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Antimicrobial susceptibility and genetic characteristics of Streptococcus uberis isolated from bovine mastitis milk
2007
Lee, G. (Konkuk University, Seoul, Republic of Korea) | Kang, H.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Chung, C.I. (Konkuk University, Seoul, Republic of Korea) | Moon, J.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected]
Streptococcus spp. comprising Streptococcus (S.) uberis and S. dysgalactiae strains is major cause of bovine mastitis from particularly well-managed or low somatic cell count herds that have successfully controlled contagious pathogens. In this study, antimicrobial susceptibility and genetic characteristics of S. uberis isolated from clinical or subclinical mastitis milk at 2003 were investigated. Eighty seven isolates of Streptococcus spp. were identified by the conventional biochemical methods. The antimicrobial susceptibility by disk diffusion method was determined for 46 S. uberis, 11 S. bovis, 10 S. oralis, 6 S. uberis and 14 other Streptococcus spp., Overall, the tested strains were susceptible to tetracycline (11.5%), amikacin (14.9%), streptomycin (16.1%), neomycin (26.4%), kanamycin (35.6%), gentamicin (65.2%), oxacillin (70.1%), ampicillin (75.9%), chloramphenicol (78.2%), and cephalothin (97.7%).
Show more [+] Less [-]Establishment of a statistically reliable sampling method and size for serological surveillance of classical swine fever (CSF) in Korea
2007
Yoon, H.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Nam, H.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Park, C.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Park, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Hyeon, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Wee, S.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected]
To establish a statistically reliable sampling strategy for serological surveillance of classical swine fever (CSF) in Korea, antibody test data from CSF surveillance conducted during year 2005 were analyzed. The most appropriate sampling method was determined to be stratified multi-stage random sampling strategy, in which the primary sampling unit is a pig farm and the secondary are the pigs by the strata of breeders and finishers in the selected farm. The optimum sample size was 5 to 19 including 1 to 2 breeders according to the number of pigs in the farm.
Show more [+] Less [-]Multicentric T cell lymphoma in a Maltese dog
2007
Jung, J.Y. (Cheju National University, Jeju, Republic of Korea) | Kang, S.C. (Cheju National University, Jeju, Republic of Korea) | Roh, I.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Sohn, H.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yun, Y.M. (Cheju National University, Jeju, Republic of Korea) | Kim, J.H. (Cheju National University, Jeju, Republic of Korea) | Lee, K.K. (Cheju National University, Jeju, Republic of Korea) | An, M.C. (Halla Pet Hospital, Jeju, Republic of Korea) | Bae, J.H. (Cheju National University, Jeju, Republic of Korea) | Kim, J.H. (Cheju National University, Jeju, Republic of Korea), E-mail: [email protected]
A case of multicentric high grade T cell lymphoma is reported in a 5-year-old male Maltese dog with generalized lymphadenopathy. The dog showed depression, anorexia, blindness, jaundice, arrhythmia, and hematuria for 8 months. Complete blood count and chemistry profile revealed anemia and increased alanine transferase, alkaline phosphatase, total bilirubin, and total cholesterol. Grossly, most of lymph nodes, spleen, and liver were enlarged and neoplastic masses were occupied in these tissues. Histologically, massive accumulation of small noncleaved neoplastic lymphocytes with high mitotic figures was observed in all lymph nodes and spleen.
Show more [+] Less [-]Successful pregnancy following surgical intrauterine insemination using frozen semen in Afghan hound dog
2007
Kim, H.J. (Seoul National University, Seoul, Republic of Korea) | Oh, H.J. (Seoul National University, Seoul, Republic of Korea) | Jang, G. (Seoul National University, Seoul, Republic of Korea) | Yoon, J.H. (Seoul National University, Seoul, Republic of Korea) | Lee, H.S. (Woosong Information College, Daejeon, Republic of Korea) | Kim, S.C. (Shingu College, Seongnam, Republic of Korea) | Hwang, C.Y. (Seoul National University, Seoul, Republic of Korea) | Kim, D.Y. (Seoul National University, Seoul, Republic of Korea) | Kim, M.K. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was 59.4×10∨6 and the total volume was 1.2 ml. The frozen spermatozoa were thawed in 70℃ water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method.
Show more [+] Less [-]Evaluation and modification of alkaline lysis plasmid preparation method from Lactobacillus spp.
2007
Lee, D.Y. (Seoul National University, Seoul, Republic of Korea) | Seo, Y.S. (Seoul National University, Seoul, Republic of Korea) | Kang, S.G. (Seoul National University, Seoul, Republic of Korea) | Yoo, H.S. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed is isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method.
