PCV2b infection, revealed cytoplasmic granularity compared to cells grown in 10% serum. MTT assay results indicated that PCV2b infection had minimal impact on cell viability, suggesting efficient viral propagation in serum-deprived conditions with minimal cytopathic effects. PCR analysis of cell lysates from all three 0%, 2%, and 10% serum conditions confirmed the presence of the PCV2b genome, demonstrating its adaptability grown to PK-15 cells under serum-deprived conditions. Molecular analysis of the major epidemiological marker gene, ORF2, from PCV2b grown in both 0% and 10% serum conditions showed identical nucleotide (702 bp) and amino acid (234 aa) compositions. This study highlights the feasibility of growing PK-15 cells under serum-deprived conditions and the adaptability of PCV2b in these cells. 

A study was conducted to assess the incidence of endoparasitism and evaluate the efficacy of anthelmintic drugs administered to captive lions housed at Arignar Anna Zoological Park, Vandalur, Tamil Nadu. A total of 10 scat samples were collected from captive lions during June-August, 2024. All the samples were examined by standard qualitative methods (direct smear, centrifugal sedimentation and floatation methods) followed by quantitative method (McMaster technique) to determine egg per gram. All the samples (n=10) showed 100% incidence of Toxascaris leonina. Efficacy of two anthelmintic drugs were evaluated by using faecal egg count reduction test (FECRT). The efficacy of ivermectin was recorded only 10.21% and found to be resistant. Fenbendazole demonstrated 100% efficacy, indicating susceptibility of the parasite to this drug.

A nine year old male Golden retriever was presented to the Small Animal Dermatology outpatient unit of Madras Veterinary College Teaching Hospital with a history of symmetrical alopecia, enlarged pendulous prepuce and haematuria. Detailed clinical examination revealed cryptorchid, gynecomastia, pendulous preputial sheath and alopecia originating in the lumbar region. The entire coat was dull, dry and epilated easily. Resting telogen  phase was also observed in trichogram. Trans abdominal palpation showed an enlarged solid mass in the right inguinal region. Radiography and abdominal ultrasonography revealed the presence of neoplastic retained right testicle. Haematology revealed leucocytosis, while serum biochemistry remained within the normal reference range. Histological examination confirmed the presence of sertoli cell tumors that were primarily responsible for the feminizing syndrome. Under general anaesthesia, caudal ceilotomy was done and retained testicles were identified. Testicular tumor with torsion was ligated with PGA 1-0 and excised, similarly the other normal testicle was also removed. Broad spectrum antibiotics and supportive therapy were also administered for 5 days. Complete remission of all clinical symptoms occurred within 3 months post orchiectomy.  

Rational drug design, is the inventive process of finding new medications based on knowledge of the biological target. Drug design involves the design of small molecules that are complementary in shape and charge to the bimolecular target to which they interact and therefore will bind to it. In the experiment based approach, drugs are discovered through trial and error. With high R&D cost and consumption, computational drug discovery helps scientists gain insight into drug receptor interactions and reduce time and cost. Scientists can predict whether the molecule will succeed or fail in the market. Currently, the process of drug designing increasingly relies on computer modeling techniques. This type of modeling is often referred to as computer-aided drug design. In computational drug discovery, different computational tools, methods, and software are used to simulate drug receptor interactions. Using computational drug discovery helps scientists gain insight into drug receptor interactions with less time and cost.

Reducing crude protein (CP) level in the feed without altering the amino acid contents by supplementing synthetic amino acids to reduce nitrogen excretion, adding ofexogenous protease enzyme infeed to improve nitrogen utilization and treating litter with the culture ofMethylomonas methanica (MM) to prevent methane emission from litter are the proven methods in commercial poultry production, especially in broiler farming to protect the environment. However, the efficacy of these nitrogen emission reduction strategies on the performance and economics of guinea fowl in intensive production has not been studied adequately. Therefore, a biological experiment was conducted with high protein (HP) diets with (Tl) or without (T2) fish meal (FM) and low protein (LP) diets added with exogenous protease enzyme with (T3) or without (T4) FM. The levels of critical amino acids in all the feed were balanced with the supplementation of synthetic amino acids. These treatments contained six replicates of each with 15-day-old keets. At the start of ninth week, the litter of three replicates from each treatment was sprayed with MM culture additionally, forming four more treatment groups, from T5 to T8 respectively. The results of the study indicated that replacing FM with plant protein sources did not affect body weight. The overall trend revealed that the reduction of CP by 2% had a negative (P<0.05) impact on body weight; while, the addition of protease in feed had no notable effect. The feed consumption was not affected by the withdrawal of FM or reducing CP level by 2% ordietary protease supplementation or MM litter treatment. The feed efficiency was negatively impacted (P<0.05) by withdrawal ofFMin high and low protein diets; -while, feed efficiency -was not improved by protease supplementation in LP diets. The level of CP or -withdrawal FM or protease enzyme supplementation in the ration did not affect livability. The MM litter treatment did not affect gro-wth rate, feed consumption, feed efficiency and livability of guinea fowls. The results indicated that the benefit-cost ratio could be improved in guinea fowls by using low CP feed.

Ricinoleic acid, a toxic compound found in castor seeds (Ricinus communis L.), can be lethal when inhaled, ingested, or injected at doses of 5-10 pg/kgto human or animals. This study presents a validated, rapid, and precise high-performance thin-layer chromatographic (HPTLC) methodfor its quantification. Separation was achieved on silica gel 60 F254plates using a mobile phase oftoluene, ethyl acetate and acetate acid (6:4:0.2 v/v/v). Densitometric scanning at 450 nm detected ricinoleic acid with an Rfvalue of 0.66 (±0.04). Linearity was observed in the range of 20-100 ng/spot with R = 0.998 ± 2.62%.  

Gymnema sylvestre, Annona muricata and Ocimum sanctum are well known plants available in India and they are commonly used for the treatment of various diseases including diabetes mellitus. A study was conducted to assess the hypoglycemic effect of a polyherbal extract containing these plants (at two different doses of300 and 600 mg/kg bwt) compared with metformin as a standard hypoglycemic drug at a dose of 100 mg/kg bwt in streptozotocin-induced diabetes in rats. As a part of the study, the effect of the polyherbal extract on the renal and hepatic functions was tested in rats. The serum parameters both ALT and AST values in rats were significantly (p < 0.05) higher in diabetic rats treated with polyherbal extract at 300 mg/kg bwt when compared to Groups I, II and III. BUN values were significantly higher in diabetic and metformin treated groups. Creatinine did not differ significantly among different groups. In conclusion, in streptozotocin induced diabetic rats, the polyherbal extract at 300 and 600 mg/kg bwt had no hepatoprotective action. But the same doses had some renal protective function.

Organophosphates (OP) and carbamates are widely utilized insecticides, but they also pose significant risks as sources ofintoxicationfor both humans and animals. A male non-descript dog, seven years old was presented with a chronic respiratory distress, dysphonia, exercise intolerance, and neurologic signs to Veterinary Clinical Complex, VCRI, Orathanadu on April 2023. Physical examination revealed elevated rectal temperature and congested mucous membrane with elevated heart beat and respiratory rate. Thoracic auscultation revealed muffled heart sound. The dog's medical history indicated a potential case of organophosphate poisoning. Treatment involved administering atropine sulfate, normal saline, ceftriaxone, and furosemide. However, throughout the treatment process, the dog continued to experience upper respiratory stridor and dyspnoea. Clinical examination and radiographic observations confirmed the diagnosis of laryngeal paralysis. This case highlights the occurrence of laryngeal paralysis polyneuropathy complex in a canine affected by organophosphate poisoning.

A one and half year, female, non descriptive dog was presented to Veterinary College Hospital, Hassan with the history of incoordination, drowsiness and vomiting after accidental oral administration ofAmitraz liquid (RIDD®) by the owner. On physical examination animal was dull and depressed. Sedation (drowsiness) and hypothermia was noticed with the rectal temperature of 98.8°E Hemato-biochemical findings were within the normal range. Based on history and clinical signs, the case was diagnosed as amitrazpoisoning and animal was treated with Yohimbine Hydrochloride at the rate of 0.2 mg per Kg body weight intramuscularly and activated charcoal per orally, along with the supportive therapy. Animal was completely recovered after 5 days of treatment.

A eight year old unspayed female Labrador dog weighing 20 kg was presented with a history of dehydration, vomiting, pyrexia, anorexia. General examination revealed that animal was depressed,with abdominal distention, sanguine purulent vaginal discharge, anuria and inability to walk. On clinical examination, the rectal temperature was recorded as 101.3oF, with swollen vulva and seropurulent vaginal discharge. On haematological examination revealed neutrophilia with left shift. The bitch was treated with one shot of cloprostenol sodium with dose rate 2 mcg/kg intramuscularand inj. cefotaxime sodium 250 mg for 5 days. But, it failed to respond the treatment. Based on the history, radiography and clinical observations the condition was diagnosed as pyometra. Hence ovariohysterectomy was performed under general anaesthesia and the animal recovered eventfully.