Show more [+] Less [-]Cycad revoluta toxicosis in a dog
2007
Yoo, J.H. (Konkuk University, Seoul, Republic of Korea) | Kim, H.T. (Leesangyoon Animal Hospital, Seoul, Republic of Korea) | Park, C. (Konkuk University, Seoul, Republic of Korea) | Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Jung, D.I. (Konkuk University, Seoul, Republic of Korea) | Woo, E.J. (Kyung Hee University, Yongin, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
A 3-year-old female Cocker Spaniel dog acutely developed hepatopathy following ingestion of root of Cycad (Cycas revoluta). Seven hours after the ingestion, she showed acute continuous nausea, vomiting, and marked depression and was presented to the local veterinary clinic. At physical evaluation, the dog showed moderate weakness, pale mucous membrane, jaundice, and dehydration. Serum biochemical analysis revealed elevated alanine aminotransferase and aspartate aminotransferase, and mild azotemia. The dog was treated with intravenous fluid, antibiotics, and hepatic protectants for 10 days and recovered without any sequela.
Show more [+] Less [-]Iatrogenic hydroureter and hydronephrosis following hysterotomy with ovariohysterectomy in a pregnant dog
2007
Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Park, C. (Kyung Hee University, Yongin, Republic of Korea) | Jung, D.I. (Konkuk University, Seoul, Republic of Korea) | Yoo, J.H. (Konkuk University, Seoul, Republic of Korea) | Kim, J.W. (Konkuk University, Seoul, Republic of Korea) | Gu, S.H. (Konkuk University, Seoul, Republic of Korea) | Jeon, H.W. (Konkuk University, Seoul, Republic of Korea) | Kim, J.H. (Konkuk University, Seoul, Republic of Korea) | Woo, E.J. (Kyung Hee University, Yongin, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
A 14-month-old, neutered female Miniature Pinscher was referred due to severe renal failure. The dog was treated with hysterotomy and followed by ovariohysterectomy (OHE) 6 days before the presentation. Excretory urographic and ultrasonographic studies revealed bilateral hydroureter and hydronephrosis. Postmortem necropsy revealed that ligatures were placed around the caudal portion of bilateral ureters in front of entrance into the bladder. This case shows that identifying and protecting the ureters are important during hysterotomy with OHE as well as OHE.
Show more [+] Less [-]CT characteristics of normal canine pulmonary arteries and evaluation of optimal contrast delivery methods in CT pulmonary angiography
2007
Jung, J.H. (Seoul National University, Seoul, Republic of Korea) | Chang, J.H. (Seoul National University, Seoul, Republic of Korea) | Yoon, J.H. (Seoul National University, Seoul, Republic of Korea) | Choi, M.C. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
This study was performed to identify the normal anatomic orientation of pulmonary arteries and to obtain the normal baseline parameters and the optimal contrast material delivery methods of computed tomographic pulmonary angiography (CTPA) on normal beagle dogs. Based on the contrast injection flow rate, the contrast volume, and the administration methods, the experimental groups were divided into 4 groups such as group 1 : 2 ml/s, 3 ml/㎏, and monophasic administration; group 2 : 5 ml/s, 3 ml/㎏, and monophasic administration; group 3 : 5 ml/s, 4 ml/㎏, and monophasic administration; group 4 : 5 ml/s and 2 ml/㎏ in first phase, 0.3 ml/s and 2 ml/㎏ in second phase, as biphasic administration.
Show more [+] Less [-]Evaluation on efficacy of β-hemolytic Streptococcus iniae vaccine on olive flounder, Paralichthys olivaceus
2007
Moon, J.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Jang, H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Joh, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, M.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Son, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of β-hemolytic Streptococcus (S.) iniae vaccine on olive flounder.
Show more [+] Less [-]Malignant aortic body tumor in a Korean Jindo dog
2007
Hwang, D.N. (Konkuk University, Seoul, Republic of Korea) | Kang, B.T. (Konkuk University, Seoul, Republic of Korea) | Hur, T.Y. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Chang, B.J. (Konkuk University, Seoul, Republic of Korea) | Park, H.M. (Konkuk University, Seoul, Republic of Korea) | Yhee, J.Y. (Konkuk University, Seoul, Republic of Korea) | Yu, C.H. (Konkuk University, Seoul, Republic of Korea) | Sur, J.H. (Konkuk University, Seoul, Republic of Korea), E-mail: [email protected]
A-7-year-old, intact female Korean Jindo dog was referred due to ataxia and pain on palpation of the thorax. Radiograph, echocardiography and computed tomogram revealed a mass on the heart base region and osteolytic change of fifth thoracic (T5) vertebra. At necropsy, a firm, encapsulated and round mass was seen arising from the heart base region surrounding the ascending aorta and pulmonary artery. Histopathologically, nests of cuboidal and polyhedral cells having abundantly granular and eosinophilic cytoplasm with round to oval nucleus were separated by fibrous septa.
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